Xuan Niu1,2, Yingxiang Sun2, Shuoqiu Gan2, Ming Zhang2, and Lijun Bai3
1Department of Radiology, Washington University School of Medicine, St. Louis, MO 63110, St.Louis, MO, United States, 2Department of Medical Imaging, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China, Xi'an, China, 3The Key Laboratory of Biomedical Information Engineering, Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China, Xi'an, China
Synopsis
Post-traumatic
headache (PTH) is one of the most frequent and persistent physical symptoms
following mild traumatic brain injury (mTBI). However, the underlying
neurobiological basis and modulatory component remained unclear. Evidence indicated that neuroinflammation is a major contributor in the
pathogenesis of PTH. We hypothesized that the effect of peripheral
inflammatory signaling on PTH could be produced by influencing brain structure
that subserve pain modulatory function. Our findings demonstrated that neuroinflammation
following mTBI is a potential process affecting structural
changes in cognitive component of pain modulation, which may serve
as a potential neurobiological mechanism
underlying the emergence and persistence of PTH.
Introduction
Post-traumatic headache (PTH) is one of the most frequent and persistent physical symptoms following mild traumatic brain injury (mTBI). However, the underlying neurobiological basis and modulatory component remained unclear.1 Evidence indicated that neuroinflammation is a major contributor in the pathogenesis of PTH.2 We hypothesized that the effect of peripheral inflammatory signaling on PTH could be produced by influencing brain structure that subserve pain modulatory function.3 Peripheral
inflammatory markers can interact with multiple central pathways as a principal
channel for immune-to-brain communication in the development of pain states.2
This study aimed to test whether inflammatory processes affects PTH-specific
grey matter volume (GMV) changes leading to pain dysfunction in early mTBI.
Longitudinally, we also examined if these abnormalities can be persistent in
patients developing chronic headache 3 months post-injury. Methods
77
mTBI patients initially underwent neuropsychological, peripheral blood inflammatory
markers measurements and MRI scans within 7 days post-injury (T-1) and 54 (70.1%)
of patients follow-up at 3-month (T-2). 42 matched healthy controls completed
the same protocol at T-1 once. Voxel-based morphometry (VBM) analyses of GMV were
conducted in PTH patients, compared with non-PTH patients and healthy
volunteers at both initial and follow-up stages.Results
The mTBI patients
with acute APTH presented significantly increased brain volume, mainly in the default mode network (DMN), compared with other two control
groups. Among all the serum
biomarker assay, stepwise regression analysis found that the initial C-C motif
chemokine ligand 2 (CCL2) level significantly contributed to acute pain symptoms following
mTBI, which was mediated by rapid greater GMV change within the key DMN region (dorsal
posterior cingulate cortex, dPCC) in early mTBI. For the chronic stage, CCL2 level was
significantly unregulated only in the mTBI patients with chronic
PTH(CPTH),as well as GM volume
increase in a subset of regions (dPCC and dACC) showing significant GMV group
differences at the acute stage.Discussions and Conclusions
The
increased GM changes were presented in the acute PTH, whereas bi-directional
changes of GM were observed in the chronically persistent PTH. In the acute phase, the PCC structural
change was shown to be an important mediator contributing the effect of CCL2
level on the dysfunctional pain symptoms observed in mTBI
patients. Importantly, significant CCL2 rise over time and GM
volume alterations in dPCC were found in patients developing CPTH. This study
provide initial evidence that
neuroinflammation following mTBI is a potential process4,5 affecting
structural changes in cognitive component of pain modulation,6 which
may serve as a potential neurobiological
mechanism underlying the emergence and persistence of PTH in mTBI.7 Acknowledgements
We thank all patients and healthy volunteers who participated in the study. This
research was supported by the National Natural Science Foundation of China
[Grant Numbers 81571752, 81771914]. References
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