An inversion recovery bSSFP measurement allows to generate a spectrum of the apparent relaxation time T1* and hence to identify multiple components in a voxel. However, it is not possible to extract unambiguous T1 and T2 information for each individual component. Here, we demonstrate that this limitation can be overcome by an additional bSSFP measurement without inversion pulse. Additive and subtractive combinations of the measured signal courses provide enough information for assigning unambiguous T1, T2 and proton density values to each component. In that way, 2D T1-T2 correlation spectra can be generated voxel-wise in a very time-efficient manner.
Assuming
a single tissue component, quantitative T1, T2 and PD
values can be simultaneously obtained from an IR bSSFP measurement, when the
initial signal after inversion (S0), the steady-state signal (Sstst)
and T1* are determined. Additionally, multiple components can be
identified by analyzing the IR bSSFP signal course using the inverse Laplace
transform. However, the spectral amplitudes of the resulting T1*
spectrum depend on the sum of S0 and Sstst and hence it
is not possible to extract T1 and T2 information for an
individual T1* peak. To overcome this limitation, two bSSFP
measurements can be performed,
one with an inversion pulse and one without an inversion pulse prior to the bSSFP
readout.5 Both signals approach the same steady-state but from a
different initial signal. The added signals depend only on Sstst and
T1*:
$$S_{add}(t)=2·S_{stst}·(1-exp(\frac{-t}{T_1^*})) $$
The subtracted signals depend only on S0 and T1*:
$$S_{sub}(t)=2·S_0·exp(\frac{-t}{T_1^*}) $$
T1* spectra can be generated from Sadd(t) and Ssub(t) as shown in Fig. 1. The areas under the peaks of the resulting T1* spectra represent S0 and Sstst respectively and are used to calculate T1, T2 and PD for each peak. The result can then be plotted in a 2D correlation spectrum (Fig. 2).
Phantom measurements were performed on a 3T MRI system to validate the principle of this technique. The phantom contained four different components - pure water, water with contrast agent, and sunflower oil with two specific chemical substances. 2048 echoes were acquired without phase encoding (TR = 4.6 ms, excitation angle = 60°). Spatially resolved reference measurements for T1 and T2 were performed using an inversion recovery spin-echo based sequence with different inversion times for T1 estimation and multiple spin-echo experiments with varying echo times for the calculation of T2.
In vivo experiments with 2048 radial projections were performed with a projection increment of 38.98° (TR = 5.0 ms, excitation angle = 40°). This tiny golden angle reduces eddy currents and provides sufficient k-space information for a narrow sliding window reconstruction with a high temporal fidelity.6 In total 408 images per measurement were reconstructed using the T1* shuffling method.4,7
T1* spectra were generated by applying the inverse Laplace transform on the added as well as the subtracted signals and T1, T2 and PD were calculated for each appearing peak.
The phantom experiment shows an excellent agreement with the reference measurements. Fig. 3 illustrates the spectra from the subtracted and added signals as well as the resulting T1/T2 correlation spectrum. In vivo results are shown in Fig. 4 and highlight the added and subtracted spectra from three exemplary voxels obtained by the inverse Laplace transform. The resulting T1/T2 pairs in the correlated spectra indicate e.g. myelin (T1* ≈ 100 ms), white matter (T1* ≈ 400 ms), gray matter (T1* ≈ 550 ms) and the cerebrospinal fluid (CSF) (T1* ≈ 3300 ms) in the expected brain areas.
Deviations from literature T1 and T2 values may occur due to B0 inhomogeneities and inaccurate flip angle information. Furthermore, deviations from the ideal radial trajectory may alter the steady-state signal and hence lead to a mismatch between the positions of the individual peaks of the combined T1* spectra.
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