Eloïse Mougel1, Sophie Malaquin1, Melissa Vincent1, and Julien Valette1
1Université Paris-Saclay, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Molecular Imaging Research Center (MIRCen), Laboratoire des Maladies Neurodégénératives, Fontenay aux Roses, France
Synopsis
Brain
lactate compartmentation is an important but debated neuroscience question. By
assessing local microstructure where lactate is diffusing in,
diffusion-weighted MRS has unique potential to non-invasively assess lactate
compartmentation. We propose to increase lactate signal using selective pulses
(SP) to cancel J-modulation.
We compare lactate signal behavior in diffusion-weighted experiments performed
in vivo, using either spin echo or stimulated echo sequence relying on
selective pulses.
We verify here that the signal increases in both cases, compared to
conventional cases using broad pulses. Spin echo using SP appears the most
valuable option to measure lactate diffusion at high b-values.
INTRODUCTION
Non-invasive
assessment of lactate content inside the different cellular compartments in the
brain would be invaluable to investigate energy metabolism and
astrocyte-to-neuron lactate shuttle hypothesis1,2. Diffusion-weighted
magnetic resonance spectroscopy (DW-MRS) may provide information about the
microstructural environment of lactate, and thus allow assessing in which
cellular compartment it is diffusing3. Nevertheless, lactate diffusion
measurements are made extremely difficult by low lactate’s signal-to-noise
ratio, especially when the diffusion-weighting (b) increases.
We propose
to apply selective pulses (SP) to increase the signal of the CH3 lactate peak
at 1.3 ppm by cancelling the effect of scalar coupling (J) with the CH lactate
peak at 4.1 ppm. We demonstrate experimentally the signal gain that this method
can yield, and evaluate its benefits for two classes of sequences used in
DW-MRS: spin echo (SE) sequence and stimulated echo (STE) sequence4.METHODS
We
investigated sequence performances with two-by-two comparisons: diffusion-weighting
STE using broadband pulses followed by an adiabatic LASER localization module
(STE-LASER5), as classically used in our lab (Fig. 1A), versus its selective STE counterpart
(Fig. 1B); broadband versus selective
diffusion-weighting spin echo sequences (Fig. 1C and 1D); and broadband STE versus selective SE sequence. For SE,
excitation was achieved with AHP pulse and refocusing was achieved with either a
0.1-ms broad pulse or a 5-ms selective homemade π pulses. STE consisted
in either three 0.1-ms broad pulses or three 9-ms $$$\frac{\pi}{2}$$$ SP. SP were designed to
select the [4.1; 0.8] ppm range, using Shinnar-Le Roux (SLR) algorithm6.
Note that, while a VAPOR water suppression module was required when using broad
pulses, sequences using partially selective pulses did not require water
suppression. Figure 1E lists main sequence parameters.
Three
anesthetized C57/BL6 mice were scanned in a Bruker Scanner at 11.7 T equipped
with a cryoprobe. Isoflurane level ranged from 1 % to 1.3 % between animals,
but was kept constant throughout the experiment for each animal. Diffusion-weighted
experiments were performed in four blocks of 32 averages for five different b
(0.02, 3.02, 8, 15, 20 ms/µm2) to measure the diffusion attenuation of metabolites
(Lac, NAA, myo-inositol Ins, total creatine tCr and choline compounds tCho). Spectra
were acquired in a volume of 31.5 mm3 centered around the hippocampus, and analyzed
with LCModel7. Experimental MM spectra were included in LCModel
basis-sets. ADC was computed using the two lowest b-values (0.02 and 3.02
ms/µm2). RESULTS
STE
comparison demonstrates the selectivity of $$$\frac{\pi}{2}$$$ SP pulses over the [4; 0.5] ppm range (Fig. 2A-B). CH3 lactate peak at 1.3 ppm is higher by ~40% in the
selective case (Fig. 2C-D). Selective STE generally appears slightly more precise
than non-selective STE when looking at S/S0 (Fig. 2E-F). However, ADC precision
is very similar in both cases (Fig. 2G).
In the SE
case (Fig. 3), selection is effective in almost the same range than in the STE
case. CH3 peak at 1.3 ppm is totally in-phase in the selective case, due to
suppressed J-modulation. Measurement with SE SP is more precise (i.e.
coefficient of variation of S/S0 is smaller) than with SE BP: (Fig. 3E). In addition, SE SP ADC is more precise
than in other SE case for lactate and myo-inositol.
Finally,
comparing selective SE to conventional STE BP, we do not observe a large rise
of CH3 lactate peak at b = 0.02 ms/µm2 (Fig. 4C-D). Nevertheless, measurement
precision with SE SP remains higher than in the STE case. From SE SP data,
calculated ADC is much more precise than the one obtained from STE BP data.DISCUSSION
Canceling
J-modulation provides signal gain for the CH3 lactate peak at 1.3 ppm, which is
particularly striking when comparing the two SE sequences. For the STE
sequences, signal gain is mitigated by the long duration of SP pulses, which requires
increased TE (3 times longer).
Frequency
profile of the 5-ms SP π pulse is slightly worse than that of the 9-ms SP $$$\frac{\pi}{2}$$$ pulse. The transition band begins around 4.1 ppm (Fig. 5B), so some residual
J-coupling still occurs for lactate, which can be worsened by B1 inhomogeneity.
However, SE
SP still provides the best measurement precision for lactate and other
metabolites, even at high b. ADC is also evaluated more precisely with this
sequence. Hence, SE SP has significant potential for diffusion measurement due
to increased lactate signal, while still retaining signal for a large number of
other metabolites at the same time (unlike polychromatic pulses that could also
increase lactate signal at 1.3 ppm but would suppress most other metabolite
resonances9).CONCLUSION
Using selective pulses allows increasing lactate signal while retaining most spectral
information about other metabolites. In the end, spin echo using SP seems the
best option to precisely measure lactate diffusion at high b-values. This
method could be very useful to perform DW-MRS to assess lactate
compartmentation in brain.Acknowledgements
This
project has received funding from the European Research Council (ERC) under the
European Union’s Horizon 2020 research and innovation programmes (grant
agreement No 818266).References
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