This lecture will cover cutting-edge preclinical brain tumor imaging methods. Topics covered are: fundamentals of preclinical MRI; brain tumor microenvironment (TME); structural and functional changes during brain tumor progression and the development of new preclinical techniques for brain tumor imaging. These new techniques include: magnetic resonance microscopy (MRM), resting-state fMRI (rs-FMRI), O2-MRI, amide proton transfer (APT) MRI, focused ultrasound (FUS) and MRI, magnetic resonance elastography (MRE), magnetic particle imaging (MPI), 19F MRI, and near-infrared imaging (NIR) and MRI. Recent techniques for immunotherapy and glymphatic imaging will also be covered along with a "peek into the future" of brain tumor imaging.