The talk will propose an overview of the mainly available pipelines to assess longitudinal brain structural changes and to focus on the best practice to perform these quantifications. In the first part, we will introduce the basic concepts on the research questions and technical aspects to guide in the selection of the most suitable longitudinal pipeline among the available ones. In the second part we will see practically step-by-step how to perform a global and regional assessment of brain volumetric changes, using an open-access dataset. The participants will finally be able to independently perform such analyses and critically interpret it.