Jiahui Li1, Prachi Singh2, Marzanna Obrzut1, Xin Lu1, Kevin J. Glaser1, Alina Allen3, Sudhakar K. Venkatesh1, Taofic Mounajjed4, Jun Chen1, Armando Manduca1, Vijay Shah3, Richard L. Ehman1, and Meng Yin1
1Radiology, Mayo Clinic, Rochester, MN, United States, 2Sleep and Cardiometabolic Health, Pennington Biomedical Research Center, Baton Rouge, LA, United States, 3Gastroenterology, Mayo Clinic, Rochester, MN, United States, 4Anatomic Pathology, Mayo Clinic, Rochester, MN, United States
Synopsis
Multiparametric MRI and MRE was performed in 27 obese patients
who had biopsies of liver and
subcutaneous adipose tissues. We found significant correlations between the
mechanical properties of liver and subcutaneous fat and their histological and
biochemistry results. A model combining liver proton density fat fraction and subcutaneous
fat stiffness had a slightly higher AUC for diagnosing nonalcoholic
steatohepatitis than liver stiffness (AUC: 0.87 vs. 0.84, p=0.74). The results indicate
that obesity-induced systemic inflammation affects both adipose and liver
tissue mechanical properties and, therefore, models utilizing mechanical biomarkers
from adipose tissue may improve the diagnosis of steatohepatitis in obese
patients.
Introduction
The
excessive accumulation of fat in obesity contributes to the development of systemic
metabolic disorders, like type 2 diabetes, metabolic associated fatty liver
disease (MAFLD, previously called nonalcoholic fatty liver disease or NAFLD),
and nonalcoholic steatohepatitis (NASH) (1, 2).
Adipose tissue plays an important role in the pathophysiology of obesity and
the associated MAFLD progression. When the capacity of adipose tissue to store
excessive energy is diminished, the hepatocytes will hold the spillover of
lipids, leading to MAFLD. These metabolic alterations result in systemic
inflammation, which promotes the development of NASH (3). It has been demonstrated that adipose tissue inflammation is established before the development of
hepatic inflammation (1), and the tumor
necrosis factor (TNF) is overexpressed in subcutaneous adipose tissue in
obese patients (4). Since many studies
have demonstrated that liver MRI/MRE can distinguish and diagnose NASH (5, 6), the objective of this study is to
investigate the relationship between adipose and liver tissue mechanical
properties. This pilot study also explored the potential for using a
comprehensive abdominal MRE assessment of both liver and subcutaneous fat to diagnose
NASH in obese patients.Methods
All activities related to
human subjects were reviewed and approved by our institutional review board. 27 obese patients (BMI: 45 ± 7kg/m2; morbidly obese: 23/27; female: 23/27; age:
47 ± 10y) were enrolled in this
study. All of them had liver and subcutaneous fat biopsies within three months
of the MRI/MRE exams (5). Multiparametric 3D MRE (30Hz and 60Hz) and 6-point
Dixon MRI were performed on 1.5T whole‐body scanners (GE Healthcare, Milwaukee,
WI). The shear stiffness (SS) and loss modulus (LM) of subcutaneous fat and
liver were calculated from manually drawn ROIs in the MRE image for both
frequencies (Figure 1). In subcutaneous adipose tissue, the ROIs were selected
in the regions with sufficient magnitude and wave signal. We excluded the areas
with preload concerns, like the back and area beneath the MRE passive pneumatic
actuator. mRNA and protein expression of components of the renin-angiotensin
pathway were analyzed. Further, mRNA expression of key inflammatory and
oxidative stress markers were also determined, including angiotensin II type 2
receptor (AT2R), angiotensinogen (AGT), and tumor necrosis factor (TNF). Spearman’s
correlations were used to analyze the relationships between the MRI/MRE
measurements of liver and subcutaneous fat and the histological/biochemistry
results. Fisher’s exact test was used to compare the difference between contingency
variables. A nominal logistic fit model was used to predict NASH diagnosis. All statistical analyses were performed using
JMP Pro version 14.1.0. A significance level of 0.05 was used.Results
The
mechanical properties of both liver and subcutaneous fat (SS and LM) had
significant correlations with the biochemistry characteristics of subcutaneous
fat (Table 1). Subcutaneous fat with higher mRNA expression of TNF showed
significantly higher shear stiffness and loss modulus (SS, p=0.03; LM,
p<0.01) (Figure 2A.B.). The Mosaic plot in Figure 2C shows fewer NASH
patients in the high TNF group (p<0.01). The nominal logistic fit models all
predicted the diagnosis of NASH well, and there is no significant difference
between models (Model 1 vs. Model 2: p=0.74; Model 2 vs. Model 3: p=0.39; Model
1 vs. Model 3: p=0.12) (Figure 3). Discussion
The significant correlations between the measured mechanical properties
of subcutaneous fat and the mRNA expression of TNF in subcutaneous fat indicate
that MRE-based biomarkers from adipose tissue reflect metabolic dysfunction in
obese patients. Bullo et al. found that TNF is overexpressed in subcutaneous
fat in obese patients when compared with healthy volunteers and they believe
that the TNF system may be a homeostatic mechanism that prevents further fat
deposition (7). In this study, we found
that in patients who had higher mRNA expression of TNF, fewer were diagnosed with
NASH. This supports their hypothesis that the high expression of TNF limited
the fat deposition. Thus, simple steatosis progression to NASH might be halted
or alleviated with high TNF.
The prediction model composed of liver stiffness
and fat fraction has been used for diagnosing NASH (5) and evaluating treatment efficacy after bariatric surgery in
obese patients (6). When replacing liver
stiffness with subcutaneous fat stiffness in the prediction model, we did not
observe a significant difference in the accuracy between the two models. If all
three parameters are combined, the diagnostic accuracy is superior to both
two-parameter models. This finding reinforces the clinical consensus that adipose
tissue plays an important role in the disease progression of NASH in obese
patients (3). Using a combination of
liver fat fraction, liver stiffness, and subcutaneous fat stiffness, this
prediction model could comprehensively characterize the systemic metabolic
disorders of NASH. Conclusion
The MRE measurements of subcutaneous adipose tissue may
reflect the pathophysiologic
influence of fat in obesity and metabolic associated fatty liver disease. A comprehensive
assessment of liver fat fraction, liver stiffness, and subcutaneous fat
stiffness can improve NASH diagnosis and distinguish metabolic dysfunctions systematically.Acknowledgements
This study is funded by NIH grants EB017197 (M.Y.), EB001981(R.L.E.),
DK115594 (A.M.A.), DK059615 (V.S.), and DoD grant W81XWH-19-1-0583-01 (M.Y.).References
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