Reduced plantar sensation can lead to weakened balance ability in elder adults and an addition of cognitive tasks will further weaken it. Thereby, we attempted to explore the brain activity pattern of the elder and of young adults under foot stimuli and in dual-task condition. The results revealed that elder adults have significantly stronger cortical excitability than young do, and that foot stimuli induced stronger cortical excitability compared with dual-task condition. In conclusion, these phenomena may be due to the elder adults’ inadequate central reserve. Besides, added cognitive task can further reduce the brain’s response through diminished sensory input.
Subjects:
16 healthy young adults (22.2 ± 2.1 years; 11 males) and sixteen healthy elder adults (61.8 ± 6.5 years; 9 males) were recruited for this study. All subjects provided informed consent as approved by the Institutional Review Board of Peking University First Hospital, Beijing.
Protocol:
In the fMRI protocol, each block was 30s in duration and repeated three times(Fig.1). The first part of the fMRI protocol was used to compare the brain response patterns of elder and young adults under the foot stimuli and the second was used to further understand the response of dual-task in elder adults.
MRI images acquisition
MRI scans were performed on a GE 3T scanner (Signa Excite HD). BOLD data were acquired using a standard echo-planar imaging sequence (repetition time/echo time, 2000/30 ms; flip angle, 90°; image matrix, 64×64; thickness/spacing, 4 mm/1 mm; field of view, 230×230 mm2; 28 interleaved axial slices; and 30 repetition times.) A high-resolution structural image was acquired using a three-dimensional fast spoiled gradient echo sequence for anatomical localization (repetition time/echo time, 7.8/3.0 ms; flip angles, 20°; inversion time, 450 ms; field of view, 240×240 mm2; slice thickness, 2.0 mm with 1.0 mm overlap; in-plane resolution, 1×1 mm2.)
Data and statistical analysis
BOLD data were preprocessed with Statistical Parametric Mapping software (SPM8). Next, one sample t-test was used to obtain group analysis results. In addition, two sample t-tests were used to explore difference of brain activity patterns between conditions. Brain regions related to sensory processing of the foot and lower limbs are defined as regions of interest (ROIs), which include the bilateral dorsomedial somatosensory cortex (dmS1) (Fig.2). And the average percent signal change (PSC) value of each ROI was calculated. Then, the differences of PSC values within each ROI were analyzed through two-tailed paired t-tests.
Two sample t-test results showed that compared to young adults, foot stimuli to elder adults elicits increased excitability of bilateral postcentral gyrus, precentral gyrus, supplementary motor area, middle frontal gyrus, inferior parietal lobule, superior parietal lobule, middle cingulate gyrus, angular gyrus, supramarginal gyrus, superior temporal gyrus, middle temporal gyrus, left precuneus, right superior frontal gyrus, and insula (uncorrected, p < 0.001, cluster size ≥ 10).
Whole-brain group-level comparison revealed that, in comparison dual-task condition foot stimuli only was associated with greater activation within the bilateral supplementary motor area, superior frontal gyrus, left side of central lobule, angular gyrus, middle frontal gyrus, right postcentral gyrus, superior parietal gyrus, and supramarginal gyrus in elder adults (uncorrected, p < 0.001, cluster size ≥ 10).
ROI analysis further showed that there was no significant difference in the excitability of the left dmS1 and the right dmS1 between young and elder adults (Fig.4). ROI analysis also revealed that, as compared to foot stimuli only condition, the left dmS1 (p < 0.05) as well as the right dmS1 (p < 0.05) were significantly inhibited under the dual-task condition in elder adults (Fig.4.).
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