The advance towards in vivo histology benefits greatly from the quantification of specific physical parameters1. The longitudinal relaxation rate (R1) has proven a reliable surrogate for myelination, facilitating investigation of the relationship between brain microstructure and function in vivo2–5. R1 maps can be estimated by combining spoiled gradient-echo volumes, acquired with variable flip angles, and calibration data correcting for flip angle inhomogeneities. The Bloch-Siegert shift (BSS) approach6 is a relatively time-efficient method that allows the calibration data to be acquired with an identical gradient-echo readout thereby matching distortions across all data needed to map R1. However, it requires data to be acquired at two off-resonance frequencies to remove B0 dependence up to second order and suffers from high specific-absorption-rate (SAR). Here, we investigate a modified BSS-based B1+ mapping approach that aimed to overcome these shortcomings by using a single offset frequency and a multi-echo readout.
The spatially-specific BSS, ΦBS caused by an RF pulse at off-resonance frequency ωRF in the presence of field inhomogeneity ωB0 is given by: $$\Phi_{BS}(x)=B_{1,peak}^2(x)\int_{0}^{T}\frac{(\gamma B_{1,normalized}(t))^2}{2(\omega_{B0}(x)-\omega_{RF})}dt = B_{1,peak}^2*K_{BS}(x) $$
Unlike the classic dual-offset BSS method6, here ΦBS is estimated from a single off-resonance frequency via a General Linear Model (GLM) applied to the phase data from a multi-echo readout (Fig1a). The design matrix includes regressors modelling (Fig1b):
1. the variation between even and odd echoes due to the bipolar readout;
2. the effect of the Bloch-Siegert pulse;
3. phase accrued with TE due to B0 inhomogeneities;
4. any common phase offset.
Given this formalism, the GLM also estimates ωB0 making the KBS term spatially-specific (whereas it is constant in the classic BSS approach). In this case, the B1+ estimation is theoretically completely independent of B0 inhomogeneity. Whereas the classic BSS method benefits from higher ωRF to minimise sensitivity to B0 inhomogeneity, this approach should be independent of B0. This multi-echo BSS (MEBSS) approach was tested in a gel phantom and in vivo using a single-channel transmit-receive head coil at 3T (Siemens Prisma). A 3D spoiled gradient-echo acquisition was used with an excitation flip angle of 12° and TR=60ms (50ms in phantom) with 4mm isotropic resolution. The BSS was induced by a 2ms-long Fermi pulse with 350° (320° in phantom) flip angle with four echoes (TE=2.30:2.30:9.20ms) acquired before (Fig1a) and five after (TE=14.98:2.30:24.18ms). In order to compare the MEBSS approach with the classic BSS approach, the data were acquired with interleaved positive and negative off-resonance frequencies with ωRF=±2, 4 and 8kHz. All echoes from a single offset frequency were used to calculate B1+ maps using the MEBSS approach whereas the first echo after the Bloch-Siegert pulse combining both off-resonance frequencies was used to estimate the B1+ map with the classic approach. A 3D median filter (kernel 5x5x5 voxels) was applied to all maps. Reference B1+ maps were acquired using a spin-echo/stimulated-echo method8,9 with a modified protocol for use with the single channel coil.
B1+ maps from the phantom (Fig.2a-d) illustrate the concordance of the different mapping methods. Quantitative histogram analysis indicates excellent agreement between the reference method and the classic BSS approach with ωRF= 8kHz, but greater variance and bias for the classic approach when ωRF is reduced to 2kHz. This can be expected due to increased sensitivity to B0 inhomogeneity. While the variance is larger for the proposed MEBSS approach, and bias still exists, the error remains less than 3% while only requiring half the scanning time.
In vivo, the spin-echo/stimulated-echo did not perform well because of artefacts due to the low bandwidth in the phase-encoded direction (due to a modified protocol without parallel imaging). Therefore, the dual-offset approach with ωRF=±8kHz was taken as reference. The proposed MEBSS B1+ maps were again of good quality. Although the relative error was greatest for this approach, it remained <5% (Fig.3). The error in the MEBSS B1+ maps did not correlate with B0 inhomogeneity (Fig.4a) or model residuals (Fig.4b). However, a theoretically unexpected dependence on ωRF was observed empirically (Fig.4c).
MFC is supported by the MRC and Spinal Research Charity through the ERA-NET Neuron joint call (MR/R000050/1). The Wellcome Centre for Human Neuroimaging is supported by core funding from the Wellcome [203147/Z/16/Z].
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