Pamela Alejandra Franco1,2,3, Julio Sotelo1,2,3, Bram Ruijsink4,5, David Nordsletten4,5, Eric Kerfoot4,5, Joaquín Mura6, and Sergio Uribe1,3,7
1Biomedical Imaging Center, Pontificia Universidad Católica de Chile, Santiago, Chile, 2Electrical Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile, 3Millennium Nucleus for Cardiovascular Magnetic Resonance, Santiago, Chile, 4Guy's and St Thomas' NHS Foundation Trust, London, United Kingdom, 5School of Imaging Sciences and Biomedical Engineering, King's College London, London, United Kingdom, 6Department of Mechanical Engineering, Universidad Técnica Federico Santa María, Santiago, Chile, 7Radiology Department, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile
Synopsis
An important number of patients with dilated
cardiomyopathy have improved their left ventricular function with an optimal
treatment. However, it is not well understood whether remodeling represents a
recovery in left ventricular (LV) hemodinamics1. In this abstract,
we discuss the capacity of the ejection fraction to represent disease remission,
by analyzing LV blood flow.
Introduction
Left ventricular reverse remodeling (LVRR) has been
validated as a key prognostic tool in dilated cardiomyopathy (DCM)1.
This is defined as the improvement in LV function. Several studies have reported
an improvement above a threshold level, often set at LV ejection fraction
(LVEF) 50%1,2,3,4.
As ventricular flow is altered in the early stages of
remodeling, it is probable that the flow itself can influence disease
progression. Insights about the quantification of LV blood flow are available
now by 4D flow MR5. Previous studies have demonstrated altered LV
flow patterns in seemingly compensated DCM patients6,7. Therefore,
an LVEF-based definition of recovery may also be inadequate as subtle dysfunction
in cardiac strain or energetics, even in the presence of apparently normalized
LVEF8. The purpose of this study was to investigate the relationship
between LVEF and blood flow parameters during cardiac remodeling.Methods
Multi-slice 2D cine balanced steady-state
free-precession (b-SSFP) and 4D flow MRI data of 12 healthy volunteers and 13
DCM patients were acquired in a clinical MR Scanner of 1.5T (Philips Achieva, Best,
The Netherlands). At the time of diagnosis, DCM was defined as the presence of
symptoms and signs of heart failure with echocardiographic signs of ventricular
enlargement and systolic myocardial dysfunction in the absence of hypertension,
valve diseases or significant coronary artery disease sufficient to cause
global systolic impairment, in accordance with the definition of the European
Society of Cardiology9. Our DCM cohort all received treatment
following the heart failure guidelines. Five patients in the DCM cohort
responded to treatment, with an improved LVEF at the time of CMR imaging (range
51-66%).
To process the 4D Flor MRI, registration between the
multi-slice b-SSFP and the 4D Flow MRI data was used quaternion-derived
rotation matrix performed in the Eidolon Software (King’s College London,
London)10. Then, we double the number of slices in the b-SSFP
images. LV endocardium was delineated semi–automatically in
the short-axis b-SSFP images, using the software Segment v2.2 R6410 (Medviso
AB, Lund, Sweden)11-13. Segmentation curves were exported and
converted to LV binary mask and tetrahedral meshes were generated. We estimated
the cardiac phases under study, and we transfer the velocity information at
each node of the mesh from the 4D Flow MRI datasets using a cubic
interpolation. Then, using a finite element approach, we calculated velocity, energy
loss, vorticity, helicity density, viscous dissipation and kinetic energy14-15.
We used the software GraphPad Prism version 6.0.1 (GraphPad software Inc., San
Diego, California, USA) for all statistical analysis. Global hemodynamic parameters
were compared using the Kruskal-Wallis test, with statistical significance
assigned at p-values < 0.05. These data were displayed in box-whisker plots.Results
Table
1 shows the clinical data of DCM patients and volunteers. Statistical
differences were found in ejection fraction, LV end-diastolic volume, and LV
end- systolic volume. In figure 1, we show each global hemodynamic parameter vs
ejection fraction. It cannot be appreciated any linear relations between any
hemodynamic parameter and ejection fraction for the whole group of data. Figure
2 shows the 3D maps each hemodynamic parameter analyzed in this study, for one
representative healthy volunteer and two DCM patients: low and complete
responders to the cardiac resynchronization therapy. The DCM patients showed in
general smaller values than the healthy volunteers, in all the hemodynamic
parameters studied. Assessment of global parameters (Figure 3) showed
statistical difference between volunteers and DCM patients (low and complete
responders) at peak systole and e-wave in velocity magnitude (p < 0.0001*
p < 0.0001+, p = 0.0031* p = 0.0030+), vorticity
magnitude (p < 0.0001* p < 0.0001+, p = 0.0022*
p = 0.0025+), viscous dissipation (p < 0.0001* p <
0.0001+, p = 0.0024* p = 0.0024+), energy loss
(p < 0.0001* p < 0.0001+, p = 0.0028* p
= 0.0030+) and kinetic energy (p = 0.0025* p = 0.0025+,
p = 0.0030* p = 0.0029+). There were no statistical
differences in the parameters at end-diastole. Furthermore, we did not find
statistical differences between DCM groups. Conclusions
In DCM patients that show LVRR in response to
treatment, the flow derived hemodynamic parameters remained low. This shows
that LVEF is unable to reflect subtle ventricular dysfunction, which
potentially can be better assessed using flow-based parameters, because of
their sensitivity to abnormal pumping function5. Such analyses can
help to characterize the extent of LVRR and provide better prognostic
stratification1.Acknowledgements
This publication has
received funding from Millenium Science Initiative of the Ministry of Economy,
Development and Tourism, grant Nucleus for Cardiovascular Magnetic Resonance.
Also, has been supported by CONICYT
- PIA - Anillo ACT1416, CONICYT FONDEF/I Concurso IDeA en dos etapas
ID15|10284, FONDECYT #1181057. Sotelo J. thanks to FONDECYT Postdoctorado 2017
#3170737 and Franco P. thanks to CONICYT – PCHA/ Doctorado-Nacional/2018-21180391.References
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