Chemical exchange saturation transfer (CEST) is an MRI technique that is capable of enhancing the MRI sensitivity of low concentration metabolites and proteins in vivo. This course will cover the recent advances of the CEST technique, including the origin of in vivo CEST contrasts, the acquisition modes and the quantification methods. Based on these improvements, applications will be focused on observing several energy metabolites such as creatine, phosphocreatine and glucose in stroke, tumor and neurodegenerative diseases.