Ratthaporn Boonsuth1, Marco Battiston1, Francesco Grussu1,2, Marios C. Yiannakas1, Torben Schneider3, Rebecca S. Samson1, Ferran Prados1, and Claudia A. M. Gandini Wheeler-Kingshott1,4,5
1NMR research Unit, Queen Square MS Centre, Department of Neuroinflammation, UCL Queen Square Institute of Neurology, Faculty of Brain Sciences, University College London, London, United Kingdom, 2Centre for Medical Image Computing, Department of Computer Science, University College London, London, United Kingdom, 3Philips Healthcare, Guildford, Surrey, United Kingdom, 4Brain MRI 3T Research Centre, IRCCS Mondino Foundation, Pavia, Italy, 5Department of Brain and Behavioural Sciences, University of Pavia, Pavia, Italy
Synopsis
Major
MRI scanner upgrades are generally required to improve performance
and image quality; however, they can also potentially introduce systematic
changes in quantitative MRI (qMRI) metrics and affect their accuracy and
precision. To date the evaluation of the effect of scanner upgrades has
focussed mainly on volumetric measurements, whereas the effect on quantitative
parametric maps remains unexplored, especially when comparing analog and digital
signal pathways plus multiband. Here we report findings on
quantitative T1 to assess the potential effect of scanner
upgrades
on qMRI. We found negligible differences, suggesting that T1 measurements remain
stable following a major scanner upgrade.
INTRODUCTION
Quantitative
MRI (qMRI) methods can be used to characterise tissue organization and/or
composition in vivo non-invasively1.
Furthermore, given their quantitative nature, they are expected to be more reproducible
across hardware/software configurations and time points. An MRI scanner
upgrade typically consists of updating the control software as well as
substituting hardware components with updated technology. This process generally aims to
improve performance and image quality, while potentially introducing systematic
changes in qMRI metrics2,3,4. There are many concerns, including reproducibility of quantitative parameters, the introduction
of possible bias, reliability and standardization1,5 that need to be
considered in the context of multi-centre and/or longitudinal studies.
In this work, we aimed to characterise the
effect of a major scanner upgrades on quantitative matrices, while also taking advantage of
novelties for acceleration or increased resolution. The change consisted of software and hardware improvements from a 3T Philips Achieva system upgraded to a 3T Philips Ingenia CX, with
digital broadband architecture. We take the quantitative longitudinal
relaxation time T1 as a case study, since it represents a primary parameter in
MRI widely used as a surrogate marker of tissue integrity6, but aim
to present a more comprehensive set of qMRI metrics in the near future.METHODS
Participants: The same three
healthy controls (2 males, 1 female, age range 29-35) were scanned four times
pre- and four times post-upgrade.
MR
imaging:
Imaging was
performed with a 32-channel head receive-only coil on a 3T Philips MRI system
(Achieva on software release 3 upgraded to Ingenia CX on release 5 with
multiband technology7, after 9 years of operation). The 32-channel
coil for post-upgrade scanning was changed with the new configuration embedding
analog-to-digital conversion8. Pre- and post-upgrade protocols
consisted of multiple inversion time (TI) inversion recovery (IR) scans
using a multi-slice
single-shot spin echo-EPI sequence with non-spatially selective inversion pulse9.
Table 1 shows MRI parameters for pre- and post-upgrade, optimised independently
to take advantage of the upgrade.
Image analysis
Image registration
and quantification: IR images were corrected for EPI distortions with FSL
topup10,11 and then
warped to MNI space using NiftyReg reg_f3d12.
T1fitting: Non-linear
least-squares fitting with custom-written Python code provided T1voxel-wise maps.
Subject analysis: We evaluated distributions of T1 values within white matter (WM)
and grey matter (GM) of each subject, using regions-of-interest defined in MNI
space.
Voxel-wise
comparison: The system intra-
and inter- time-point reproducibility and potential bias between time-points
were evaluated,
using voxel-wise comparison within the same subject. We also calculated the linear correlation index (R) of T1 values
between pre- and post-upgrade and scattered on a voxel-by-voxel basis for all subjects.
Repeatability assessment: We quantified repeatability of
T1 both pre- and post-upgrade to test whether the upgrade positively improved
the precision of T1 measurements. We conducted the analysis in MNI space,
calculating a voxel-wise intraclass correlation coefficient (ICC)13 and percentage coefficient of variation
(%COV)14 using custom-written Python code based on NiPype15.
ICC measures the fraction of total variability due to biological differences
among subjects, while %COV provides an estimate of the amount of variability
with respect to the mean population value of the metric.RESULTS
T1 maps show known contrasts (i.e. CSF T1 >
GM T1 > WM T1) and are homogenous with little variation between pre- and
post-upgrade (Figure 1). The example of T1 distributions in WM and GM
are illustrated in Figure 2, where values from different scanning
sessions are reported along columns. Mean T1 values for all subjects
pre-upgrade were 879.42ms and 1436.50ms in WM and GM respectively, while
post-upgrade they were 862.83ms and 1407.42ms. The voxel-wise comparison resulted in a mean
and median difference in T1 estimates from pre- and
post-upgrade of 16.63ms and 11.16ms in WM, and of 29.06ms and 30.55ms in GM
respectively. Figure 3 shows a positive strong correlation of T1 values between pre-
and post-upgrade, with
linear correlation index of 0.61 in WM and 0.88 in GM. Table 2 shows stable reproducibility
figures for pre-and
post-upgrade, expressed as the ICC index and %COV.DISCUSSION AND CONCLUSION
In this study, we found that T1 values
post-upgrade are comparable to T1 values pre-upgrade when evaluated at the inter- and
intra-subject level. T1 measurements are reproducible across the scanner upgrade. We found negligible differences in quantitative T1 values obtained
pre- and post-upgrade, implying that T1 is a robust marker of WM integrity
along the life-time of an MRI scanner when major upgrades are likely to happen
at least once. The results
presented in this study show a strong positive correlation between pre- and
post-upgrade when compared on a voxel-by-voxel level. In order to assess repeatability, ICC and %COV
were analysed showing a comparable variability between pre and post-upgrade. This confirms that
T1 can be
measured with similar or
improved precision post-upgrade while enabling at the same time the use of faster sequences and increased
resolution. In the near future, we also plan to
expand the current evaluation and include additional qMRI metrics derived from
magnetisation transfer and diffusion imaging.Acknowledgements
Acknowledgement to the
UCL-UCLH Biomedical Research Centre for ongoing funding; the European Union’s
Horizon 2020 research and innovation programme under grant agreement No.
634541; the Engineering and Physical Sciences Research Council (EPSRC
EP/R006032/1, M020533/1); Spinal Research (UK), Wings for Life (Austria), Craig
H. Neilsen Foundation (USA) (jointly funding the INSPIRED study); Wings for
Life (#169111); the UK Multiple Sclerosis Society (grants 892/08 and 77/2017);
Guarantors of Brain. We thank Philips Healthcare for
assistance in protocol development and for access to research protocols.References
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