Willy Gsell1, Uwe Himmelreich1, Cindy Casteels2, Christophe M. Deroose2, Antonio J. Gonzalez3, Albert Aguilar3, Carlos Correcher4, Emilio Gimenez4, Cesar Molinos4, Ramiro Polo4, Thorsten Greeb5, Ralph Wissmann5, Sven Junge5, and Jose M. Benlloch3
1Biomedical MRI, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium, 2Nuclear Medicine, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium, 3i3M, Valencia, Spain, 4Oncovision, Valencia, Spain, 5Bruker Biospin, Ettlingen, Germany
Synopsis
We
designed a novel PET insert based on monolithic LYSO crystals. From our first
evaluation, we can conclude that sub-millimeter detector spatial resolution,
combined with accurate photon DOI determination, make it possible to acquire high
resolution reconstructed images. This enables us now to combine simultaneously
high resolution and sensitivity PET with high field preclinical MRI to extract simultaneously
complex data from anatomical to molecular information and to dynamically follow
non-invasively animal models of different pathologies with no compromise in
performance of each imaging modality.
Introduction
Positron Emission Tomography
(PET) is a very sensitive molecular imaging modality, but it suffers from a lack
of anatomical information. The combination of PET with magnetic resonance
imaging (MRI) provides the ideal solution by having soft tissue contrast,
multi-functional read-outs (MRI-diffusion, perfusion, dynamic contrast
enhancement) and does not add additional exposure to ionizing x-ray radiation
1-5,
as Computed Tomography scanner do. However the design of a high resolution PET
insert for preclinical imaging has to overcome several challenges due to the
high static magnetic field, the fast switching of the gradient coils and
potential interaction of the radiofrequency field with PET electronics
6.
We designed a novel PET insert based on
monolithic LYSO crystals and high density arrays of SiPMs. The aim of this work
was thus to characterize the performance of such a system for simultaneous
PET-MRI acquisitions.
Methods
The
PET system formed by 8 detectors has been tested inside the Bruker BioSpec 70/30
USR with a magnetic field of 7 T (Figure 1). The MR system was equipped with a
BGA 20S-HP gradient coil. The inner and outer diameters of the PET scanner
(insert) are 114mm and 198mm, respectively, fitting inside the MR gradient. Quadrature
birdcage coils were used for all experiments (86mm inner diameter for phantom
and rat studies and 40mm for mouse imaging). For the RF shielding, we
implemented Carbon Fiber structures with tubular shape, surrounding the PET
electronics. Fast Spin echo and EPI sequences were tested simultaneously with
the PET insert. We assessed potential eddy currents induced by fast switching
of the gradient, field homogeneity through B0 maps and PET/MR image
quality (resolution, SNR, image homogeneity etc.). For PET characterization, we
followed the NEMA protocol for sensitivity, and use mini-Derenzo like phantom
(filled with 150uCi of 18F-FDG) for estimation of resolution and
image quality. For all tests, PET data were reconstructed using Maximum
Likelihood Estimation Method (MLEM) with either voxel size of 0.5 or 0.29mm3
and at least 12 iterations. Several animal models (mouse glioma, mouse
stroke, xenografts in mice, rat heart, see Figures) were evaluated to provide
real in-vivo data for the quality assessment of the simultaneous PET/MRI
acquisitions.
Results
We
tested different RF pulses (20ms and 630W, with 51us and 1ms duration) and MRI
sequences (RARE, EPI, etc…) without observing PET degradation of the PET image
quality or eddy currents that could produce a sub-optimal MR performance. The
FieldMap sequences showed in the present study no change in the B0 field with a
55mm spherical phantom when the PET insert was inside the MR scanner (SNR
variation with/without PET <6%). Both RARE and EPI sequences showed ghost
levels of about 2.4 to 3.6%. The PET geometry and performance were almost
identical to the current in-line Albira Si system6. Sub-millimeter image
resolution (between 0.9 and 0.7mm) and homogeneous-FOV spatial resolution were
reached, as shown in Fig.2 and Fig.3. The sensitivity for the one-ring PET,
following the NEMA protocol, was determined to be beyond 3.5%. In-vivo evaluation demonstrated the addedd value
of using simultaneously high resolution PET and MRI (Fig. 4). For example, a glioma
as small as 0.6mm3 were visualized on the MRI and small SUV
differences with contralateral side were demonstrated by PET. Dynamic data were
acquired using cardiac PET and MRI acquisition in the rat heart.
Discussion and Conclusion
The design of a first prototype of a
small animal PET insert is finalized and was successfully tested within a 7T
MRI (Bruker Biospec). Typical MR image sequences for anatomical and functional
imaging did not affect the PET performance. In-vivo experiments demonstrated
the benefit of such combination in small animal models.
PET detector spatial
resolution nearing 1mm, combined with accurate photon DOI determination, make
it possible to return high resolution reconstructed images visualizing the 750um
rods of the micro Derenzo-like phantom. This enables us to combine high
resolution and sensitivity PET with high-field preclinical MRI to extract
simultaneously complex data from anatomical to molecular information and to
dynamically follow non-invasively animal models of different pathologies.
Acknowledgements
MRI and PET imaging systems were founded by the Hercules foundation (PI: U. Himmelreich) and Stichting tegen Kanker (PI: C. Deroose)References
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