Filip Klimeš1,2, Andreas Voskrebenzev1,2, Marcel Gutberlet1,2, Cristian Crisosto1,2, Robert Grimm3, Frank Wacker1,2, and Jens Vogel-Claussen1,2
1Institute of Diagnostic and Interventional Radiology, Hannover Medical School, Hannover, Germany, 2Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Centre for Lung Research (DZL), Hannover, Germany, 3MR Application Predevelopment, Siemens Healthcare GmbH, Erlangen, Germany
Synopsis
Demonstration of a high repeatability is a mandatory
step prior to the usage of new methods in clinical routine. Good to moderate
repeatability of 3D phase-resolved functional lung (PREFUL) MRI was shown
before. In this study, group-oriented registration (GOREG) was compared to
stepwise registration regarding the repeatability of 3D PREFUL ventilation
parameters in 30 study participants. Furthermore, image sharpness and
structural similarity (SSIM) were assessed. An increased repeatability of
dynamic 3D PREFUL ventilation parameters was observed using the GOREG approach.
Also, image sharpness and SSIM values were significantly higher for
measurements assessed with GOREG registration.
Introduction
Proton magnetic resonance 3D
phase-resolved functional lung (PREFUL)1 and similar techniques2,3 provide contrast-free ventilation-weighted whole
lung imaging by analyzing the signal of co-registered dynamic data of the lung
during acquired during free breathing. Registration of lung images is
challenging due to large deformations. As a solution, a stepwise registration
or group-oriented image registration (GOREG)4 might help to avoid the need for large
deformations between lung images and improve the registration accuracy. Recently,
a good to moderate repeatability of 3D PREFUL ventilation parameters in healthy
volunteers and chronic obstructive pulmonary disease (COPD) patients was
reported using a stepwise registration to an inspiratory respiratory position5. In
this study, our objective was to assess the influence of the GOREG and stepwise
registration scheme on repeatability and image quality of 3D PREFUL ventilation
parameters.Methods
20 healthy volunteers and 10 patients
with COPD underwent MRI at 1.5T (MAGNETOM Avanto, Siemens Healthcare, Erlangen,
Germany). Images
were obtained during approximately 8 minute of free-breathing with a prototypical
3D stack-of-stars spoiled-gradient-echo sequence with golden-angle increment
and following sequence parameters: FOV 50 x 50 cm2, TE/TR 0.81/1.9 msec, flip angle 3.5°, bandwidth 1500 Hz/pixel, matrix size 128 x 128, slice
thickness 4 mm, final upsampled isotropical resolution 2 mm3. The acquisition
was repeated for healthy volunteers after a break outside the scanner, and in
median within 7 days [6-8 days] for COPD patients.
After successful image reconstruction,
registration towards the inspiratory image using the stepwise and GOREG
registration, implemented in Advanced Normalization Tools (ANTs)6, was performed.
The stepwise
registration of approximately 40 phases was implemented, so that
deformation fields are calculated for neighboring respiratory states in
direction to end-inspiration (i.e. the 20th respiratory phase).
Then, the deformation fields are used consecutively to register all images,
e.g. for 1st respiratory phase, 19 deformations fields are applied
in order to register 1st respiratory phase on 20th respiratory
phase.
For the GOREG
registration, the procedure is complemented by an additional registration step:
intragroup registration. In the intragroup registration all images from one
group are registered on reference image of the corresponding group. The
averaged images of all groups are used for step-wise intergroup registration as
described above. From the registered data, a
regional ventilation (RVent) dynamic map with 16 respiratory phases was
computed as previously described7.
The RVent dynamics was analyzed by computing the regional flow-volume loops (FVL).
Similarity of all FVLs to a healthy reference FVL, determined by RVent
thresholding8,
is evaluated using cross-correlation (CC). Ventilation defect percentage (VDP)
maps were generated for RVent and CC (VDPRVent and VDPCC)
using published thresholds1,5,9.
Further, the RVent dynamics was assessed by generating ventilation time-to-peak
(VTTP) maps in % deviation from the expected peak at full inspiration (50%):
$$VTTP_{Dev} = \:\mid VTTP - 50\%\: \mid\cdot$$
Differences were
assessed with Wilcoxon signed rank tests and Pearson correlation analysis.
Repeatability analysis included coefficient of variation (CoV),
intraclass-correlation coefficient (ICC) and Bland-Altman plots. Furthermore,
the image sharpness of RVent maps and mean structural similarity index (SSIM)
of registered morphological images were computed.Results
The median computing time was significantly
different (P < 0.0001) between stepwise and GOREG approach (113 vs.
151 minutes per subject).
Significant correlations (r
range: 0.827-0.999, all P < 0.0001, Table 1) were observed between
ventilation parameters, image sharpness and SSIM indices derived using both registration
approaches in 1st and 2nd measurement comparison. This
resulted in systematically higher RVvent values, increased CC values with GOREG
registration as well as in significant VDP differences (Table 1).
Image
sharpness values and SSIM indices were significantly increased for GOREG
registration in both 3D PREFUL measurements (all P < 0.0001, Table 1,
Figure 1).
Regarding repeatability, median CoV values of
CC, VTTP and VTTPDev parameters were significantly lower (all P
< 0.0254, Table 2) for GOREG registration when compared to stepwise
registation (see Table 2). Visual comparison of repeatability
evaluation of COPD patients for both registration schemes is seen in Figure 2
for CC parameter and in Figure 3 for the VTTP parameter.Discussion
Results of this work indicate that the repeatability of dynamic 3D
PREFUL ventilation is significantly improved using GOREG registration and that
the image sharpness of RVent maps is significantly increased.
Repeatability analysis showed significantly decreased
median CoV values and significantly increased ICC values of dynamic ventilation
parameters (CC, VTTP and VTTPDev) for the GOREG registration scheme,
when compared to stepwise registration. This fact suggests that the GOREG
registration improves the accuracy of registration and repeatability of dynamic
3D PREFUL ventilation parameters. This can be explained by the increased
parenchymal signal due to averaging several slices within each breathing group
resulting in more accurate intergroup registration. Importantly, GOREG leads to
systematically higher RVent ventilation values and higher VDPRVent
likely due to the improved image quality compared to stepwise registration.
Similarly to previous 2D results4, the image
sharpness of RVent ventilation maps and also SSIM index of GOREG registered
morphological images was superior compared to stepwise registration. The drawback of GOREG
registration is a longer computation time (~38 minutes per subject).Conclusion
The GOREG
registration approach improves the repeatability of dynamic 3D PREFUL
ventilation parameters and results in superior image sharpness of RVent maps in
comparison to stepwise registration.Acknowledgements
This work was supported by the German Centre for Lung Research (DZL). The authors thank Melanie Pfeifer and Frank Schröder for outstanding technical assistance in performing the MRI examinations.References
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