Katrina Milbocker1, Eric Brengel1, Gillian LeBlanc1, and Anna Klintsova1
1Psychological & Brain Sciences, University of Delaware, NEWARK, DE, United States
Synopsis
Fetal Alcohol Spectrum Disorders (FASD)
is an umbrella term used to identify individuals with a history of prenatal
alcohol exposure which results in a spectrum of diagnostic disorders. 1 in
20 infants born in the U.S. has been diagnosed with an FASD, creating a major
public health crisis. Deficits in corpus callosum myelination resulting from
prenatal alcohol exposure have been correlated with impairments to perceptual
learning and executive function in adolescents diagnosed with FASD. This
study investigates the therapeutic potential of an exercise
intervention to ameliorate alcohol-induced damage to corpus callosum
myelination in a rodent model of FASD.
Introduction
Fetal Alcohol Spectrum Disorders (FASD)
resulting from prenatal alcohol exposure have a high prevalence around the
globe, with 1 in 20 live births in the U.S. being affected annually1.
Adolescents affected by FASD exhibit reductions in myelination to the corpus
callosum, a major commissural white matter tract, and this structural anomaly
is correlated with several cognitive and behavioral deficits including impairments
to perceptual reasoning and executive function2.
CNS myelination is imperative for
optimizing neuronal signaling. A unique feature of myelination in the mammalian
brain is that it begins during the brain growth spurt and continues to be
modified through adolescence to refine brain circuitry that will support
cognitive processing in adulthood3. As a result, the trajectory of
myelination is easily influenced by early-life and adolescent experiences such
as exercise, social interaction, and learning4. Thus, we
hypothesized that exposure to a myelination-stimulating exercise intervention
in adolescence might mitigate the impact of prenatal alcohol exposure on corpus
callosum development. Previously, the Klintsova lab has demonstrated that
increased aerobic activity in adolescence or adulthood restores neuroplasticity
in a well-established rat model of FASD5.Methods
This study employs in vivo diffusion tensor imaging (DTI) scanning to investigate the
effects of: 1) neonatal alcohol exposure and 2) an adolescent exercise
intervention on corpus callosum myelination in a rodent model of FASD. Male and
female Long-Evans rat pups were alcohol-exposed via intragastric intubation
(5.25 g/kg/day) on postnatal days (PD) 4-9, targeting the rodent brain growth
spurt. Sham-intubated controls received no liquid during intubation. From PD
30-42 in adolescence, half of all rats had free access to a running wheel for
aerobic intervention. DTI scans were acquired twice longitudinally (on PD 30
and 42) in all rats using a 9.4T Bruker Biospec scanner to assess alterations
to corpus callosum myelination noninvasively. Corpus callosum was divided into
three sub-regions for analysis: the interhemispheric region (ICC) and the left
and right cortically-projecting region (LPCC and RPCC). Voxel size is 250µm x
250µm x 500µm and scans from the entire brain were captured resulting in a
total scan time of half an hour per session. Results
Analysis using mixed repeated measures
ANOVAs (within-subjects variable: weight/day; between-subjects variables:
postnatal treatment and intervention groups; covariate: sex) indicate that
fractional anisotropy increases in ICC (F1, 74 = 38.2, p < .001) and left/right PCC (F1,
75 = 17.6, p < .001; F1,
75 = 22.8, p < .001) across
development, as expected. Further, analysis with two and three-way ANOVAs
(postnatal treatment x sex; postnatal treatment x intervention exposure with
sex as a covariate, respectively) for data collected on PD 30 and 42 show that
fractional anisotropy values are lower in alcohol-exposed rats compared to
control rats at both time points (p
< .001), indicating that water is moving more freely within corpus callosum
in adolescent rats with previous alcohol exposure.
Analysis using mixed repeated measures
ANOVAs (within-subjects variable: weight/day; between-subjects variables: postnatal
treatment and intervention groups; covariate: sex) indicates that radial
diffusivity decreases in ICC (F1, 74 = 84.7, p < .001) and left/right PCC (F1, 75 = 17.5, p < .001; F1, 75 = 26.0, p < .001) across adolescence, as
expected with increasing myelination. However, analyses using two and three-way
ANOVAs (postnatal treatment x sex; postnatal treatment x intervention exposure
with sex as a covariate, respectively) for data collected on PD 30 or 42 show
that radial diffusivity values remain higher in alcohol-exposed compared to control
rats in all corpus callosum sub-regions (p
< .001). These findings suggest that alcohol exposure during the brain
growth spurt reduces corpus callosum myelination in adolescence.Discussion
As expected, we show that
age has an opposite but significant effect on the fractional anisotropy and
radial diffusivity across adolescence in all sub-regions of corpus callosum,
indicating that the white matter is developing in all animals in this study.
There was no significant interaction with exercise intervention. However,
cross-sectional analysis at each time point confirms that fractional anisotropy
and radial diffusivity values in all corpus callosum sub-regions are reduced in
AE compared to control rats. Importantly, when we compare values of anisotropy and
diffusivity in the AE brain on PD 42 to those from the control brain on PD 30,
we do not see any significant differences. Taken together, we interpret these
data to show that AE during the brain growth alters the trajectory of
myelination in corpus callosum leading to an observed hypomyelination in
adolescence when compared to controls. This is in line with numerous clinical
observations of white matter development in teenagers with FASD.Conclusion
Future research is needed to investigate if AE leads to a
persistent loss of myelination in CC in adulthood. More importantly, to
comprehensively examine the immediate and long-term effects of exercise on
myelination, future studies will include histological analysis of brain tissue
samples collected from these rats. Finally, increasing the length of the study
in an effort to examine the lasting effects of exercise on myelination in the
adult brain is warranted.Acknowledgements
The authors would like to acknowledge our funding sources: NIH/NIAAA R01AA027269 and UD COBRE: MRI Pilot Funding 2P20GM103653-06 awarded to Dr. Anna Klintsova. References
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