We designed and optimized a dynamic double spin echo (DSE) acquisition scheme for hyperpolarized(HP) 13C metabolites dynamic diffusion weighted imaging(DWI). Compared to traditional DSE sequence, our sequence could provide higher SNR for longer dynamic imaging duration. Our studies on TRAMP mice indicate a dynamic change of HP lactate ADC over time, which might have potential to improve the assessment of aggressive cancers.
ss-DSE design
In previous work, we described the details about design our ss-DSE and compared to the ns-DSE[13]. According to Fig.1(1), the ss-DSE showed a smaller saturation effects than the ns-DSE sequence, and in (2), the optimized high bandwidth pulse had a smaller transition area (1.2mm) than the low bandwidth pulse (4.2mm).
In vivo study and acquisition scheme
In vivo data were acquired in transgenic adenocarcinoma of the mouse prostate(TRAMP)(n=2) on a 14T vertical imaging system (Agilent). All the HP 13C sample preparation steps were summed in our previous work[13]. 350μL HP pyruvate was injected over 15s via tail vein, and the sequences started at the end of the injection. The DSE sequence used a flyback EPI readout, with 10mm slice thickness and 2x2mm2 in-plane resolution. Based on prior ex vivo studies[8,9], the signal from HP lactate generated and exported from cells would last ~20s. Thus we chose our DWI acquisition to cover 20~30s. The temporal resolution was 3s, and in each timepoint HP pyruvate was acquired with 1 low b-value (50s/mm2) with 10° flip-angle and HP lactate was acquired with 3 different b-values (50, 500, and 1000s/mm2) with a constant 30° flip-angle. The acquisition scheme details are summarized in Fig.2.
Data analysis
Dynamic low b-value HP pyruvate and lactate images were used to calculate metabolic flux of HP 13C-pyruvate to lactate (kPL) and the overall decay rate. At each time point, three different b-value images were fitted to mono-exponential model pixel-by-pixel to calculate the apparent diffusion coefficient (ADC) map.
Results and Discussion
ns-DSE, ss-DSE performance comparison
For each study the experiment was performed twice, once with ss-DSE and the other with ns-DSE, using the same parameters for performance comparison. The dynamic HP 13C pyruvate and lactate low b-value images are compared in Fig.3. The overall decay rate of pyruvate and lactate was directly calculated without any corrections (e.g. for flip angle or T1 decay compensation). Both HP lactate and pyruvate decay rates with ss-DSE acquisition were lower than with ns-DSE, providing higher SNR at later time points. And, the estimated kPL was .094/s for ss-DSE, .071/s for ns-DSE in the tumor, also likely due to differences in saturation between the sequences.
Dynamic HP Lactate DWI
Fig.4 shows the ADC maps of ss-DSE based dynamic DWI. The ADC values we obtained are in good agreement with previous in vivo[7] and ex vivo studies[11,12]. The ADC histogram and average ADC for each time point (Fig. 5) showed a shift of the ADC values from ~0.3*10-3mm2/s at the end of injection to ~0.6*10-3mm2/s 9s after the end of injection. The shift of ADC values is consistent with lactate efflux during the experiment. Initially, most of the lactate transformed from pyruvate is intracellular with a lower ADC value, similar to cell studies[8] that measured an intracellular lactate ADC of ~0.2*10-3mm2/s. Furthermore, these studies measured extracellular lactate ADCs of ~0.6*10-3mm2/s which is similar to the largest ADC values measured. Thus we expect the ADC would increase over time due to lactate efflux from the intracellular to extracellular space.
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