In preparation for a wider multi-site ‘travelling heads’ 7T fMRI study, we compare the performance of EPI distortion correction techniques for fMRI data across four sites, using three different 7T platforms. Specifically, we compare B0-map and phase-encoding reversal methods, applied to both task and resting state fMRI data.
Methods
This study was approved by local ethics review boards at each site and prior informed written consent was obtained from participants. Data were acquired in 3 healthy male participants (31±4 years) at four sites, using three different 7 Tesla whole-body MRI systems (one Philips Achieva, two Siemens Magnetom and one Siemens Terra scanners). The same model volume transmit, 32-channel receive head coil (Nova Medical) was used at each site and QA data on the day of scanning confirmed similar performance.
Both resting state (1s TR, multi-band 3) and motor-visual task (2s TR, multi-band 2) gradient echo (GE) EPI fMRI datasets4 were acquired (25ms TE, 1.5mm isotropic, echo spacing 0.68/0.75ms Siemens/Philips). For distortion correction, two separate reference scans were acquired (each 3 volumes), one with matched phase-encode direction, the other with reversed phase-encode direction. This was repeated for both GE and spin echo (SE) EPI reference scans (acquisition matrix, echo spacing, bandwidth matched to fMRI). Dual-echo GE B0-maps (1ms ΔTE) were also acquired at 2mm resolution. T2*-weighted anatomical (FLASH) data were acquired (0.75x0.75x1.5mm, TE 10ms, TR 1100ms) for image registration. All reference scans were acquired separately for, and immediately prior to, resting state and task fMRI datasets to match acquisition geometries.
With the exception of the different B0-map processing between Siemens and Philips (output data as phase difference and field map in Hz, respectively), a single analysis pipeline worked across all four sites. Phase-encoding reversal distortion correction was performed using FSL TOPUP,2 whilst the field-map method used FSL FUGUE.5 Quality of the distortion corrections were assessed through the residuals of rigid-body registration (correlation ratio cost function value) to the T2*-weighted anatomical and through assessing how the task-activation (FSL FEAT5) and resting state network (FSL MELODIC5) regions aligned to sulci in the T2*-weighted anatomical. Dice’s coefficient was used to measure relative spatial overlap between functional regions in image data generated using different distortion correction methods.
Results
The performance of SE phase-encoding reversal and fieldmap distortion correction methods was similar across all three platforms (Figure 1). Rigid body registration residuals (Figure 2) show that all distortion-corrected datasets have better alignment (lower residuals, p<0.001) to the T2*-weighted anatomical compared with the uncorrected data. This is also shown in the overlap of task-activation regions between the SE phase-encoding reversal and fieldmap methods, with better alignment to the anatomical sulci than in the uncorrected data (Figure 3). Whilst SE phase-encoding reversal and fieldmap methods performed similarly (Figure1/4A), there is a trend for the GE phase-encoding reversal method to perform less well (Figure 1/4B), as shown with a better alignment of default mode network regions to sulci in the T2*-weighted anatomical for SE phase-encoding reversal and fieldmap methods, compared with GE phase-encoding reversal and the uncorrected dataset. Note, the default mode network was chosen for its reproducibility across repeated experiments across sites.1. Shmuel A, Yacoub E, Chaimow D et al. Spatio-temporal point-spread function of fMRI signal in human gray matter at 7 Tesla. NeuroImage 2007;35:539-552.
2. Andersson J, Skare S and Ashburner B. How to correct susceptibility distortions in spin-echo echo-planar images: application to diffusion tensor imaging. NeuroImage. 2003;20:870-888.
3. Jezzard P and Balaban R. Correction for geometric distortion in echo planar images from B0 field variations. Magn. Reson. Med. 1995;34:65–73.
4. Moeller S, Yacoub E, Olman C et al. Multiband multislice GE-EPI at 7 tesla, with 16-fold acceleration using partial parallel imaging with application to high spatial and temporal whole-brain fMRI. Magn Reson Med. 2010;63:1144-1153.
5. Smith S, Jenkinson M, Woolrich, M et al. Advances in functional and structural MR image analysis and implementation as FSL. NeuroImage. 2004;23(S1):208-219.