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DKI can early detect radio-insensitive human nasopharyngeal carcinoma xenograft in nude mice
Xiang Zheng1, Yunbin Chen1, Youping Xiao1, and Dechun Zheng1

1Fujian Provincial Cancer Hospital, Fuzhou, China

Synopsis

In order to evaluate feasibility of DKI sequence in early differentiating radio-insensitivity of nasopharyngeal carcinoma xenografts, Seventy-two nude mice were implanted with CNE-1(low radiosensitivity) and CNE-2(high radiosensitivity) and the xenografts were obtained. MRI scanning was performed after fractional irradiation. There are differences of the changes of DKI parameters (both D and K) between CNE-1 and CNE-2 before tumor volumes changed. Therefore, Both D and K can early (before volumes changed) distinguish radio-insensitive NPC xenografts from others.

OBJECTIVE

To explore the feasibility of DKI sequence in early differentiating radio-insensitivity of nasopharyngeal carcinoma xenografts in nude mice.

MATERIALS AND METHODS

Seventy-two nude mice were implanted with CNE-1(low radiosensitivity) and CNE-2(high radiosensitivity) nasopharyngeal carcinoma cells, and the xenografts were obtained. Then, the mice were underwent fraction irradiation separately. Nude mice of each cell line were randomly divided into non-irradiated group (G0), 10Gy group (G1), 20Gy group (G2), 30Gy group (G3) and 3 and 5 days after the whole dose irradiation group (G4, G5). DKI sequence was performed on each group. Volumes, parameter D and K were measured by two experienced radiologists double blindly. Student t test and receiver operating characteristic (ROC) curve analyses were included in our study. Significant level α was be chosen as 0.05.

RESULTS

The difference of volumes’ shrink rate between CNE-1&2 were firstly find in G2, with the shrink rate of 5.954% and 27.716% (p=0.032). D value decreased first (DG1, p=0.001) and then increased, K value increased first (KG1, p=0.001) and then decreased after irradiation in CNE-2, but not in CNE-1 xenografts (p>0.05). The AUC of DG1 and KG1 were 0.875 and 0.917, with sensitivity of 0.667 and 0.833, specificity of 1.000 and 1.000 respectively in the cutoff values 1.27×10-3mm2/s of parameter D and 0.88 of parameter K.

CONCLUSION

Both D and K can early (before volumes changed) distinguish radio-insensitive NPC xenografts from others. DG1 and KG1 may be the most useful parameters.

Key Words

Nasopharyngeal Carcinoma, Diffusion kurtosis imaging, Xenograft

Acknowledgements

No acknowledgement found.

References

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Figures

Figure 1 Changes of D and K value during fractional irradiation

Proc. Intl. Soc. Mag. Reson. Med. 26 (2018)
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