Chunwei Ying1, Andria L. Ford2, Michael M. Binkley2, Yasheng Chen2, Peter Kang2, Jon Christensen1, Qing Wang1, Lisa Cash1, Jason Hassenstab2, Jin-Moo Lee1,2, Tammie L. S. Benzinger1,3, and Hongyu An1
1Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO, United States, 2Department of Neurology, Washington University School of Medicine, St. Louis, MO, United States, 3Department of Neurological Surgery, Washington University School of Medicine, St. Louis, MO, United States
Synopsis
Neuro-inflammation has been suggested as an important pathogenesis
factor for cerebral small vessel disease, but direct evidence in human
is lacking. In this study, we found that neuro-inflammation,
measured by 11C-PK11195 uptake, was associated
with white matter hyperintensities burden at baseline. More importantly, it predicted
cognitive decline in a longitudinal follow-up study.
Introduction
Fifty million people are affected by dementia
worldwide1. Vascular contributions to cognitive impairment and
dementia (VCID) is the 2nd leading cause of dementia after Alzheimer’s disease
(AD). White matter hyperintensities (WMH) is a major structural endpoint of cerebral
small vessel disease (CSVD), a major cause of VCID. The pathogenesis underlying
CSVD is not well understood. Several
systemic inflammatory markers were detected in blood and CSF2.
More recently, increased and persistent systemic inflammation (measured by CRP)
in midlife was found to promote late-life white matter structural injury3,
4. Activated microglia was found in post-mortem histological of CSVD
patients5. Therefore, neuro-inflammation has been suggested as an
important pathogenesis factor for CSVD. However, postmortem studies only reflect neuro-inflammation in the late
stages of disease and direct evidence of in vivo neuro-inflammation
as an early biomarker from humans is lacking. Expression of translocator protein (TSPO) is strongly upregulated upon microglial activation due to
neuro-inflammation. Therefore, PET tracers, such as 11C-PK11195,
that bind to TSPO allows imaging neuro-inflammation in vivo5.
In this study, we evaluated whether neuro-inflammation,
measured by 11C-PK11195 uptake, is associated with WMH burden at
time of imaging (baseline). Moreover, we aimed to examine whether neuro-inflammation
predicts cognitive decline in a longitudinal follow-up study. Due to a high prevalence of mixed CSVD and AD pathology in patients,
we also examined whether neuro-inflammation is associated with amyloid
deposition, a hallmark pathological feature of AD.Methods
Twenty-four elderly subjects (Age: 78 [64.75 83] (median
[IQR]); Education level: 16 [14 18] (median [IQR]) years; 14 female) were
recruited. 11C-PK11195 PET imaging were acquired from each subject. Two
11C-PiB PET scans were acquired. The 1st scan was
obtained 15.5 [12.5 20] (median [IQR]) months before the 11C-PK11195
PET from all subjects, and the 2nd scan was obtained 33 [27.75
80.75] months (median [IQR]) after the 11C-PK11195 PET from 18
subjects. MR FLAIR and T1 MPRAGE images were acquired 6 [2 15.5] (median [IQR])
months before 11C-PK11195 PET from 19 subjects. Cognitive data were collected using Knight
standard ADRC battery. The baseline cognitive data were collected 56.5 [-42.5
210.25] (median [IQR]) days after 11C-PK11195 PET and these patients
were followed up for 6.5 [4.25 11] (median [IQR]) years.
T1 MPRAGE images were segmented using Freesurfer software. 11C-PK11195
PET standardized uptake value (SUV) maps were computed. Standardized uptake value
ratio (SUVR) maps were computed with median cerebellum gray matter SUV as
reference. Mean cortical binding potential (MCBP) of 11C-PiB were
calculated using PET Unified pipeline (PUP) 6. WMH lesions were delineated
manually by a board-certified vascular neurologist on FLAIR images. To account
for brain volume variation across subjects, relative WMH volume (rVWMH) was
computed as a ratio of WMH lesion volume (VWMH) to brain tissue volume. All MR
and PET images and maps were aligned to International Consortium of Brain
Mapping (ICBM) brain atlas. Three standardized composite z-scores, zglobal,
zspeed and zmemory, were calculated to assess global, processing speed, and
memory cognitive functions respectively.Results
All patients had various levels of WMH lesion burden with a
median [IQR] VWMH of 8.3 [3.6 24.2] cm3, corresponding to rVWMH of
1.05% [0.35% 2.81%] (median [IQR]). These patients had relatively normal
cognitive functions at the time of imaging. Only 7 out of 24 subjects had a
baseline clinical dementia rate (CDR) greater than 0 (CDR=0.5: n=5, CDR=1: n=2).
A multivariate linear regression model
controlling for difference in acquisition date of 11C-PK11195 PET
and FLAIR showed that WMH lesion burden at baseline was significantly
associated with gray matter 11C-PK11195 uptake (p=0.011) and age (p=0.028).
However, 11C-PiB MCBP was not significantly associated with either
WMH lesion burden or 11C-PK11195 uptake, suggesting that neuro-inflammation
is independent of amyloid deposition.
Longitudinal analysis of cognitive
change over time using regional 11C-PK11195 SUVR and 11C-PiB
MCBP was performed using linear mixed-effects
regression with random subject effects and time delay between the acquisition
of PET data and baseline cognitive tests.
Interaction between PET tracer and age at cognitive evaluation was assessed as
primary outcome. We found that elevated gray matter 11C-PK11195
uptake and old age together predicted global cognitive decline (β=-0.85/year,
p=0.028) (Figure 1). We also found that elevated 11C-PiB MCBP of the
2nd 11C-PiB scan and old age predicted both global
cognitive decline (β=-0.1083/year, p<0.0001) and memory
impairment (β=-0.0819/year, p<0.0001), while the 11C-PiB
MCBP of the 1st 11C-PiB did not predict any cognitive decline.Discussion
11C-PK11195 uptake was associated with WMH lesion
burden at baseline. More importantly, elevated 11C-PK11195 and 11C-PiB
MCBP were not associated with each other and both predicted cognitive decline
in longitudinal follow-up, suggesting neuro-inflammation and amyloid deposition
may be independent pathogenesis factors for AD and/or CSVD.Conclusion
We found that 11C-PK11195 uptake was
significantly associated with baseline WMH lesion burden, but not with 11C-PiB
MCBP. A longitudinal follow-up study showed elevated 11C-PK11195
uptake in gray matter preceded cognitive impairment and neuro-inflammation and
old age together predicted cognitive decline. Our results suggest that
neuro-inflammation is an independent early biomarkers for neurodegenerative
disease.Acknowledgements
No acknowledgement found.References
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