Imaging metabolic pathways by using non-invasive techniques has always been a challenge overcoming which may lead to opportunities for better diagnosis, planning a personalized treatment regimen, and evaluation of treatment response. Clinical translation of the multinuclear approaches combined with the administration of stable isotopes, such as 13C/2D labeled glucose, is yet to be widely utilized in clinical and preclinical studies. In this talk, I will discuss the development of 13C and 2D MRS techniques through the years and their potential future applications.