Proton density (PD) measurements derived from single Spin Echo and PnP-MRF, which is a fingerprinting protocol that enables simultaneous estimation of the excitation field (B1+), were validated using the ISMRM/NIST MRI system phantom that contains known D2O/H2O solutions at 14 different concentrations. We also expanded the PnP-MRF protocol by exploiting the symmetry between B1+ and the receive field, B1-, produced by a quadrature body coil at 3 Tesla in order to remove all B1 contribution from our PD. Ultimately, all methods showed remarkable correlation with the known water fraction from NIST samples (Pearson’s rho>0.99).
We used our Siemens Magnetom PRISMA (Siemens, Erlangen, Gremany) in combination with the standard 20-channel head coil, to image a single axial slice through the NIST phantom7. This phantom offers an array of 14 spheres with various concentrations of D2O/H2O to create a range of different$$$\,PD\,$$$values.
The Plug-and-Play MR Fingerprinting (PnP-MRF) sequence2 was used to generate a$$$\,PD$$$-map (total scan time 8-min). In addition to $$$PD$$$, $$$T_2$$$, and, $$$T_1$$$ PnP-MRF also enables the simultaneous estimation of the excitation field ($$$B_1^+$$$). Although this removes possible $$$B_1^+$$$ bias effects from the $$$T_1$$$ and $$$T_2$$$ measurements, the$$$\,PD\,$$$remains proportional to the receive field ($$$B_1^-$$$). A rigorous measurement of the field remains extremely challenging8. Instead, clinical systems typically use the large body coil as a reference to estimate the fields produced by an array of local receivers. This step, referred to as pre-scan normalize, mitigates the strong spatial variations in the fields of a close fitting receive array, leaving only a small variations corresponding to the residual variations in the receive field of the body coil. In this work we expanded on the pre-scan normalize method by exploiting the symmetry between and fields produced by a quadrature body coil at 3 Tesla9,10. After generating parametric maps from PnP-MRF, we used the estimated field to correct the$$$\,PD\,$$$maps.
$$\,\,\,\,\,\,\,\,\,\,\,\,PD=\frac{PD_{pnp-mrf}}{\alpha\,|B_1^+|}\,\,\,\,\,\,\,\,\,\,\,\,(1)$$
where $$$\,\alpha=|BC|/|B_1^+|$$$ and $$$BC\,$$$is the signal from the body coil.
In addition, we also acquired a series of single Spin Echo (SE, Total scan time 8 hours). Signal decay from SE was modeled as a function of echo time (TE) using an exponential model:$$$\,S(TE)=PD\text{exp}(-TE/T_2)$$$, with 2 parameters:$$$\,PD\,$$$and$$$\,T_2\,$$$. In this case the product inline pre-scan normalize feature and the online Siemens reconstruction tools were used to reconstruct the data. A complete overview of all sequence parameters can be found in Table1.
The accuracy of these methods was quantified by comparing water fraction ($$$\phi$$$) in each of the 14 spheres to the ground truth fraction provided by NIST. The signal within each sphere was normalized by the signal from the sphere containing 100% H2O: $$\,\,\,\,\,\,\,\,\,\,\,\,\phi=\frac{PD_N}{PD_{100\%}}\,\,\,\,\,\,\,\,\,\,\,\,(2)$$
Histogram analysis was used to evaluate the signal variability on normalized images for the water outside of the D2O/H2O spheres, where the proton density is expected to be uniform. The width of the histogram serves as a marker for residual inhomogeneity in the image.
[1] Ma D, Gulani V, Seiberlich N, Liu K, Sunshine JL, Duerk JL, Griswold MA. Magnetic resonance fingerprinting. Nature 2013;495(7440):187-192.
[2] Cloos MA, Knoll F, Zhao T, Block KT, Bruno M, Wiggins GC, Sodickson DK. Multiparametric imaging with heterogeneous radiofrequency fields. Nature communications 2016;7:12445.
[3] Jiang Y, Ma D, Keenan KE, Stupic KF, Gulani V, Griswold MA. Repeatability of magnetic resonance fingerprinting T1 and T2 estimates assessed using the ISMRM/NIST MRI system phantom. Magnetic Resonance in Medicine:n/a-n/a.
[4] Venn M, Maroudas A. Chemical composition and swelling of normal and osteoarthrotic femoral head cartilage. I. Chemical composition. Annals of the Rheumatic Diseases. 1977;36(2):121-129.
[5] Fieremans E, De Deene Y, Delputte S, Ozdemir MS, Achten E, Lemahieu I. The design of anisotropic diffusion phantoms for the validation of diffusion weighted magnetic resonance imaging. Physics in medicine and biology 2008;53(19):5405-5419. [6] Lemberskiy G, Baete S, Cloos M, Novikov D, Fieremans E. Validation of S/V derived from Oscillating Gradient Spin Echo on a clinical scanner using anisotropic fiber phantoms. Proceedings of the International Society of Magnetic Resonance in Medicine 24, Singapore;.Diffusion at the Cutting Edge. 0013, 2016.
[6] (http://collaborate.nist.gov/mriphan- toms/bin/view/MriPhantoms/MRISystemPhantom). (http://collaborate.nist.gov/mriphan- toms/bin/view/MriPhantoms/MRISystemPhantom).
[7] Quantitative (qMRI) ISMRM System phantom from NIST. (http://collaborate.nist.gov/mriphantoms/bin/view/MriPhantoms/WebHome)
[8] Sodickson D, Alon L, Deniz M C, Novikov S D, Generalized Local Maxwell Tomography for Mapping of Electrical Property Gradients and Tensors. Proceedings of the International Society of Magnetic Resonance in Medicine 21, Salt Lake City, USA, 4175, 2013.
[9] Voigt T, Katscher U, Doessel O. Quantitative conductivity and permittivity imaging of the human brain using electric properties tomography. Magnetic Resonance in Medicine 2011;66(2):456-466
[10] van Lier ALHMW, Raaijmakers A, Voigt T, Lagendijk JJW, Luijten PR, Katscher U, van den Berg CAT. Electrical Properties Tomography in the Human Brain at 1.5, 3, and 7T: A Comparison Study. Magnetic Resonance in Medicine 2014;71(1):354-363.