Oliver C. Kiersnowski1, Patrick Fuchs1, Stephen J. Wastling2,3, John S. Thornton2,3, and Karin Shmueli1
1Department of Medical Physics and Biomedical Engineering, University College London, London, United Kingdom, 2Neuroradiological Academic Unit, UCL Queen Square Institute of Neurology, University College London, London, United Kingdom, 3Lysholm Department of Neuroradiology, National Hospital for Neurology and Neurosurgery, London, United Kingdom
Synopsis
Keywords: Susceptibility, Quantitative Susceptibility mapping
Echo planar imaging (EPI) suffers from geometric distortions, which can
be corrected using reference acquisitions for single-echo EPI, or using field
maps calculated from the multiple echoes in multi-echo EPI. The effect of
distortion on EPI quantitative susceptibility mapping (QSM) has not been
investigated. We compared static and dynamic geometric distortion correction
for magnitude images and QSM using multi-echo 2D-EPI acquisitions with and
without changes in head position. Multi-echo EPI corrected distortion in both
magnitude and QSM images without the need for reference scans. Correcting the
local field map in the QSM pipeline was optimal to reduce temporal variance.
Introduction
Echo planar imaging (EPI) has been used for structural1 and functional2–4 quantitative susceptibility mapping
(QSM)5, but suffers from geometric distortion.
Single-echo EPI distortion correction can be applied using a static field map
obtained from a reference scan6,
or dynamically using field maps estimated for each timepoint7,8. Multi-echo (ME) EPI has the
advantage that dynamic field maps can be calculated by fitting the phase over
multiple echo times9, obviating any reference scans. It is
unknown where in the QSM pipeline to apply distortion correction optimally. Therefore,
we applied static and dynamic distortion correction at three points in the QSM
pipeline to investigate the effect of distortion on the accuracy of
susceptibility ($$$\chi$$$) values and to determine the optimal point to apply
correction.Methods
Acquisition
A healthy volunteer was imaged on a 3T Siemens Prisma
MR system using a 64-channel head coil. A reference 3D-GRE scan was acquired at
1mm isotropic resolution with echo times: TE
1/ΔTE/TE
4=4.92/4.92/19.68 ms; TR=30 ms; GRAPPA=3; 6/8 phase partial
Fourier; FOV=256x256x192 mm; T
tot=7min 25s.
A single-shot 2D-EPI
10 scan was used twice to acquire 70
timepoints at 1.5 mm isotropic resolution with TE=12.8, 33.71, 54.62 ms;
TR=3346 ms; GRAPPA=4; multiband factor 3; T
tot=5min11s. At three times during
the second run, the volunteer changed their head position, causing dynamic
geometric distortions and allowing static and dynamic correction methods to be
compared.
A standard structural reference image was acquired
using a T1-weighted MPRAGE sequence at 1mm isotropic resolution.
Distortion correction
Using FSL FUGUE
11, three methods were applied to correct
the BOLD-optimised combined
12 magnitude images, and QSMs at three
points in the QSM pipeline: (i) the total field map (TFM), (ii) local field map
(LFM) and (iii) the final susceptibility map (FSM), using the following field
maps:
- sGRE: a static, reference field map calculated from the
3D-GRE acquisition was applied to each EPI timepoint.
- sEPI: a static field map calculated from the first EPI
timepoint was applied to each subsequent timepoint.
- dEPI: dynamic field maps were calculated and applied at each
EPI timepoint.
Field map calculation
The static field map for
sGRE was calculated
using a non-linear fit
13 of the GRE complex data over all TEs with
residual phase wraps unwrapped using SEGUE
14. This was registered to each EPI volume
using an affine transformation obtained by rigidly registering the first echo
magnitude images of the GRE and EPI with NifyReg
15, followed by ‘forward warping’ the GRE
map into the EPI space by applying the inverse of the field map’s distortion
correction to itself
8.
Field maps for
sEPI and
dEPI were calculated
at each EPI volume using a non-linear fit
13 of the field-corrected
16 EPI complex data over all TEs and SEGUE
14 phase unwrapping. The first volume EPI
field map was used for
sEPI.
Brain masks for all field maps were obtained using FSL
BET
17 on the first-echo magnitude images, from
their respective GRE/EPI acquisition. To improve distortion correction at the
edges of the brain, regions outside the brain were set to NaNs and field maps
were extrapolated well beyond the brain edges using MATLAB function smoothn.m
18 with smoothing parameter=2, as in
Dymerska et al.
8.
QSM pipeline
GRE and EPI $$$\chi$$$ maps were reconstructed via Laplacian
unwrapping
19 of the fitted field maps used in the
field map calculations, followed by PDF
20 background field removal (plus 2D/3D
V-SHARP for EPI
21,22) and non-linear total variation $$$\chi$$$ calculation
23,24 (α=2×10
-5 and α=1×10
-4 for GRE and EPI, respectively). Average
susceptibility maps were calculated by averaging maps that were initially co-registered
to the first volume across all (non-motion corrupted) timepoints.
Statistical Analysis
The temporal standard deviation ($$$\sigma$$$/$$$\sigma_\chi$$$) between uncorrected and corrected magnitude
images / susceptibility maps were compared visually and using Kruskal-Wallis
tests to identify differences in the medians of brain $$$\sigma$$$ distributions by statistically comparing the
mean ranks of the distributions. Mean $$$\chi$$$ values within five ROIs (segmented using
MRICloud
25,26) were compared for all correction
methods.
Results and Discussion
Head position changes led to
different distortions (Figure 1, top row), which were corrected well by static
field maps obtained in the same position (Figure 1, left) but were poorly
corrected at a different position (Figure 1, right). Dynamic EPI field maps (dEPI)
accurately corrected distortion in all positions (Figure 1, bottom row) and reduced $$$\sigma$$$ (Figure 2a). $$$\sigma$$$ was reduced the most by sGRE when the
subject’s head did not change position but by dEPI when the head did
change position (Figure 2b). Head movement led to large regional $$$\chi$$$ shifts that were not removed by distortion
correction (Figure 3). However, all correction methods reduced $$$\sigma_\chi$$$ (Figure 4) compared to the uncorrected $$$\chi$$$ map. dEPI reduced $$$\sigma_\chi$$$ inside the brain more than sGRE and sEPI
(Figure 4, yellow arrows). Distortion correction of the LFM reduced $$$\sigma_\chi$$$ the most for all correction methods, while
correcting the FSM reduced $$$\sigma_\chi$$$ the least (Figure 5a). Corrected averaged
susceptibility maps display small differences compared to uncorrected (Figure
5b).Conclusions
Dynamic distortion
correction from ME-EPI reduced distortion in the magnitude and $$$\chi$$$ maps without need for additional reference
scans. Distortion correction applied to the local field map reduced temporal
variability in $$$\chi$$$ the most, which is relevant for functional QSM
applications.Acknowledgements
Oliver Kiersnowski’s work
was supported by the EPSRC-funded UCL Centre for Doctoral Training in
Intelligent, Integrated Imaging in Healthcare (i4health) (EP/S021930/1). John
Thornton received support from the National Institute for Health Research
University College London Hospital Biomedical Research Centre. Karin Shmueli
and Patrick Fuchs were supported by European Research Council Consolidator
Grant DiSCo MRI SFN 770939.References
- Sun H, Wilman AH. Quantitative
susceptibility mapping using single-shot echo-planar imaging. Magn Reson Med.
2015;73(5):1932-1938. doi:10.1002/mrm.25316
- Balla DZ, Sanchez-Panchuelo RM,
Wharton SJ, et al. Functional quantitative susceptibility mapping (fQSM). Neuroimage.
2014;100:112-124. doi:10.1016/j.neuroimage.2014.06.011
- Sun H, Seres P, Wilman AH. Structural
and functional quantitative susceptibility mapping from standard fMRI studies. NMR
Biomed. 2017;30(4). doi:10.1002/nbm.3619
- Özbay PS, Warnock G, Rossi C, et al.
Probing neuronal activation by functional quantitative susceptibility mapping
under a visual paradigm: A group level comparison with BOLD fMRI and PET. Neuroimage.
2016;137:52-60. doi:10.1016/j.neuroimage.2016.05.013
- Shmueli K. Quantitative
Susceptibility Mapping. In: Quantitative Magnetic Resonance Imaging. 1st
ed. Elsevier; 2020.
- Jezzard P, Balaban RS. Correction for
geometric distortion in echo planar images from B0 field variations. Magn
Reson Med. 1995;34(1):65-73. doi:10.1002/mrm.1910340111
- Dymerska B, Poser BA, Bogner W, et
al. Correcting dynamic distortions in 7T echo planar imaging using a jittered
echo time sequence. Magn Reson Med. 2016;76(5):1388-1399.
doi:10.1002/mrm.26018
- Dymerska B, Poser BA, Barth M,
Trattnig S, Robinson SD. A method for the dynamic correction of B0-related
distortions in single-echo EPI at 7 T. Neuroimage. 2018;168:321-331.
doi:10.1016/j.neuroimage.2016.07.009
- Hutton C, Bork A, Josephs O,
Deichmann R, Ashburner J, Turner R. Image distortion correction in fMRI: A
quantitative evaluation. Neuroimage. 2002;16(1):217-240.
doi:10.1006/nimg.2001.1054
- Center for Magnetic Resonance Research
Department of Radiology. Multi-Band Accelerated EPI Pulse Sequences.
https://www.cmrr.umn.edu/multiband/
- Jenkinson M, Beckmann CF, Behrens TEJ,
Woolrich MW, Smith SM. FSL. Neuroimage. 2012;62(2):782-790.
doi:10.1016/j.neuroimage.2011.09.015
- Poser BA, Norris DG. Investigating the
benefits of multi-echo EPI for fMRI at 7 T. Neuroimage.
2009;45(4):1162-1172. doi:10.1016/j.neuroimage.2009.01.007
- Liu T, Wisnieff C, Lou M, Chen W,
Spincemaille P, Wang Y. Nonlinear formulation of the magnetic field to source
relationship for robust quantitative susceptibility mapping. Magn Reson Med.
2013;69(2):467-476. doi:10.1002/mrm.24272
- Karsa A, Shmueli K. SEGUE: A Speedy
rEgion-Growing Algorithm for Unwrapping Estimated Phase. IEEE Trans Med
Imaging. 2019;38(6):1347-1357. doi:10.1109/TMI.2018.2884093
- Modat M, Cash DM, Daga P, Winston GP,
Duncan JS, Ourselin S. Global image registration using a symmetric block-matching
approach. Journal of Medical Imaging. 2014;1(2):024003.
doi:10.1117/1.jmi.1.2.024003
- MEDI Toolbox.
http://pre.weill.cornell.edu/mri/pages/qsm.html
- Smith SM. Fast robust automated brain
extraction. Hum Brain Mapp. 2002;17(3):143-155. doi:10.1002/hbm.10062
- Garcia D. Robust smoothing of gridded
data in one and higher dimensions with missing values. Comput Stat Data Anal.
2010;54(4):1167-1178. doi:10.1016/j.csda.2009.09.020
- Schofield MA, Zhu Y. Fast phase
unwrapping algorithm for interferometric applications. Opt Lett.
Published online 2003. doi:10.1364/ol.28.001194
- Liu T, Khalidov I, de Rochefort L, et
al. A novel background field removal method for MRI using projection onto
dipole fields (PDF). NMR Biomed. 2011;24(9):1129-1136.
doi:10.1002/nbm.1670
- Wei H, Zhang Y, Gibbs E, Chen NK, Wang
N, Liu C. Joint 2D and 3D phase processing for quantitative susceptibility
mapping: application to 2D echo-planar imaging. NMR Biomed. 2017;30(4).
doi:10.1002/nbm.3501
- Li W, Wu B, Liu C. Quantitative
susceptibility mapping of human brain reflects spatial variation in tissue
composition. Neuroimage. 2011;55:1645-1656.
doi:10.1016/j.neuroimage.2010.11.088
- Milovic C, Bilgic B, Zhao B,
Acosta-Cabronero J, Tejos C. Fast nonlinear susceptibility inversion with
variational regularization. Magn Reson Med. 2018;80(2):814-821.
doi:10.1002/mrm.27073
- FANSI Toolbox.
https://gitlab.com/cmilovic/FANSI-toolbox
- Li X, Chen L, Kutten K, et al.
Multi-atlas tool for automated segmentation of brain gray matter nuclei and
quantification of their magnetic susceptibility. Neuroimage.
2019;191:337-349. doi:10.1016/j.neuroimage.2019.02.016
- Mori S, Wu D, Ceritoglu C, et al.
MRICloud: Delivering high-throughput MRI neuroinformatics as cloud-based
software as a service. Comput Sci Eng. 2016;18(5):21-35.
doi:10.1109/MCSE.2016.93