Julia Stabinska1,2,3, Helge Jörn Zöllner1,2, Hans-Jörg Wittsack3, and Alexandra Ljimani3
1F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States, 2The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United States, 3Department of Diagnostic and Interventional Radiology, Heinrich Heine University Dusseldorf, Dusseldorf, Germany
Synopsis
Recent studies have shown that a
three-compartment model is preferable when analyzing renal DWI signal, as the
conventional bi-exponential IVIM fitting does not differentiate between pure
water diffusion and incoherent intra-tubular fluid motion.
However, triexponential IVIM modelling in the kidney is challenging due to
suboptimal SNR of diffusion-weighted images. In the present study, we applied
Image Downsampling Expedited Adaptive Least-squared (IDEAL) fitting,
which utilizes image downsampling to generate high SNR images to
iteratively update the initial model parameters until the final image
resolution is reached, and achieved more reliable estimates of the IVIM-related
parameters compared with the conventional fitting methods.
Introduction
Conventional intravoxel incoherent motion
(IVIM) analysis of diffusion-weighted signal utilizes a bi-exponential decay
model to distinguish between capillary perfusion and tissue diffusion1.
However, more recent studies have demonstrated that a three-compartment model
might be preferable when performing DWI of the kidney2,3. It has
been speculated that incoherent renal water motion is linked to three sources:
(i) pure water diffusion in the renal tissue (ii) incoherent intra-tubular
fluid motion, and (iii) incoherent blood motion, associated with the slow,
intermediate and fast (pseudo-) diffusion components, respectively2.
Triexponential IVIM modelling in the
kidney remains challenging due to a limited b-value sampling and an inherently
low SNR of diffusion-weighted images2. Its performance is also
highly dependent on initial parameters and lower and upper parameter limits
used for the constrained nonlinear data fitting. Fixing either one or two
pseudo-diffusion coefficients is a common approach to improve fit stability,
but it may introduce bias to the perfusion fraction2.
In this study, we applied an Image
Downsampling Expedited Adaptive Least-squared (IDEAL) fitting algorithm that utilizes
the high SNR of downsampled images for iterative tri-exponential fitting to
improve the reliability of the of tri-exponential IVIM modelling of the human
kidney.Methods
Seven healthy
volunteers (5
females and 2 males, mean age 30.1±5.4 years) were recruited for this study.
DWI was performed on a 3T MRI system (MAGNETOM Prisma, Siemens Healthcare, Erlangen,
Germany) using a respiratory-triggered single-shot DW-EPI sequence with the
following parameters: 3 slices; TE/TR = 71/1900 ms; matrix: 176 x 176; voxel
size: 2.1 x 2.1 x 5.0 mm3; averages: 3; diffusion directions: 3; 16 b-values
distributed nonuniformly between 0 and 750 s/mm2.
Image
registration based on a normalized mutual information similarity measure was
applied to correct for spatial misalignment of DW-images. Data analysis was
performed using custom-written MATLAB routines. All 16 b-values were used to fit
a triexponential model to the DW signal in each voxel for estimating the S0
image and IVIM parameters (Dslow, Dinterm, Dfast,
finterm, and ffast). Three different fitting approaches
were tested: (i) a conventional voxel-wise fit (Voxel), (ii) an
optimized fit with fixed pseudodiffusion coefficients (Fixed D*)2,
and (iii) an iterative IDEAL fit (IDEAL)5. The flow chart of
the IDEAL fitting procedure is displayed in Figure 1. The DW images were
downsampled to: 1x1, 2x2, 4x4, 8x8, 16x16, 32x32, 64x64, 96x96, 128x128,
152x152, till the original size of 176x176. The initial parameters of each
voxel were determined by spatially interpolating the fitted parameters of the previous
downsampled images. The lower and upper bounds were tightly constrained to 20% of the initial values for
amplitude and 5% for the corresponding diffusion
coefficients. Mean
values and standard deviation of the cortical and medullary IVIM parameters and
coefficient of variation (CV) within the ROIs in the fitted parameter maps were
calculated.Results
Compared with the voxel-wise and optimized
fitting with fixed D*, the IDEAL method yields less noisy estimates of the f
parameters, allowing better visualization of the complementary renal
structures. A similar pattern was observed in all the imaged kidneys, in which
the highest values of fslow were found in the medulla, finterm
mostly corresponded to the cortex, renal columns and pelvis, and highest ffast
was measured in the renal arteries and veins (Figure 2).
The distribution of f and Dslow
parameter values obtained in the cortex and medulla of the right kidney shows substantial
reduction in variability for all ROIs with the IDEAL fitting compared with the
voxel-wise fitting. While Fixed D* shows similarly low variance in fslow,
finterm and Dslow estimates, it seems to slightly
underestimate the fslow and Dslow parameters when
compared with the remaining methods (Figure 3A and 3B).
The coefficients of variance of the fitted
cortical and medullary signal fractions and diffusion coefficient Dslow
were substantially lower when IDEAL fitting was applied (Figure 4).Discussion
The IDEAL fitting method quantifies IVIM-related parameters
based on starting values from tri-exponential fitting of iteratively
downsampled images until quantitative IVIM parameters maps of original
resolution are calculated. This approach provides accurate estimates of
the IVIM parameters that are consistent with literature without signal averaging
over all subjects or fixing any parameters2,3,4. This may be of
particular importance when applying IVIM analysis to study renal pathologies in
which substantial alterations in the physicochemical properties of blood and
tissue are expected.
The IDEAL algorithm uses tightly
constrained lower and upper bounds after initial fitting, and is therefore less
affected by low SNR than the conventional voxel-wise fitting
method. As a result, the IVIM parameter maps obtained by the IDEAL algorithm
show reduced coefficients of variation and higher contrast to noise ratio,
which are essential for reliable detection and characterization of pathologies
superimposed with normal renal tissue.Conclusion
The
IDEAL method improves the IVIM analysis using a tri-exponential model, allowing
better visualization of the renal structures and more reliable estimates of the
IVIM parameters than the conventional fitting methods.Acknowledgements
No acknowledgement found.References
- Le Bihan D, Breton E, Lallemand D, Aubin ML, Vignaud J,
Laval-Jeantet M. Separation of diffusion and perfusion in intravoxel incoherent
motion MR imaging. Radiology. 1988 Aug;168(2):497-505.
- van Baalen S, Leemans A, Dik P,
Lilien MR, Ten Haken B, Froeling M. Intravoxel
incoherent motion modeling in the kidneys: comparison of mono-, bi-, and
triexponential fit. J Magn Reson Imaging 2017;46:228-39.
- van der Bel R, Gurney-Champion OJ, Froeling M, Stroes ESG,
Nederveen AJ, Krediet CTP. A tri-exponential model for intravoxel incoherent
motion analysis of the human kidney: in silico and during pharmacological renal
perfusion modulation. Eur J Radiol 2017;91:168-74.
- Stabinska J, Ljimani A, Zöllner HJ, Wilken E, Benkert T, Limberg
J et al. Spectral diffusion analysis of kidney
intravoxel incoherent motion MRI in healthy volunteers and patients with renal
pathologies. Magn Reson Med. 2021 Jun;85(6):3085-3095.
- Zhou IY, Wang E, Cheung JS, Zhang
X, Fulci G, Sun PZ. Quantitative chemical exchange saturation transfer (CEST)
MRI of glioma using Image Downsampling Expedited Adaptive Least-squares (IDEAL)
fitting. Sci Rep. 2017 Mar 7;7(1):84.