Andrew T. Morgan1, Nils Nothnagel1, Jozien Goense1, and Lars Muckli1
1Institute of Neuroscience & Psychology, University of Glasgow, Glasgow, United Kingdom
Synopsis
Motivated by recent functional line-scanning
recordings in rodents, we developed a procedure to record human cortical layers
at high spatial (200 μm) and temporal resolution (100 ms). Our technique
addresses challenges associated with human line-scanning, such as planning
around cortical folding and restrictive SAR limitations. Our results show that
line-scanning of human cortical layers corroborates electrophysiological
measurements of tuning properties in primary visual cortex. These results
demonstrate that line-scanning is a promising technique for investigating local
functional circuits in human cortex.
Introduction
High-field functional MRI has pushed the spatial
resolution of fMRI to the sub-millimeter range, with 7T fMRI studies routinely
achieving 0.7-0.8 mm isotropic resolution. This increased resolution has
enabled the discrimination of mesoscopic signals from cortical columns1,2
and identification of laminar fMRI response properties within cortical areas3,4.
Despite these recent advances, it remains
challenging to compare human laminar fMRI signals to laminar profiles of electrophysiological
response properties5.
This is due to the size of typical fMRI voxels being larger than the thickness of
individual layers, thus leading to partial volume effects in which voxels
contain signals from multiple layers. This issue can be improved by acquiring highly
anisotropic data with increased resolution through cortical layers6.
However, the gyrencephalic nature of the human brain makes it difficult to line
up more than a small portion of the imaging plane with cortical lamination. This method is therefore limited to subjects with large sections of flat cortex.
Here, we aimed to close the gap between layer-dependent
fMRI and laminar electrophysiological recordings for human neuroscientific
studies. We developed a line-scanning procedure to record cortical layers at high
spatial resolution (200 μm), motivated by previous functional line-scanning recordings in rats7. Line-scanning
exploits anisotropic resolution (0.2x3x3 mm3
in this study) but limits the field-of-view to a single frequency
encoding line, allowing us to record high temporal resolution fMRI (100 ms) from any flat patch of cortex.
Our procedures produced recordings corroborating electrophysiological
measurements of tuning properties in primary visual cortex (V1). These results
serve as proof-of-principle that line scanning can be used to investigate local
functional circuits in human cortex.Methods
Planning: We scrutinized cortical folding
patterns in individual subjects prior to acquisition to minimize partial volume
effects. We generated a 3D cortical surface model using the Freesurfer package9
and marked vertices with the lowest 10% of Gaussian and mean curvature values
(Figure 1A). For our experiment, a flat cortical patch in V1 at least 3 mm wide
was chosen and the line-scanning frequency encoding direction was defined to
match the vertex normal of this patch.
Acquisition: Data were collected using a 7T
Siemens Magnetom Terra system (Siemens Healthcare, Erlangen, Germany) equipped
with a 1Tx/32Rx-channel head coil (Nova Medical Inc., MA, USA) and an SC72
gradient. Our line-scanning sequence was based on multi-echo gradient-spoiled
2D-FLASH (TR=100 ms, TEs=28, 41.5, and 55 ms, FOV=51x51x3 mm3,
matrix=256x256, resolution=0.2x0.2x3.0 mm3, flip angle=25º, readout BW=80 Hz/px). Saturation pulses were
applied every TR to suppress signal outside the line profile. A larger area was
saturated using a broad profile (BWTP=7, pulse duration=2 ms, thickness=50 mm,
flip angle=90º), followed by saturation of a thin area with a sharp profile near
the line (BWTP=45, pulse duration=7 ms, thickness=20 mm, flip angle=65º).
Three versions of the line-scanning sequence
were used. The first, a profile version, employed the standard
2D-FLASH phase encoding scheme (with and without saturation; Figure 1B). This
sequence was used to verify the location of the line. The second version was used for quantitative T2* and S0 mapping of the line.
The phase-encoding gradient was deactivated to achieve spatial encoding purely
along readout direction and we recorded 10 echoes (TR=1500 ms, TEs=15.3-54.9 ms
[4.4 ms spacing], readout BW=200 Hz/px). The third sequence again deactivated
the phase encoding gradient and was used for functional scanning (parameters
described above).
Functional experiment: The participant signed
consent as approved by the MVLS College, University of Glasgow. The participant
viewed two movie clips previously used in the Human Connectome Project (HO
‘Inception’ clip10
[2668 TRs] and ‘Vimeo repeat clip’ [1230 TRs]). Each clip was viewed three
times.
Analysis: Gray matter (GM) voxels were identified
via quantitative mapping (T2* and S0 fitting by exponential decay
curve). We identified Layer 4 via T2* values highlighting the Gennari
Line11. Functional
time courses from GM voxels were initially averaged to identify the population
receptive field (pRF) location of our cortical patch. We masked visual movie
features (orientation/spatial frequency) with 2D Gaussians to create time
course models12
(48 polar angle, 24 eccentricity locations) and the best-performing model was
chosen as the pRF-center (Polar Angle=1.36π, Eccentricity=3.84º visual angle). pRF location was then fixed, and pRF size and orientation tuning were computed
for each voxel using ridge regression. The sharpness of tuning was defined as
the difference in response magnitude between preferred and perpendicular
orientations.Results
The profile of our imaging
line is shown in Figure 2B. Within the line region, signal is attenuated by 43%
compared to the unsaturated scan. This loss is due to a combination of
imperfect saturation pulse profiles encroaching on the line, and loss of signal
due to a smaller excitation area. Outside the line region, we saturated 88.8%
of the signal.
Figure 3 shows that pRF
size is larger in deep and superficial layers, similar to previous results in both non-human primate electrophysiology13 and human fMRI14. Additionally, sharpness of tuning is higher in both
infragranular and supragranular layers compared to layer 4. This result
corroborates electrophysiological observations5,13 and extends beyond the current fMRI literature.Conclusion
V1 tuning properties computed from human
line-scanning support those in electrophysiological measurements. Line-scanning
is therefore a promising technique to investigate local functional circuits in
human cortex.Acknowledgements
This work was supported by the European Union’s
Horizon 2020 Framework Programme for Research and Innovation under the Specific
Grant Agreement No. 785907 (Human Brain Project SGA2), awarded to L.M., and by
the Medical Research Council (MR/R005745/1 awarded to J.G.; MR/N008537/1
awarded to L.M. and J.G.).References
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