Karyn Elizabeth Chappell1, Djordje Brujic2, Mihailo Ristic2, Donald McRobbie3, Wladyslaw Gedroyc4, Catherine Van Der Straeten5, and Richard Meeson6
1Medicine, Orthopaedic Surgery, Stanford University, Redwood City, CA, United States, 2Mechanical Engineering, Imperial College London, London, United Kingdom, 3University of Adelaide, Adelaide, Australia, 4Imperial College London, London, United Kingdom, 5Imperial College London/Ghent University, Ghent, Belgium, 6Royal Veterinary College, London, United Kingdom
Synopsis
A proof of concept study in a spontaneous ligament
rupture dog model assessed if the magic angle effect disappears in unhealthy
tissue. Ten dogs were scanned using the
MADI technique. Standard deviation (SD) maps
of signal intensity were computed and measured in an interactive display. Three subjects showed abnormally high SD; two
at the ACL origin, one in the trochlear articular cartilage. Fibre orientation maps showed either
disorganised or highly organised collagen fibres in these subjects. On dissection two subjects had partially torn
ACLs and the other a fibrocartilage lesion. There was excellent correlation
between the MADI and dissection findings.
Introduction
It
has been suggested that the magic angle effect (MAE) will disappear in tissue
which is not healthy or when the collagen structure is disrupted1,2.
Chang et al. postulate that ligament tearing or significant degeneration
decreases tissue anisotropy which disrupts the contrast mechanism2. Evidence for this comes from several
non-physiological models that mimic degeneration, ageing and injury3,4,5,6. For this work a model for clinical cruciate
rupture with associated ligament pathology was sought. A small proof of concept study in dogs was performed
as the best alternative for an animal model to assess a spontaneous ligament
rupture. Dogs are prone to spontaneous
arthritis7 and frequently suffer ACL ruptures, secondary changes of
OA and meniscal injuries8. Methods
Ethical approval from the Royal Veterinary College (RVC)
was sought and approved (URN 2017 1659-3).
Any large breed dog (> 15kg) which were euthanized at the RVC and
whose owners indicated disposal by group cremation were included in the
study. During post-mortem examination
the limbs were harvested, numbered, sealed in Zip lock bags and cold stored at
4˚C. Harvested limbs were scanned within five days
using the MADI technique9.
Once scanned the limbs were frozen until they could be
dissected, photographed and a pathological diagnosis from a specialist
orthopaedic veterinarian performed.
Visual comparison using a standard deviation
(SD) map composed of signal intensity variations from nine 3DT1 FLASH volumes orientated differently to B0 was computed in Matlab 2015b. An interactive display was used to measure
the SD range and enable segmentation of the ACL and articular cartilage (AC). Signal intensities for each segmented voxel
were used to compute the net collagen orientation of the voxels. The results of
this process calculated fibre direction vectors for each voxel displayed as
fibre orientation maps (FOM). The vectors glyphs were also displayed in 3D
using ParaView 5.1.2. A unique
experimental number given to the dog knee during limb harvesting was used to
un-blind the dog knees so the results could be correlated.Results
During a 6 month data collection period ten knees were
harvested and scanned from five dogs (fig1).
The SD values were much higher in subjects 1,9 & 10. The high SD areas on subject 9 (fig2A) and
subject 10 (fig2B) corresponded to the origin of the ACL. The measured voxel SD
were much higher (0.85-0.92) than other voxels containing the ACL (0.4-0.6).
This indicated that there was a big variation of signal intensity over the
corresponding voxels in these particular areas. The third brighter voxel area
was identified on subject 1 (fig2C) and corresponded to the articular cartilage
between the trochlear grooves. Again the SD of the voxels in this area was much
higher (0.88) than the other voxels containing articular cartilage (0.4-0.55).
Subject 1’s AC in the trochlear groove had a high SD
area appearing as a voxel void which was seen on both the FOM and the ParaView plots
(fig3). The small high signal SD area was segmented and showed a 4x5mm area
with disorganised fibres on the FOM.
Subject 9’s SD map (fig4) showed an increase for voxels
corresponding to the origin of the ACL. The FOM showed fibres that were
identically orientated explaining the high SD values and was very clearly
visualised on the ParaView plots. A similar appearance was noted on subject 10’s
SD map (fig5).
During dissection subjects 9 and 10 were found
to have tearing and degeneration of the anteromedial band of the ACL. The
posterolateral band was intact in both cases. Subject 1 had an abnormal AC area
in the trochlear groove with no abnormality on the patellar AC surface. The
specialist orthopaedic veterinarian felt it was an area where the AC had been
replaced with fibrocartilage because it was spongy when probed. The area
measured 5mm x 4mm with a depth of around 1.5mm.Discussion
There was excellent correlation between the MADI and
dissection findings. Furthermore the
three subjects with notable high SD voxels on the SD map were also those found
to have pathology at dissection.
Subject 1’s possible fibrocartilage lesion needs further
exploration to explain how the MAE behaved in this instance. MAE was reduced in this region due to fibre
disorganisation. However the higher SD values of the voxels suggests a large
signal intensity variation that would only occur with a large MAE.
Subjects 9 and 10 partially torn anteromedial
bands of the ACL have an increased MAE at the ACL origin. We postulate that the highly uniform fibre
orientations resulted from the force that the ligament was under when it was
torn. The initial stretch of the fibres at the origin pulled the collagen fibres
into a highly aligned orientation as the bundle tore. The fibres have become
‘stuck’ in a highly aligned state rather than recoiling like elastic once the
tension was removed.Conclusion
The
spontaneous ligament rupture dog model has not been used in any previous magic
angle studies. The blinded spontaneous injury model found the MADI technique
could distinguish healthy and injured knees in a dog model providing proof of
concept. We chose this model as a natural injury would test the ability of the technique
to determine pathology with the associated physiological changes. Acknowledgements
We
are grateful to Charing Cross Hospital MRI department and Imaging Committee for
the kind use of the Siemens 3T Verio. We
would also like to thank the RVC PM technician Richard Prior for his assistance
with canine knee harvesting.References
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