Su Dongning1,2, Zhang Zhe2,3, Zhang Zhijin1,2, Zhu Wanlin2,3, Sui Binbin2,3, Zhang Yingkui2,3, Bi Jingfeng2,3, Kong Qingle 3,4, Gan Yawen1,2, Yan Rui1,2, Wang Xuemei1,2, Wang Zhan1,2, Wang Yongjun 1,2,3, Wu Tao1,2, Jing Jing1,2,3, and Feng Tao1,2
1Department of Neurology, Beijing Tiantan Hospital,Captital Medical University, Beijing, China, 2China National Clinical Research Center for Neurological Diseases, Beijing, China, 3Tiantan Neuroimaging Center of Excellence, Beijing Tiantan Hospital, Capital Medical University, Beijing, China, 4MR Collaboration, Siemens Healthineers Ltd., Beijing, China
Synopsis
To explore specific neuroimaging
characteristics of iron deposition in WD, we conducted a small sample, prospective
case-control study to compare the 7T MRI manifestation of iron deposition in the
basal ganglia among PD, WD patients and healthy controls. The hyperintense
globus pallidus rim sign could be a specific neuroimaging characteristic of WD
in 7T T2*-weighted and SWI images, which may be related to the unique iron
deposition pattern in the striatum in WD patients. Internal capsule appears to
be hyperintensity with a clear border only observed in WD patients. These
findings have the potential to provide diagnostic biomarkers for WD.
Introduction
Wilson disease
(WD), also referred to as hepatolenticular degeneration, is a genetic disorder
related to the mutation of ATP7B copper transporter. WD is characterized by ataxia
and other movement disorders caused by disturbances of the extrapyramidal system.
Previous pathological studies have shown that iron deposition
instead of copper deposition is related to T2/T2*w in the basal ganglia
hypointensity1.
Similar to WD, PD is also a movement disorder with iron deposition
in the basal ganglia. Compared with 3T MRI, 7T MRI could provide higher
sensitivity for detecting iron concentration changes. It is unknown whether there
exist distinct image charateristics on 7T MRI between the two diseases. To address
this question, we conducted a small sample, prospective case-control study to compare
the 7T MRI manifestation of iron deposition in the basal ganglia among PD, WD patients
and healthy controls, aimed to explore specific neuroimaging characteristics
of iron deposition in WD. Methods
Four WD patients, four
PD patients and four age-matched healthy controls were recruited. The study was
approved by the local Ethical Standards Committee, and written informed consent
was obtained from all participants. All the subjects underwent 7T MR (MAGNETOM
Terra, Siemens Healthcare, Erlangen, Germany) scans with three-dimensional (3D)
T2*-weighted Imaging (transverse scan,
4-echo 3D GRE, acquisition voxel = 0.4×0.4×0.5mm3, 88 slices, TE = 8/15/22/29 ms, TR =
42ms, flip angle = 16°, acceleration = 3, scan time = 8:24), two-dimensional
(2D) T2*-weighted Imaging (transverse scan, 2-echo 2D GRE, acquisition voxel =
0.2×0.2×1.2mm3, 29 axial slices, TE = 15/30 ms, TR = 1400ms,
flip angle = 50°,
acceleration = 2, scan time = 7:20) and 3D SWI (transverse scan, acquisition
voxel = 0.3×0.3×1.2mm3,
104 slices, TE = 12 ms, TR = 19 ms, flip angle = 14°,
acceleration = 3, scan time = 6:47). The observation of MRI was conducted by
two experienced neurologists.Results
Compared with
healthy controls, relatively low intensity in the putamen, globus pallidus and
caudate could be observed in both WD and PD patients in SWI and T2*w sequence,
which was related to iron concentration in neurodegenerative disorders.
However, the patterns of iron deposition were different between WD and PD. The
hypointensity in the thalamus was only observed in WD patients, and the
internal capsule appears to be hyperintensity with a clear border (Figure 1). Moreover,
bilateral linear signal intensity changes consisting of a lateral hyperintense
rim at the lateral border of globus pallidus could only be observed in WD
patients. We term the intensity change as the hyperintense globus pallidus rim
sign, which was absent in PD and healthy controls in this study (Figure 1). A sketch
map of hyperintense globus pallidus rim sign is shown in Figure 2.Discussion
To our best
knowledge, this might be the first 7T MRI study focusing on the visible
neuroimaging biomarker for the diagnosis of WD. In this preliminary
case-control study, we found that the hyperintense globus pallidus rim sign
could be a specific neuroimaging characteristic of WD. According to a
post-mortem 7T MRI-histopathological study, T2/T2*w hypointensity observed in
vivo in the basal ganglia of WD patients is related to the increased iron
concentration rather than copper deposition1.
Therefore, the hyperintense globus pallidus rim sign may be related to the unique
iron deposition pattern in the striatum in WD patients, which is worth
investigating in the histological study. Moreover, the relatively high intensity
with a clear border of the internal capsule in WD can be associated with the
increased iron deposition in globus pallidus, caudate and thalamus2.
These findings have the potential to provide diagnostic biomarkers for WD.
Future larger cohort study is needed to confirm the results.Conclusion
Using 7T MRI, our pilot
case-control study suggested the hyperintense globus pallidus rim sign and
relatively high intensity with a clear border of the internal capsule could be
potential diagnostic biomarkers for WD.Acknowledgements
We thank the Siemens Healthineers for providing technical support. We also thank all participants for their cooperation.References
1. Dusek
P, Bahn E, Litwin T, et al. Brain iron accumulation in Wilson disease: a post
mortem 7 Tesla MRI - histopathological study. Neuropathol Appl Neurobiol.
2017;43(6):514-532. doi:10.1111/nan.12341
2. Yuan XZ, Li GY, Chen JL, Li JQ, Wang XP.
Paramagnetic Metal Accumulation in the Deep Gray Matter Nuclei Is Associated
With Neurodegeneration in Wilson's Disease. Front Neurosci. 2020;14:573633.
doi:10.3389/fnins.2020.573633