The hemodynamics of the liver in normal and diseased conditions are not fully understood. In this study, 4D flow MRI and computational modeling were used to analyze the effects of portal venous flow patterns at the spleno-mesenteric confluence. Specifically, the geometric configuration of the confluence on intra-hepatic portal circulation in healthy subjects and cirrhotic patients before and after a meal challenge was analyzed. Significant correlations between flow distribution, helicity, geometry, and flow patterns were observed, and differences between normal and pathological flow were also characterized.
Liver disease can have heterogeneous lobar distribution, which may be a result of imbalanced blood flow distribution between portal venous branches. The hepatic blood flow magnitude and distribution can be characterized to provide insight into the pathophysiology of liver disease that affects or depends on the hepatic vasculature.[1],[2] However, the underlying patterns and dynamics of hepatic blood flow have not yet been comprehensively studied.
One particular pattern that has been observed in the hepatic portal vein (PV) is a helical flow structure that develops distal to the confluence of the splenic vein (SV) and superior mesenteric vein (SMV).[3] The cause and downstream effects of this portal helix flow pattern on the surrounding vasculature in states of health and disease are currently not well understood. Emerging imaging and computational methods to visualize and quantify blood flow, however, now make the evaluation of flow patterns at the spleno-mesenteric confluence (SMC) feasible.[4],[5],[6]
Thus, the purpose of this study was to use 4D flow MRI and computational simulation to examine the effects of the portal helix flow pattern and SMC geometry on intra-hepatic portal hemodynamics.
In this IRB-approved and HIPAA-compliant study, 12 subjects (6 healthy, 6 cirrhotic) were imaged before and after a meal challenge[2] on a clinical 3T MRI system (Discovery MR 750, GE Healthcare, Waukesha, WI) with a 32-chanel body coil (NeoCoil, Pewaukee, WI). 4D velocity mapping was performed using a cardiac-gated time-resolved 3D radially undersampled phase contrast (PC) acquisition (5-point PC-VIPR) with increased velocity sensitivity performance. [7] Acquisition and reconstruction parameters were previously described.[2],[6]
3D models, segmented from PC angiograms using MIMICS (Materialise, Leuven, Belgium), were exported to 3-matic (Materialise, Leuven, Belgium) to measure the angle of the SMC confluence (Figure 1).
Segmented models, along with the 4D flow data were exported to Ensight (CEI, Apex, NC). Cut-planes were placed in the splenic vein (SV), superior mesenteric vein (SMV), and the left (LPV) and right (RPV) portal veins, where flow measurements were made. Velocity streamlines were generated from the SV and SMV planes, and tracked throughout the portal vein (Figure 2). The number of streamlines exiting the LPV and RPV were quantified to represent right and left liver lobe flows. Helicity, vorticity, and kinetic energy were also quantified throughout the portal anatomy.
In some cirrhotic patient cases, limited SNR performance inhibited reliable quantification and visualization of flow distribution in distal portions of the portal vein. Therefore, MRI based computational simulation [6] was used to augment distal portal vein flow distribution analysis in these cases. 4D flow MRI data were then compared across pre/post meal challenge and healthy/cirrhotic subject categories using a Student’s paired t-test and linear regression for correlation analysis.
[1] Lara, M., Chen, C. Y., Mannor, P., Dur, O., Menon, P. G., Yoganathan, A. P., and Pekkan, K., 2011, "Hemodynamics of the hepatic venous three-vessel confluences using particle image velocimetry," Ann Biomed Eng, 39(9), pp. 2398-2416.
[2] Roldán-Alzate, A., Frydrychowicz, A., Said, A., Johnson, K. M., Francois, C. J., Wieben, O., and Reeder, S. B., 2015, "Impaired regulation of portal venous flow in response to a meal challenge as quantified by 4D flow MRI," J Magn Reson Imaging, 42(4), pp. 1009-1017.
[3] Van As, A. B., Hickman, R., Engelbrecht, G. H., Makan, P., Duminy, F., and Kahn, D., 2001, "Significance of the portal vein helix," S Afr J Surg, 39(2), pp. 50-52.
[4] Roldán-Alzate, A., Frydrychowicz, A., Niespodzany, E., Landgraf, B. R., Johnson, K. M., Wieben, O., and Reeder, S. B., 2013, "In vivo validation of 4D flow MRI for assessing the hemodynamics of portal hypertension," J Magn Reson Imaging, 37(5), pp. 1100-1108.
[5] Rosenthal, S. J., Harrison, L. A., Baxter, K. G., Wetzel, L. H., Cox, G. G., and Batnitzky, S., 1995, "Doppler US of helical flow in the portal vein," Radiographics, 15(5), pp. 1103-1111.
[6] Rutkowski, D. R., Reeder, S. B., Fernandez, L. A., and Roldán-Alzate, A., 2017, "Surgical planning for living donor liver transplant using 4D flow MRI, computational fluid dynamics and in vitro experiments," Computer Methods in Biomechanics and Biomedical Engineering: Imaging & Visualization, pp. 1-11.
[7] Johnson, K. M., Lum, D. P., Turski, P. A., Block, W. F., Mistretta, C. A., and Wieben, O., 2008, "Improved 3D phase contrast MRI with off-resonance corrected dual echo VIPR," Magn Reson Med, 60(6), pp. 1329-1336.