We introduce and demonstrate a novel “k-t-segmented” gradient echo 3D-EPI variant for motion-robust, rapid quantitative susceptibility and R2* mapping at 3T. The combination of a versatile 2D-CAIPIRINHA EPI sampling (“k-segmentation”) and two complementing multi-echo options (“t-segmentation”) provides maximum time- and SNR-efficiency by acquiring exactly as many k-space lines per echo time as fit to the required R2* sampling without compromising spatial resolution. Offline averaging of multiple, rapid measurements (here: 6 averages, 6 echo times from 6.5-31.5ms, 52s/average) following optional, retrospective correction for motion and B0 drift, yields excellent quantitative whole-brain maps at 0.8mm isotropic resolution acquired in approximately 5 minutes.
A gradient-echo 3D-EPI sequence9,10 was extended by “true” multi-echo capability and an additional option to achieve a finer TE spacing than given by the ETL (Fig. 1A). In extension to traditional segmentation along the secondary phase encode direction (PE2), a flexible 2D-CAIPIRINHA k-segmentation of the EPI trajectory (Fig. 1B) was implemented to decouple the ETL from spatial resolution. Further vital modifications were: binomial-121 water excitation instead of typical fat-saturation, and performing only one EPI phase correction scan prior to each volume acquisition (minimize first TE). The following whole-brain protocol was performed on a MAGNETOM Prisma 3T scanner (Siemens, 80mT/m gradients, 64-channel head-neck coil, complex coil combination using body-coil as reference, online 2D-GRAPPA reconstruction): 264x264x176 matrix, 0.8mm isotropic, TE1-6=[6.5, 11.5, 16.5, 21.5 26.5, 31.5]ms (odd-numbered TEs acquired first), CAIPIRINHA 4x2(shift=1), partial Fourier 6/8x1, k-/t-segmentation: 7/2, 1.2ms echo spacing, 15° flip angle, TRvol=26s (52s for all TEs), 6 averages, TA=5:25min (incl. 13s preparation and FLASH autocalibration scans11).
Offline averaging of the complex images allows for retrospective motion correction (or scrubbing in case of excessive motion) and to correct for a B0 drift over time. The latter was done here using the voxel-wise autocorrelation method12 across all averages to estimate a temporal field slope. Following complex averaging, an optional adaptive non-local means (NLM) denoising filter13 was applied to the corresponding real and imaginary parts.
Monoexponential R2* maps have been computed via linear fitting of the logarithm of the averaged magnitude images according to TE1-6. The averaged phase maps have been unwrapped14, divided by 2πTE and summarized to a single frequency map using optimized weights15. The frequency map was corrected for background contributions using V-SHARP16,17 and supplied to homogeneity enabled incremental dipole inversion (HEIDI)18 to reveal quantitative susceptibility maps.
1 Deistung A, Schweser F, Reichenbach JR. Overview of quantitative susceptibility mapping. NMR Biomed 2016;(May). https://doi.org/10.1002/nbm.3569.
2 Sati P, Gelderen P van, Silva AC, Reich DS, Merkle H, De Zwart JA et al. Micro-compartment specific T2* relaxation in the brain. Neuroimage 2013;77:268–78. https://doi.org/10.1016/j.neuroimage.2013.03.005.
3 Liu C, Li W, Johnson GA, Wu B. High-field (9.4T) MRI of brain dysmyelination by quantitative mapping of magnetic susceptibility. Neuroimage 2011;56(3):930–8. https://doi.org/10.1016/j.neuroimage.2011.02.024.
4 Langkammer C, Krebs N, Goessler W, Scheurer E, Ebner F, Yen K et al. Quantitative MR Imaging of Brain Iron: A Postmortem Validation Study. Radiology 2010;257(2):455–62. https://doi.org/10.1148/radiol.10100495.
5 Langkammer C, Schweser F, Krebs N, Deistung A, Goessler W, Scheurer E et al. Quantitative susceptibility mapping (QSM) as a means to measure brain iron? A post mortem validation study. Neuroimage 2012;62(3):1593–9. https://doi.org/10.1016/j.neuroimage.2012.05.049.
6 Sun H, Wilman AH. Quantitative susceptibility mapping using single-shot echo-planar imaging. Magn Reson Med 2014;00:1–7. https://doi.org/10.1002/mrm.25316.
7 Langkammer C, Bredies K, Poser Ba, Barth M, Reishofer G, Fan AP et al. Fast quantitative susceptibility mapping using 3D EPI and total generalized variation. Neuroimage 2015;111:622–30. https://doi.org/10.1016/j.neuroimage.2015.02.041.
8 Breuer FA, Blaimer M, Mueller MF, Seiberlich N, Heidemann RM, Griswold MA et al. Controlled aliasing in volumetric parallel imaging (2D CAIPIRINHA). Magn Reson Med 2006;55(3):549–56. https://doi.org/10.1002/mrm.20787.
9 Poser BA, Koopmans PJ, Witzel T, Wald LL, Barth M. Three dimensional echo-planar imaging at 7 Tesla. Neuroimage 2010;51(1):261–6. https://doi.org/10.1016/j.neuroimage.2010.01.108.
10 Stirnberg R, Huijbers W, Brenner D, Poser BA, Breteler M, Stöcker T. Rapid whole-brain resting-state fMRI at 3 Tesla: Efficiency-optimized three-dimensional EPI versus repetition time-matched simultaneous-multi-slice EPI. Neuroimage 2017;163(August):81–92. https://doi.org/10.1016/j.neuroimage.2017.08.031.
11 Ivanov D, Barth M, Uludağ K, Poser BA. Robust ACS
acquisition for 3D echo planar imaging.
In Proc Intl Soc Mag Reson Med 23, 2015
12 Ahn CB, Cho ZH. A new phase correction method in NMR imaging based on autocorrelation and histogram analysis. IEEE Trans Med Imaging 1987;6(1):32–6. https://doi.org/10.1109/TMI.1987.4307795.
13 Manjón JV, Coupé P, Martí-Bonmatí L, Collins DL, Robles M. Adaptive non-local means denoising of MR images with spatially varying noise levels. J Magn Reson Imaging 2010;31(1):192–203. https://doi.org/10.1002/jmri.22003.
14 Abdul-Rahman HS, Gdeisat M a, Burton DR, Lalor MJ, Lilley F, Moore CJ. Fast and robust three-dimensional best path phase unwrapping algorithm. Appl Opt 2007;46(26):6623–35. https://doi.org/10.1364/AO.46.006623.
15 Wu B, Li W, Avram AV, Gho SM, Liu C. Fast and tissue-optimized mapping of magnetic susceptibility and T2* with multi-echo and multi-shot spirals. Neuroimage 2012;59(1):297–305. https://doi.org/10.1016/j.neuroimage.2011.07.019.
16 Schweser F, Deistung A, Lehr BW, Reichenbach JR. Quantitative imaging of intrinsic magnetic tissue properties using MRI signal phase: An approach to in vivo brain iron metabolism? Neuroimage 2011;54(4):2789–807. https://doi.org/10.1016/j.neuroimage.2010.10.070.
17 Wu B, Li W, Guidon A, Liu C. Whole brain susceptibility mapping using compressed sensing. Magn Reson Med 2012;67(1):137–47. https://doi.org/10.1002/mrm.23000.
18 Schweser F, Sommer K, Deistung A, Reichenbach JR. Quantitative susceptibility mapping for investigating subtle susceptibility variations in the human brain. Neuroimage 2012;62(3):2083–100. https://doi.org/10.1016/j.neuroimage.2012.05.067.