Synopsis

Dissolution dynamic nuclear polarization (d-DNP) has made possible the in vivo measurements of biochemical reactions using MR spectroscopy and different imaging strategies. This presentation will focus on the available pre-clinical models used for d-DNP as well as a range of hyperpolarized ¹³C-labelled substrates that have been employed thus far. The talk will furthermore outline the necessary steps that have to be taken when planning pre-clinical d-DNP studies, ranging from the choice of model system over practical considerations to the actual hyperpolarized probes and their chemical modifications. The presentation will conclude by pointing towards potential future applications and optimizations of d-DNP.