Lei Li1, Xueling Suo1, and Qiyong Gong1
1Department of Radiology, Huaxi MR Research Center, Chengdu, China
Synopsis
Paroxysmal
kinesigenic dyskinesia (PKD) is a rare movement disorder characterized by
sudden, brief attacks of involuntary movements. This study aims to detect the
topological organization of white matter structural connectivity in PKD with and
without PRRT2 mutations using graph theoretical approaches. Compared with
non-PRRT2 mutation carriers, PRRT2 mutations carriers are significantly
associated with a younger age of onset, a complicated form of PKD, combined
phenotypes of dystonia and chorea, and a tendency for a family history of PKD
in our population, as well as showed topological trends for randomization.
Introduction
Paroxysmal kinesigenic dyskinesia (PKD) is a rare
movement disorder characterized by sudden, brief attacks of involuntary
movements that are precipitated by sudden voluntary movement. Though
there are no apparent morphological changes, the dysfunction of the basal ganglia-thalamocortical pathway
are proposed as the neuroanatomical
basis of the disease1. Recently PRRT2 (proline-rich
transmembrane protein 2) gene has been identified as a
causative gene of PKD , there are still a quite proportion of PKD patients identified without the mutation of PRRT2,
and the pathogenic mechanism of the mutation remains
largely unknown. PKD patients with PRRT2 mutations are usually associated with an earlier age at onset, longer
duration of attacks, a complicated form of PKD, combined phenotypes of dystonia
and chorea, and a tendency for a family history of PKD2, however, the effect of PRRT2 mutation on anatomical networks in PKD
patients remains largely unknown. This study aims to detect the topological
organization of white matter structural connectivity in PKD with and
without PRRT2 mutations using graph theoretical approaches.Methods
We recruited 20 idiopathic PKD patients with PRRT2
mutations, and 26 without PRRT2 mutations. Detailed clinical
information on demographics, nature of attacks, family history and the presence
of associated conditions were obtained by in-person interviews using a
standardized questionnaire. We extracted DNA from
blood samples of the study subjects. MR imaging data were acquired with a 3.0-T MR imaging system by using
a single-shot spin-echo echoplanar image
sequence that included one high-resolution T1
image and one DT imaging data image. All the image
preprocessing and analyses were implemented by using
PANDA (a pipeline tool for diffusion MR imaging). The structural connectome was constructed by using DT
imaging tractography and thresholding the mean fractional anisotropy of 90 brain
regions to yield 90 × 90 partial correlation matrixes. Graph theory analysis
was used to examine the group-specific topologic properties by using the GRETNA toolbox investigating the
topologic properties of brain networks at both the global and nodal level,
and nonparametric permutation tests were used for group comparisons of
topologic metrics.Results
Compared with non-PRRT2 mutation carriers (mean age at initial onset was 12.5 ± 2.8 years), the PRRT2 mutation carriers were younger
at onset (mean age at initial onset was 10.7 ± 5.6 years); and present with complicated PKD, combined phenotypes of
dystonia and chorea. Moreover, 15 among 20 PRRT2 mutation carriers reported a positive identified or suspected family history, while only one non-PRRT2 mutation carrier reported his mother has a family history of seizures. Both
groups exhibited small-world topology. However, PRRT2 mutation carriers showed topological
trends for randomization, demonstrated as lower normalized characteristic path length
λ (P = 0.017) compared
with controls. Furthermore, the patients demonstrated significantly decreased
nodal centralities in the left paracentral lobule, left inferior parietal lobule,
left angular gyrus, right Heschl’s gyrus and left middle and post cingulum. The
PKD-related alterations in anatomic connections involving in
the paracentral- inferior pariental- middle cingulum
circuit.Discussion
Consistent with previous studies, PRRT2
mutations carriers are significantly associated with a younger age
of onset, a complicated form of PKD, combined phenotypes of
dystonia and chorea, and a tendency for a family history of PKD in our
population. Meanwhile, the PRRT2 mutation carriers showed significant decrease
in the normalized characteristic path length, suggesting a shift
toward random network. In addition to the global topologies, one of the decreased
nodal activities we observed was the left paracentral lobule, which is very
important in the movement disorder since it controls motor and sensory innervations of
the contralateral lower extremity. As dystonia is commonly viewed as a sequence of motor dysfunction, our results provided an anatomic basis
for the pathophysiological mechanisms of PKD.Conclusion
In conclusion, from the clinico-genetic-anatomic
aspects, our studies comparing PKD patients with and without the mutation of PRRT2 provide topologic insights into understanding the pathophysiological
mechanisms of PKD. However, direct correlations and interactions
of the clinico-genetic-anatomic factors should be investigated in future.Acknowledgements
No acknowledgement found.References
1,
Long Z et al., Thalamocortical dysconnectivity in paroxysmal kinesigenic dyskinesia:
Combining functional magnetic resonance imaging and diffusion tensor imaging. Mov
Disord. 2017 Apr;32(4):592-600
2, Huang XJ et al., Paroxysmal
kinesigenic dyskinesia: Clinical and genetic analyses of 110 patients. Neurology. 2015 Nov 3;85(18):1546-53