A novel quantitative imaging method based on steady-state signals was proposed to simultaneously resolve MR-related parameters. A special, two-flip angle acquisition was implemented to account for the fluctuations in B1+ field. Furthermore, we developed a motion-resistant sampling scheme to lessen the impact of motion on steady-state signal for in vivo brain scans. A 3D brain imaging that extracts main MR-related parameters such as T1, T2, T2*, M0, B0, and B1+ was performed here in less than 12 minutes.
The method is based on a multi-pathway multi-echo pulse sequence (Fig. 1a). It is a 3D steady-state sequence that samples three pathway signals at three different echo times. Two separate scans were performed, with different scaling for the RF waveform (Fig. 1a, gray dashed line, N×α1). An index notation of the form (scan, echo, pathway) was employed in Fig. 1a. Because the larger flip angle scan involved only a small central k-space region, it contributed very little to the overall scan time.
Signal pathways naturally get formed anytime a steady-state (i.e., no RF spoiling) sequence is employed3, see Fig. 1b. But typically, a pulse sequence would sample only one of these pathways. On the other hand, some sequences may sample two4 or as many as three5 pathways. A balanced steady-state (bSSFP) sequence actually samples them all, but in a form where they were first summed and overlapped. The pulse sequence used here is very similar to that employed in TESS5, except for the fact that each one of three acquired pathways gets sampled at a couple different echo times. In Fig. 1b, magnetization states F have a superscript –/+ that means just before/after an RF pulse, and a subscript for pathway number.
The evolution of magnetization states can be mathematically tracked using the equations and tools introduced by Hennig3, leading to a new set of equations solved here for T1, T2, T2*, M0, B0, and B1+. These equations are listed in Fig. 2: Eq. 1 was solved for R2 and R2′ (whereby T2 = 1/R2 and T2* = 1/ (R2+ R2′)), Eq. 2 for the flip angle α1 (and thus B1+), Eq. 3 for T1, and Eq. 4 for M0. Equations 2-4 used definitions from Eq. 5, and Eq. 1 was the same as that also employed in Cheng et al6. A flowchart for the reconstruction process is presented in Fig. 3a.
Scanning was performed on a 3.0T system (Siemens Trio) with a 12-element head matrix. Our 3D sequence provided volumetric parameter mapping, and a series of spin-echo images were acquired at one given 2D location within this volume, to provide a reference standard for T1 and T2. Scans were performed on an fBIRN gel phantom and in vivo on four healthy volunteers (following informed consent using an IRB-approved protocol). To reduce motion sensitivity in human scans, a sampling scheme inspired from the PROPELLER method7 was implemented in the ky-kz plane, to oversample the central k-space region (Fig. 3b). The overall acquisition time was 11m24s, only 40s of which was for the large flip angle acquisition (FOV = 192×192×192mm3, TR = 25ms, nominal flip angles α1/α2 = 15°/330°, matrix size = 192×160×160). One extra global fit parameter β was introduced, as in N = β×α1/α2.
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Fig. 5 Parameters maps are shown for one slice (out of 192) from one of four volunteers. More specifically, T2, T2*, f0, the flip angle, T1 and M0 are shown in (a) through (f), respectively.