Isabell Katrin Bones1, Anita A Harteveld1, Suzanne L Franklin1,2, Matthias JP van Osch2, Jeroen Hendrikse3, Chrit TW Moonen1, Clemens Bos1, and Marijn van Stralen1
1Center for Image Sciences, University Medical Center Utrecht, Utrecht, Netherlands, 2C.J.Gorter Center for High Field MRI, Leiden University Medical Center, Leiden, Netherlands, 3Radiology, University Medical Center Utrecht, Utrecht, Netherlands
Synopsis
Aiming for rapid and
accurate perfusion measurement, background suppressed (BGS) ASL under free
breathing is desired. Motion compensation on BGS ASL is
challenging due to the lack of anatomical contrast. We
investigated the benefit of BGS versus non-BGS ASL, guided by motion compensation
based on the ASL-images themselves and additionally acquired fat-images.
Registration effect on perfusion weighted signal (PWS) and temporal SNR (tSNR) was
evaluated for ASL-image and fat-image based registration, proving increased tSNR and increased PWS robustness, without compromising
signal intensity. We conclude that free-breathing BGS renal pCASL with image-based retrospective motion compensation
yields better reproducibility than without BGS.
Introduction
Perfusion imaging using subtraction-based
arterial spin labeling (ASL) MRI is inherently limited by low SNR and is
affected by physiological, image noise and abdominal bulk motion. To overcome
these limitations for renal ASL, (1) background suppression (BGS) has been shown to increase
sensitivity1 and (2) motion compensation strategies, such as breath-holding,
synchronized breathing and image-based registration have been shown to reduce subtraction
artifacts2. Aiming for rapid and accurate perfusion measurement, BGS
ASL under free breathing (FB) is desired, alleviating patient cooperation for
abdominal ASL. However, image-based registration of ASL images might be affected
by the lack of anatomical contrast in BGS ASL and therefore the use of surrogate
motion signals has been proposed2.
Inspired by recent work that employed fat-images
for motion compensation of DCE-MRI3,4, we investigated its
feasibility for BGS ASL. To this end, we compared BGS versus
non-BGS ASL, guided by motion compensation based on the ASL-images themselves
and the additional fat-images.Methods
Imaging:
Seven volunteers (age 24 - 43,
3 men) were scanned on a 1.5T-MRI (Achieva, Philips, NL) using a 16-element
torso coil. Pseudo-continuous ASL (pCASL) was acquired with a gradient echo EPI
readout (see Table 1), with background suppression (BGS) and without (non-BGS),
in single-slice (SS) and multi-slice (MS; 3slices) acquisitions. BGS using two
inversion pulses was optimized to have slightly positive static tissue signal,
avoiding sign changes in label images that would corrupt perfusion signal.
Within the same sequence, fat-images were acquired as a second phase readout
with a phase interval of 200 ms and 324 ms for SS and MS, respectively (Figure
1), allowing the fat signal to recover from saturation of the SPIR fat
suppression pulse preceding the regular ASL image acquisition.
Analysis:
Prior to registration, fat-images
were corrected for the water-fat shift in feet/head direction. Kidneys where
manually segmented in the first ASL dynamic. Segmentations served
kidney-specific motion compensation and ROI analysis. Motion compensation was
performed, for each kidney separately, by rigid registration (3D MS vs 2D SS)
of all ASL-images to the first dynamic directly (ASLReg), or via registration
of their corresponding fat-images (FatReg). Absence of motion between the ASL
and fat-image was assumed. The control image of the non-BGS acquisition served
as M0 estimate, and was co-registered via the fat-image to the non-BGS and BGS
ASL.
After registration, subtraction images were averaged over all dynamics
and expressed as relative signal difference to M0, yielding perfusion weighted
images (PWIs). Voxel-wise temporal SNR (tSNR) within the ROI of the PWIs was
expressed as the ratio of the mean PWI voxel-wise perfusion weighted signal over
time and the temporal voxel-wise standard deviation of PWI. tSNR was evaluated
as a surrogate for the precision of the perfusion measurement.
Results
Fat-image guided retrospective motion compensation was accomplished for all
seven volunteers, despite varying fat contour quantity (Figure 2). FatReg
showed a relative tSNR increase of 17% for MS and SS, respectively, compared
with no registration (NoReg), for both MS and SS. The higher tSNR increase for
SS series demonstrates that the recovery delay induced kidney position mismatch
influences FatReg performance for perfusion weighted signal (PWS). Retrospective image registration
significantly improved tSNR using the conventional ASLReg (Wilcoxon, p<0.05)
and the proposed FatReg (Wilcoxon, p<0.05) technique compared with NoReg
(Table 2). This is consistently reflected in a higher robustness of the PWS, as
implied by an improved agreement of ASLReg and FatReg, with and without BGS
(Figure 3). Applying BGS resulted in an average tSNR increase of 70% and did
not reduce the PWS for either registration technique. Surprisingly, for both non-BGS
and BGS, ASLReg yielded better tSNR than FatReg for image registration (Table 2). Discussion and conclusions
This study consistently shows that
renal free-breathing BGS pCASL with retrospective motion compensation by image
registration improves tSNR, suggesting better reproducibility than non-BGS,
without compromising PWS. Overall, tSNR is higher in SS acquired images
compared with MS. Surprisingly, ASLReg outperforms FatReg even on the BGS data
with limited anatomical contrast. For both registration techniques, tSNR increased
and robustness of PWS improved. We anticipate that with more aggressive BGS image-based
motion compensation will require guidance by fat-images. FatReg could act similarly
for co-registration of the M0 image to BGS data, as was already performed in
this study.
Future work will focus on minimizing the phase interval to reduce
possible motion between ASL and fat-images. Additionally, incorporating complex
data subtractions will enable perfusion quantification with more aggressive BGS,
maximizing tSNR gain and due to successful FatReg free-breathing motion compensation,
alleviating patient cooperation for abdominal ASL. Acknowledgements
This work is part of the research programme Applied and Engineering
Sciences with project number 14951 which is (partly) financed by the Netherlands
Organisation for Scientific Research (NWO). We thank MeVis Medical Solutions AG
(Brehmen, Germany) for providing MeVisLab medical image processing and
visualization environment, which was used for image analysis.References
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