Rakesh Kumar Gupta1, Indrajit Saha2, Anup Singh3, Pradeep Kumar Gupta1, Rupsa Bhattacharjee 2, Anandh K Ramaniharan 4, Rana Patir5, Sunita Ahlawat6, Jitender Saini7, and Marc Van Cauteren8
1Department of Radiology and Imaging, Fortis Memorial Research Institute, Gurgaon, India, 2Philips Health Systems, Philips India Limited, Gurgaon, India, 3Center for Biomedical Engineering, Indian Institute of Technology Delhi, Delhi, India, 4Philips Innovation Campus, Philips India Limited, Bangalore, India, 5Department of Neurosurgery, Fortis Memorial Research Institute, Gurgaon, India, 6SRL Diagnostics, Fortis Memorial Research Institute, Gurgaon, India, 7Department of Neuroimaging & Interventional Radiology, National Institute of Mental Health and Neurosciences, Bangalore, India, 8Philips HealthTech Asia Pacific, Tokyo, Japan
Synopsis
T1-perfusion MRI
derived relative cerebral blood volume (rCBV) is a key bio-marker for
pre-surgical grading of gliomas; however, acquiring clinically relevant higher
resolution T1-perfusion data with whole brain coverage is challenging due to
possible loss in temporal resolution. This study takes the advantage of combining
compressed-sensing with SENSE parallel-imaging i.e., Compressed-SENSE (CSENSE),
to develop high-resolution whole brain T1-perfusion with improved temporality. The
CSENSE enabled T1-perfusion derived rCBV values successfully differentiated
high and low grade gliomas and matched with the histopathological grading. The rCBV
cut-off value from CSENSE assisted T1-perfusion was similar to the routine T1-perfusion without-CSENSE
of histopathology-matched gliomas.
Introduction:
T1-perfusion MRI is
one of the noninvasive quantitative methods to estimate hemodynamic and kinetic
parameters for diagnosis and management of human gliomas [1]. To develop a
robust whole-brain T1-perfusion method for generating quantitative data with
higher spatial and temporal resolution by overcoming current clinical
limitations, methods like compressed sensing [2] for MR perfusion imaging has
already been realized [3,4]. In this study, compressed sensing technology is
combined with the SENSE parallel-imaging
infrastructure, i.e., Compressed-SENSE (CSENSE), for high-resolution dynamic T1-perfusion data
acquisition in human brain by exploiting the multi-element receiver coil
sensitivity variation and sparsity constraining. This study also examines the
efficacy of this quantitative-CSENSE method in deriving key quantitative parameter
of T1-perfusion to differentiate tumor grades, i.e., relative cerebral blood
volume (rCBV), by comparing the CSENSE accelerated perfusion results with
histopathological grading of gliomas along with the rCBV cut-off value of histopathology-matched
tumors previously acquired without CSENSE acceleration.Methods:
In this study, CSENSE enabled
brain T1-perfusion data acquisition at 3.0 T (Ingenia, Philips, The
Netherlands) included treatment naïve 26 patients; 19 with high grade and 7
with low grade gliomas.
To quantify voxel-wise pre-contrast tissue
longitudinal relaxation time T10 for computing leakage corrected CBV
values [1], pre-contrast 2D T1-weighted TSE (TR/TE 360/10 ms), fast dual SE
proton-density and T2-weighted fat
suppressed images (TR/TE1/TE2 3500/23.2/90 ms) were acquired with 20 slices of
thickness 6 mm, FOV= 230 × 230 mm2, reconstructed matrix= 256 x 256 and
SENSE factor of 2. T1 dynamic perfusion images were acquired using a 3D T1-FFE
with CSENSE factor 3.75. At the fourth time point of the dynamic data
acquisition, 0.1 mmol/kg body weight of Gd-BOPTA (Multihance, Bracco, Italy)
was administered intravenously at a rate of 3.0 ml/sec, followed by a 30-ml
saline flush. A series of 640 images at 32 dynamics from 20 slices were
acquired with a temporal resolution of 3.8 sec. T1 perfusion maps were
generated using a home written script based on Leaky Tracer Kinetic Model of
T1-perfusion [5,6]. Two experienced radiologists, blinded to the final
histopathology, performed the ROI analysis by placing it on the slice showing
the tumor with maximum rCBV and recorded the values.
The rCBV values obtained from CSENSE-assisted
T1-perfusion scan were compared with 26 histopathology-matched T1-perfusion
data previously acquired without CSENSE and with 12 slices, FOV = 240X240 mm2
and matrix size = 128X128 with a temporal resolution of 3.95 s.
All statistical analyses were performed with SPSS
16 software. Receiver operating characteristic (ROC) curve analysis was used to
evaluate the performance of rCBV with and without CSENSE acceleration in
discriminating between the high and low grades of tumors by comparing the area
under the curve (AUC). A p value of <0.05 indicated a statistically
significant difference.Results:
Compressed-SENSE
accelerated T1-perfusion generated rCBV (Figure 1) values were able to successfully
differentiate high grade gliomas from low grades with high sensitivity and
specificity. The cut off between high grade and low-grade gliomas for high spatial
resolution whole brain CSENSE enabled T1-perfusion is 2.42 and matched the non-CSENSE
accelerated 12 slice data (Table-1, figure 1). Discussion:
T1-perfusion has the advantage over commonly used
T2* based dynamic susceptibility contrast (DSC) perfusion as it provides both
the quantitative hemodynamic and kinetic parameters of tumor, and it reliably generates
rCBV values for the tumors in the presence of blood, calcifications, proximity
to skull-base and in post-surgical lesions [7,8].
It is well known that perfusion derived cerebral blood volume correlates
with tumor neoangiogenesis and hence the rCBV obtained from T1-perfusion MRI is
considered one of the robust noninvasive methods for pre-operative tumor
grading. However, acquiring higher resolution T1-perfusion data with whole
brain coverage is challenging and may increase acquisition time at the cost of
temporal resolution that is essential for quantification of CBV. This study
takes the advantage of CSENSE acceleration to develop an imaging methodology so
that higher resolution T1-perfusion data in terms of both spatial and temporal
resolution with whole brain coverage can be acquired with higher temporal
resolution. The rCBV obtained from CSENSE accelerated T1-perfusion data was
accurate as it matched with histopathology grading to differentiate high and
low grade gliomas in this study. Moreover, the rCBV cut-off to separate high
grade from low grade gliomas was comparable with the cut-off obtained from
histology-matched non-CSENSE accelerated T1-perfusion data. Conclusion:
This study demonstrates the possibility to obtain higher spatial and temporal resolution quantitative T1-perfusion data with whole brain coverage using CSENSE accelerated T1 dynamic scans; and the rCBV generated using this method may be used for pre-surgical evaluation of glioma. Acknowledgements
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