Fumiko Seki1,2, Seiji Shiozawa2, Sho Yoshimatsu2, Yuji Komaki1, Marin Nishio3, Erika Sasaki1, and Hideyuki Okano2
1Central Institute for Experimental Animals, Kawasaki, Japan, 2Department of Physiology, Keio University School of Medicine, Tokyo, Japan, 3Department of Radiological Science, Tokyo Metropolitan University, Tokyo, Japan
Synopsis
This study investigated whether MRI could detect brain abnormalities
in transgenic marmoset models of Alzheimer’s disease (AD). Magnetization
transfer (MT) contrast-MRI have a potential to detect the presence of amyloid plaques,
which could be present in the early stage of the diseases. Voxel-based analysis
was conducted to explore whether any difference existed between AD models and healthy
marmosets (young-to-middle-aged adults). Significant increase of MT-ratios
imaging was observed in posterior cingulate cortex whereas decrease was observed
in unilateral inferior temporal cortex. This result supported MT-imaging was
sensitive to early abnormalities. Continuous evaluation until old age is worthwhile
to be clinically relevant.
INTRODUCTION
Transgenic techniques have been the most commonly used
for the animal models of Alzheimer’s disease (AD). The reasons included evaluating
brain abnormalities before symptoms appeared, which allowed providing knowledge which human studies were
difficult to reach. The studies using AD disease transgenic mice reported
Magnetization Transfer Contrast MRI (MTC-MRI) were sensitive to track
amyloid plaque, which might be already present in the early stage of
disease12. However, the findings were inconsistent in that MT-Ratios decreased
in human studies3 bur increased in mouse studies. It would be possibly due to
the MT-Ratios increase in early stage of AD, but few studies examined whether
the species gap between rodents and humans might influence its discrepancy. To
address it, this study applied
AD transgenic models of (?) the common marmosets (marmosets), non-human
primates. Longitudinal MTC-MRI was
performed to the marmosets AD models at the age from young-middle-aged adult to
detect brain abnormalities in early onset. METHODS
<Marmosets>
We produced the transgenic marmosets overexpressing the Swedish
mutant of human APP, which we evaluated as the AD model (AD model, N=2, both
were female).
MRI was performed at the age of 3.5 years. We repeated the scan
per year until 5.5/6.5 years old, 3 times in total (i.e., 3.5, 4.5, and 5.5
years old). Control MRI data were acquired from adult healthy marmosets (N=30)
ranged from 2 to11 years old (mean age = 5.7 years old, female N=15, male
N=15). In general, marmosets reach adulthood by 2 years old and 8
years old is considered as aged4.
<Image acquisition>
T1-weighted images (T1WI) and MTC were acquired using 7.0 T
Biospec 70/16 scanner (Bruker BioSpin:Ettlingen, Germany) with following
parameters (T1WI: repetition time/TR, 6000 millisecond/ms; echo time/TE, 35 ms;
inversionTime ; 1300 ms. image matrix
192 x 192 x 120. MT–Ratios: FLASH with/without an off-resonance frequency set
bandwidth (bandwidth: 300 Hz, off-set frequency: 1500 Hz) TR: 2600 ms, TE: 2.86
ms, flip angle 70°, image matrix 128 x 128 x 54).
<Image analysis>
For conducting voxel-based analysis, T1WI and MT-Ratios images were
preprocessed using statistical parametric mapping (SPM12) running on Matlab.
After preprocessing using DARTEL5, a two-sample t-test was performed to compare
the difference between disease models and control. We repeated the statistical analysis as we acquired the AD model data per
year using the same control data.RESULTS
Voxel-based morphometry (VBM) using T1WI showed no significant
decrease in volume at any time point, whereas significant volume increase was
observed at 3.5 years old at the right hippocampus, which was not observed
thereafter.
On the other hand, significant MT-Ratio decrease was observed
for the one subject in the left side of the inferior-temporal cortex at any time-points
(3.5,4.5,5.5 years old). There was also significant increase in the part of precuneus/posterior
cingulate cortex until 4.5 years old. Although significant increase was not
observed in precuneus/posterior cingulate cortex at the age of 5.5 years, the significant
increase was observed in multiple regions such
as the primary visual cortex and hippocampus.DISCUSSION
Voxel-based
analysis with MT-Ratio detected the regions showing significant differences existed
between control and AD disease models. The finding was relatively similar to
the previous report in mouse: MT-Ratio increase in the retrosplenial
cortex, while our analysis showed MT-Ratio increase in the left side of the posterior
cingulate cortex. The correlation between MT-Ratio and immunohistochemistry
indicated the region might contain high amyloid load1.
Our
analysis also showed MT-Ratios decrease was significant in the left side of
inferior-temporal cortex. Precious mouse study, in contrast, showed MT-Ratio decreased
in white matter, the corpus callosum splenium, but not at the gray matter2. The
marmoset result could be close to human in that human studies found decreased
MT-Ratio in gray matter. The difference lay in that the decreased MT-Ratio was
observed in the hippocampus and the lateral occipital cortex. Given AD patients
were not at the stage of early onset, it would be necessary to continue data
acquisition to compare with marmosets.
This
study was limited in that the number of AD model was small due to a limited
number can be produced. Nonetheless, MRI evaluation using marmoset AD model is necessary
to investigate to get the result the previous closer to the human studies. Further,
the correlation with immunohistochemistry would be worthwhile to valid the
results and to reveal the pathological profile of these models.CONCLUSION
This
study using AD model marmosets supported previous reports that MTC-MRI
can be the sensitive method to detect brain abnormalities before the symptom appears.
In this study, we took the exploratory approach to examine whether abnormal
regions existed in AD model. Detailed analysis of detected abnormal regions
would be coming tasks to examine which aspect might be normal by use of different
measurements such as resting-state fMRI.Acknowledgements
This research is supported by the program for Brain Mapping by Integrated Neurotechnologies for Disease Studies(Brain/MINDS) from Japan Agency for Medical Research and development, AMED. References
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