Adam Clemente1, Emma Lawrence1, Phoebe Imms1, Derek K Jones1,2, and Karen Caeyenberghs1
1School of Psychology, Australian Catholic University, Melbourne, Australia, 2School of Psychology, Cardiff University Brain Research Imaging Centre, Cardiff, United Kingdom
Synopsis
There is evidence
showing the neural basis of cognitive control utilizing morphological measures
of the brain in healthy adults. In the present study, we complement and extend
on previous voxel-based morphometry based research by utilizing the more
specific brain macrostructure metric of cortical thickness to investigate the differential
morphological correlates of objective and subjective cognitive control. Here,
we used a rigorous cognitive control test battery implemented on 25 healthy
adults. Further research on morphological correlates of objective and
subjective cognitive control in healthy populations is necessary to provide
baseline data for future clinical populations.
Introduction
Several studies have
investigated the neural basis of cognitive control utilizing morphological
measures in healthy adults1-4. However, the majority of these
studies have utilized the voxel-based morphometry (VBM) approach employing measures
such as grey matter density4-6 that are less sensitive than quantitative metrics (e.g., Freesurfer)7. In addition, most studies have focused on
“objective” cognitive control, which is an individual’s cognitive ability for a
specific cognitive function8. Most studies have neglected
“subjective” cognitive control, or one’s self-reporting of their ability to
perform cognitive tasks, which captures a different process to “objective”
cognitive control8. To overcome these drawbacks, the current study
will employ cortical thickness as a specific metric of brain macrostructure to
investigate the morphological correlates of objective and subjective cognitive
control in healthy adults.Materials and Methods
Participants: 25 healthy
adults (Mage=27.68 years; SD=9.36) completed a cognitive test
battery including both objective and subjective measures of cognitive control.
Objective cognitive control was measured via three subtests of the computerized
Psychological Experimental Building Language (PEBL)9 test battery including corsi-blocks (visuospatial memory), digit span (verbal working memory), and
vigilance (sustained attention). Subjective cognitive control was self-reported
utilizing four subscales of two questionnaires, including the Memory/Attention
subscale of the Neurobehavioural Functional Inventory (NFI)10 and
the Verbal Memory, Visuospatial Memory, and Attention subscales of the Multiple
Ability Self-Report Questionnaire (MASQ)11.
MRI: Anatomical scans were acquired on a
Siemens 3T Skyra MRI scanner (Siemens, Erlangen, Germany) with a
32-channel head coil using a three-dimensional magnetisation-prepared rapid gradient-echo (3D-MP RAGE) sequence with the following parameters: TR/TE=2530ms/2.58ms;
flip angle=7deg; FOV=220x220mm2; resolution=0.9x0.9x0.9mm3;
number of slices=176; TA=6min.
Preprocessing: Freesurfer
software version 5.312 was used for cortical
reconstruction and volumetric segmentation of the brain surface using a
semi-automated approach. An example of the Freesurfer pipeline can be seen in Figure
1. Six regions from the Desikan-Killany atlas13 were
selected to define regions of interest (ROIs) comprising the cognitive control
network14. Cortical thickness values were extracted for each ROI for
left and right hemispheres.
The results for each subject were
carefully inspected to ensure the accuracy of the skull stripping, segmentation,
and cortical surface reconstruction.
Preliminary Correlation Analyses
Cortical thickness and objective cognition: There
was a significant positive correlation
between mean reaction time (RT) in the objective sustained attention test and
cortical thickness of the rostral part of the right middle frontal gyrus (r=.51, p=.012, Figure 2). This indicates that increased cortical thickness is
associated with worse sustained attention performance. Furthermore, a moderate
positive correlation was found between objective visuospatial memory span and
cortical thickness of the pars opercularis of the left inferior frontal gyrus (r=.42, p=.035, Figure 2). This relationship indicates that increased cortical
thickness is associated with greater visuospatial memory abilities. There were
no significant relationships found between participant’s cortical thickness and
objective verbal working memory (all p’s>.10).
Cortical thickness and subjective cognition: A significant moderate negative relationship was
found between the subjective NFI Memory/Attention subscale and cortical
thickness of the pars triangularis of the right inferior frontal gyrus (r=-.41, p= .041, Figure 2). Hence, increased cortical
thickness was associated with lower subjective reporting of memory and
attention impairments.
For the MASQ questionnaire, results revealed a
significant moderate negative relationship between visuospatial memory subscale
and the rostral part of the right middle frontal gyrus (r= -.58, p= .005, Figure 2, survived FDR correction).
In other words, increased cortical thickness was associated with lower
subjective self-reported visuospatial memory impairments. Important to note, no
other significant correlations were observed between cortical thickness and
both Verbal Memory and Attention subscales (all p’s>.10).
Objective and subjective cognition: Results
revealed no significant correlations between participant’s objective and
subjective cognitive control. This was across the objective measures three subtests and all subscales of both subjective measures (all p’s>.10). Scatterplots can be seen in Figure 3.
Discussion and Conclusions
Our work
complements and extends previous studies that used qualitative VBM-metrics, and
used quantitative measures of cortical thickness. It is the first to investigate and provide
preliminary evidence for differential morphological correlates of objective and
subjective cognitive control in healthy adults. The lack of correlations
between objective and subjective cognitive control measures may be due to (a)
the study not being sufficiently powered or; (b) the range of subjective cognition being insufficiently large in the
healthy population to reveal a correlation. Data acquisition of healthy adults is
ongoing due to potential power problems. In conclusion, this study provides
important preliminary insights into the differential morphological correlates
of subjective and objective cognition, which will serve as a platform for
targeted investigations in clinical populations (e.g., Traumatic Brain Injury patients) where these aspects of
cognition are differentially impaired.Acknowledgements
This
work was facilitated by an ACURF Program grant awarded to Karen Caeyenberghs by
the Australian Catholic University (ACU). The authors have no conflict of
interest to declare.References
- Antonova E, Kumari V, Morris R, et al.
The relationship of structural alterations to cognitive deficits in
schizophrenia: A voxel-based morphometry study. Biol Psychol. 2005;58(6):457-467.
- Salthouse TA, Habeck C, Razlighi Q, et al.
Breadth and age-dependency of relations between cortical thickness and
cognition. Neurobiol Aging. 2015;36(11):3020-3028.
- Schmidt-Wilcke T, Luerding R, Weigand T, et
al. Striatal grey matter increase in patients suffering from fibromyalgia–a
voxel-based morphometry study. Pain. 2007;132:S109-S116.
- Gautam P, Anstey KJ, Wen W, et al.
Cortical gyrification and its relationships with cortical volume, cortical
thickness, and cognitive performance in healthy mid-life adults. Behav Brain Res. 2015;287:331-339.
- Bookstein FL. “Voxel-based morphometry”
should not be used with imperfectly registered images. Neuroimage. 2001;14(6):1454-1462.
- Crum WR, Griffin LD, Hill DL, et al. Zen
and the art of medical image registration: correspondence, homology, and quality.
NeuroImage. 2003;20(3):1425-1437.
- Eriksson SH, Free SL,
Thom M, et al. Quantitative grey matter histological measures do not correlate
with grey matter probability values from in vivo MRI in the temporal lobe. J
Neurosci Methods. 2009; 181(1): 111-118
- Miskowiak KW, Petersen JZ, Ott CV, et al.
Predictors of the discrepancy between objective and subjective cognition in
bipolar disorder: A novel methodology. Acta
Psychiat Scand. 2016;134(6):511-521.
- Mueller ST, Piper BJ. The psychology
experiment building language (PEBL) and PEBL test battery. J Neurosci Meth. 2014;222:250-259.
- Kreutzer JS, Marwitz JH, Seel R, et al.
Validation of a neurobehavioral functioning inventory for adults with traumatic
brain injury. Arch Phys Med Rehab.
1996;77(2):116-124.
- Seidenberg M, Haltiner A, Taylor MA, et
al. Development and validation of a multiple ability self-report questionnaire.
J Clin Exp Neuropsyc. 1994;16(1):93-104.
- Fischl B, Dale AM. Measuring the
thickness of the human cerebral cortex from magnetic resonance images. P Natl Acad Sc USA. 2000;97(20):11050-11055.
- Desikan RS, Ségonne F, Fischl B, et al.
An automated labeling system for subdividing the human cerebral cortex on MRI
scans into gyral based regions of interest. Neuroimage. 2006;31(3):968-980.
- Metzler-Baddeley C, Caeyenberghs K, Foley
S, et al. Task complexity and location specific changes of cortical thickness
in executive and salience networks after working memory training. NeuroImage.
2016;130:48-62.