Shota Ishida1, Hirohiko Kimura2, Naoyuki Takei3, Yasuhiro Fujiwara4, Tsuyoshi Matsuda5, and Nobuyuki Kosaka2
1Department of Radiological Technology, Faculty of medical sciences, Kyoto College of Medical Science, Nantan, Japan, 2Department of Radiology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan, 3GE Healthcare, Tokyo, Japan, 4Department of Medical Image Sciences, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan, 5Division of Ultra-high Field MRI, Institute for Biomedical Science, Iwate Medical University, Shiwa-gun, Japan
Synopsis
This study aimed
to assess whether the efficiency of DANTE, a vascular suppression in ASL, is
affected by vascular territories. CBF, transit times (TT), and their rates of
change were determined in nine vascular territories from the proximal to distal
regions. Although CBF reduction rates were not different among the territories,
TT extension rates were lower in the distal region than in other regions. No correlation
was observed between TT extension and CBF reduction rates. DANTE efficiency is
not affected by transit time or vascular territories. Homogenous vascular
suppression effects could be obtained by DANTE-ASL.
Introduction
Delays alternating with nutation for
tailored excitation (DANTE) pulse1 is a vascular suppression (VS)
technique of arterial spin labeling (ASL)2. The right-left differences in
the DANTE-ASL
signals were smaller than those in the motion-sensitized driven-equilibrium2.
Furthermore, the DANTE efficiency is associated with velocity3. In our recent study, we demonstrated
that DANTE could selectively eliminate microvascular signals and separate the
spin compartments in the ASL4. Further studies have shown that a
combination of a simplified two-compartment model and DANTE-ASL could measure
arterial cerebral blood volume (CBVa)4.
CBVa measurement using
DANTE-ASL can be utilized to monitor cerebral hemodynamics in patients with
chronic cerebrovascular diseases (CVDs), such as Moyamoya disease. In the early
stages of CVDs, elevated CBVa is a typical feature necessary for
maintaining cerebral blood flow (CBF) by compensating for decreased cerebral
perfusion pressure. A remarkable extension of the arterial transit time
(ATT) was observed in patients with CVDs5, and this factor is known
to depend on the vascular
territories, even in healthy volunteers5,6. Thus, the DANTE efficiency could be
affected by the ATT
because of its velocity sensitivity. This may reduce the clinical reliability
of CBVa measurements using DANTE. Robustness to changes in ATT is a
requirement for the clinical
application of DANTE. Therefore, in the present study, we assessed whether
DANTE efficiency was affected by vascular territories.Methods
We performed additional quantitative
analyses on previously acquired data, in which sequential (n = 11) and
Hadamard-encoded (n = 10) acquisitions were conducted on a 3.0-T MRI scanner (Discovery MR750, GE Healthcare, USA).
DANTE was set as the previous
condition (flip angle of 12.5° and gradient area of 10
µs T/m)3. For the sequential
multidelay, 2.4 s labeling
duration (LD) and three post-labeling delays (PLDs) were used (0.4, 1.2, and 2.0 s for non-DANTE and
0.8, 1.2, and 1.6 s for
DANTE scans, respectively). For the Hadamard multidelay, 3.6 s LD was segmented
into three LD blocks (0.7, 0.9, and 2.0 s). PLDs were 0.4 and 0.8 s for the
non-DANTE and DANTE, respectively. An extra-encoding step in the Hadamard
matrix enabled the computation of an additional ASL image with the entire LD
(3.6 s) and PLDs (0.4 and 0.8 s) of the Hadamard setting. 3D-T1
weighted images were also obtained.
Coregistration was
performed on all the ASL images using individual 3D-T1
images. The signal-weighted delay method was used to estimate the initial transit time (TT)7. Subsequently, TT and CBF were
calculated on a voxel-by-voxel basis using a least-squares solution with a
single-compartment model. Consequently, we obtained TT without DANTE (TTnoVS),
TT with DANTE (TTDANTE), CBF without DANTE (CBFnoVS), and
CBF with DANTE (CBFDANTE). All parameter maps were spatially
normalized to MNI space. White matter regions were excluded from the
measurement target because of the low reliability of the
ASL signals4. Based on the vascular territory atlas8, TTnoVS, TTDANTE, CBFnoVS,
and CBFDANTE were determined by nine volumes of interest (VOIs), in
which the anterior cerebral artery (ACA), middle cerebral artery
(MCA), and posterior cerebral artery (PCA) were divided into three regions each (proximal, middle, and distal). The TT and CBF values were assessed for each territory, and the rates of TT
extension and CBF reduction were evaluated.Results and Discussion
Table 1 shows the CBF,
TT, and their rates of change. Similar trends were observed for the CBF reduction and TT extension rates between
the multidelay techniques. In both multidelay
methods, the TT extension rates were lower in the distal region than in others.
These findings indicate that the DANTE efficiency is independent of the
multidelay method. CBF reduction and TT extension rates were smaller with
sequential as compared to those with Hadamard (Table 1 and Figure 1).
Figure 2 shows the CBF and TT maps
calculated from each multidelay dataset using a single-compartment model. Shorter
TTnoVS and TTDANTE were observed with Hadamard compared
to the sequential. This could be attributed to the difference in LDs between
the methods. While sequential multidelay uses 2.4 s LDs for all the boluses,
Hadamard LDs were 0.7, 0.9, and 2.0 s. Shorter LDs of the Hadamard led to
reduced signal-to-noise ratio (SNR), which could cause TT underestimation5. Inaccurate TTs make the CBF
measurement inaccurate. Differences in the bolus design could cause different
baseline TT and CBF values, which could result in differences in the rates of
change between the techniques.
Figure 3
shows the relationship
between TT extension and CBF reduction rates. No correlation was observed.
Figure 4 also shows that TTnoVS and TTDANTE were not
correlated with the CBF reduction rate (i.e., degree of signal
reduction by DANTE), which indicates that the DANTE efficiency is not affected
by TT (i.e., vascular territories). In contrast, TT extension rates were
negatively correlated with TTnoVS. This could be due to the smaller CBVa in the peripheral regions because the vessel size and fraction in a voxel decreased approaching the periphery.
Thus, we considered that this was due to physiological vascularity rather than
DANTE efficiency.Conclusion
DANTE efficiency
is not affected by transit time or vascular territories. Homogenous VS effects
were observed by DANTE-ASL in all regions of the brain.Acknowledgements
No acknowledgement found.References
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