Coronary artery endothelial function (CEF) could historically only be measured with invasive catheterization-based testing. Recently, 2-dimensional cine MRI in combination with isometric handgrip exercise was introduced to quantify CEF noninvasively. However, the MRI technique only assesses CEF at one or two locations in an artery and does not allow assessment of regional heterogeneity present throughout diseased vessels. The aim of this work was to develop high-resolution isotropic 3-dimensional cine coronary MRI that enables measures of coronary lumen area along the entire proximal and mid segments of the coronary arteries in a single breath-hold, a pre-requisite for future 3-dimensional CEF measurements.
Acquisition: All data were acquired on a 3T scanner (Philips Achieva) with a 32-channel cardiac coil. The sequence was tested in a resolution phantom (with 4.5mm diameter rods) and six datasets were acquired along the right coronary arteries of 3 subjects. Variable-density spiral interleaves (Figure 1A) were acquired for ~20s while rotating consecutive interleaves by the golden-angle (137.508°). Pseudo-randomized phase-encoding was performed in slice direction with a higher density at the k-space center (Figure 1B). The ECG-signal was recorded and used for retrospective data binning to 20 cardiac phases. Example kz-ky sampling pattern for two cardiac phases after retrospective binning is shown in Figure 1C. Imaging parameters were: field-of-view = 300x300x40mm3, slice-oversampling = 25%, water selective spectral-spatial excitation with an excitation angle = 20°, isotropic voxel sizes from 1.1 to 1.3mm, repetition time / echo time = 22 / 2.2 ms. The 3D volume was planned orthogonal to an axial, low-resolution whole-heart coronary MR angiogram covering the proximal and mid right coronary artery. All data were acquired in single 20s breath-holds.
Reconstruction: Data were reconstructed off-line on a MacPro using GPI.16 The GRASP14 reconstruction, originally designed for 2D radial trajectories, was adapted for 3D spiral trajectories and is referred to as GASSP reconstruction. It iteratively minimizes the combination of parallel-imaging data consistency and temporal total-variation based sparsity constraints. After reconstruction, each image slice was deblurred with a single frequency to correct the area around the coronary artery only.17
Data Analysis: Cross-sectional area along the coronary arteries was quantified from the 3D data using a semi-automated tool, which performs centerline tracking and a full-width-at-half-maximum (FWHM) segmentation between two manually selected endpoints.18 Results were averaged over 4 mm segments throughout each artery.
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