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DKI can early detect radio-insensitive human nasopharyngeal carcinoma xenograft in nude mice1Fujian Provincial Cancer Hospital, Fuzhou, China
Synopsis
In order to evaluate feasibility of DKI sequence in early differentiating radio-insensitivity of nasopharyngeal carcinoma xenografts, Seventy-two nude mice were implanted with CNE-1(low radiosensitivity) and CNE-2(high radiosensitivity) and the xenografts were obtained. MRI scanning was performed after fractional irradiation. There are differences of the changes of DKI parameters (both D and K) between CNE-1 and CNE-2 before tumor volumes changed. Therefore, Both D and K can early (before volumes changed) distinguish radio-insensitive NPC xenografts from others.
OBJECTIVE
To explore the feasibility of DKI sequence in early differentiating radio-insensitivity of nasopharyngeal carcinoma xenografts in nude mice.MATERIALS AND METHODS
Seventy-two nude mice were implanted with CNE-1(low radiosensitivity) and CNE-2(high radiosensitivity) nasopharyngeal carcinoma cells, and the xenografts were obtained. Then, the mice were underwent fraction irradiation separately. Nude mice of each cell line were randomly divided into non-irradiated group (G0), 10Gy group (G1), 20Gy group (G2), 30Gy group (G3) and 3 and 5 days after the whole dose irradiation group (G4, G5). DKI sequence was performed on each group. Volumes, parameter D and K were measured by two experienced radiologists double blindly. Student t test and receiver operating characteristic (ROC) curve analyses were included in our study. Significant level α was be chosen as 0.05.RESULTS
The difference of volumes’ shrink rate between CNE-1&2 were firstly find in G2, with the shrink rate of 5.954% and 27.716% (p=0.032). D value decreased first (DG1, p=0.001) and then increased, K value increased first (KG1, p=0.001) and then decreased after irradiation in CNE-2, but not in CNE-1 xenografts (p>0.05). The AUC of DG1 and KG1 were 0.875 and 0.917, with sensitivity of 0.667 and 0.833, specificity of 1.000 and 1.000 respectively in the cutoff values 1.27×10-3mm2/s of parameter D and 0.88 of parameter K.CONCLUSION
Both D and K can early (before volumes changed) distinguish radio-insensitive NPC xenografts from others. DG1 and KG1 may be the most useful parameters.Key Words
Nasopharyngeal Carcinoma, Diffusion kurtosis imaging, XenograftAcknowledgements
No acknowledgement found.References
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