Arterial spin labeling (ASL) is emerging as a valuable tool for various clinical applications primarily in the brain, but also in the body. In this session, we will review key clinical applications, including stroke, cerebrovascular disease, neurodegeneration, neuro-oncology as well as renal applications. We will highlight how both cerebral blood flow as well as transit time effects can be used in different clinical applications. Lastly, we will highlight challenges and recent initiatives to increase adoption of ASL perfusion imaging in the clinic.