Higher Angular Resolution Diffusion Imaging (HARDI) methods, such as Diffusion Spectrum Imaging (DSI[1]) and multishell Q-ball imaging[2] are robust tools for non-invasive imaging of in vivo white matter architecture. These methods capture the complex intravoxel crossings [1,3] in Orientation Distribution Functions (ODFs). A key step to use these ODFs in tractography algorithms is the correct identification of the fiber directions in each voxel. Limited angular resolution of the acquisition and intrinsic ODF peak width [4] make it however difficult to correctly estimate fiber directions accurately in the case of small crossing angles [5,6]. Most proposed methods fail to detect crossing angles less than 40° [5,6]. Even deconvolving the ODFs with a Fiber Response Function fails to reliably detect crossing angles smaller than 30°[7].
Here we propose a new approach inspired by the recent success of fingerprinting approaches in quantitative MRI imaging[8,9]. We generate a library of ODF-fingerprints and identify the fiber directions of ODFs by assessing similarity with the library of fingerprints (Figure 1). We demonstrate this method on both simulated and in vivo measured ODFs.
A library of ODF-fingerprints is generated by first simulating diffusion weighted signals for a number of possible fiber combinations (up to 2 fibers; main fiber along the Z-axis, angles of subsequent fibers sampled on a 642-point tessellation of the unit sphere; fiber FA ranging from 0.4 to 0.8 in steps of 0.1; assumption of cylindrical fibers ($$$\lambda_2=\lambda_3$$$) with a simple diffusion tensor model; fiber bundle volume relative to the voxel size ranging from 0 to (1-water component) in steps of 0.1; 10% water component; ADC=1.0mm$$$^2$$$/s) on a Radial Diffusion Spectrum Imaging grid (236 q-space samples on four shells, b=1000,2000,3000,4000s/mm$$$^2$$$ (simulations), and b=200,1500,2750,4000s/mm$$$^2$$$ (in vivo)[10]). The ODF-fingerprints are then subsequently calculated from the simulated diffusion weighted signals. For each measured ODF-trace, we find the matching ODF-fingerprint by searching for the ODF-fingerprint with the largest dot-product[8,9]. The size of the fingerprint-library is reduced by rotating the maximum value of ODF-traces to the Z-axis before matching.
In an in-house simulation, ODF-samples are simulated as above, but with random fiber directions. Rician noise is added where necessary. Additionally, a crossing fiber-phantom is simulated with the Phantomas-software[11] (composite hindered and restricted diffusion model[12]). In vivo DSI acquisitions are acquired on a 3T clinical scanner (Prisma, Siemens, Erlangen; 20ch head coil; bmax=4000, TR/TE=2600/114ms, 60 slices, 220mm FoV, 2.2x2.2x2.2mm resolution, 2x in slice GRAPPA, PF6/8, SMS4; healthy male volunteer, 29 y/o). The images are denoised [13] and corrected for susceptibility, eddy currents and subject motion using eddy from the FSL Library [14]. RDSI reconstructions, incorporating variable sample density correction, were performed offline using custom-made software (Matlab, Mathworks) and displayed with DSIStudio [15].
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