The modified Look Locker (MOLLI) sequence is the most widely used myocardial T1 mapping approach. In this 2D sequence, several T1-weighted images are acquired within a breath hold and used to create a T1 map. However, some patients are unable to sustain stable breath-holds which generates 3D motion between the T1-weighted images. While image registration can potentially be used to correct for in-plane motion, through-plane motion cannot be corrected and may introduce bias in T1 estimates. In this study, we sought to develop and assess a free breathing MOLLI sequence with real-time slice tracking and non-rigid image registration to reduce in-plane and through-plane motion effects.
Proposed sequence: A prototype MOLLI sequence was developed by adding a navigator restore pulse after each inversion pulse and a navigator echo readout before each image acquisition. Real-time slice tracking was implemented based on the navigator echo and a tracking factor of 0.6 to reduce through-plane motion. Non-rigid image registration was performed online after acquisition to reduce in-plane motion. T1 fitting was performed using the conventional approach for MOLLI (3-parameter model fit followed by Look Locker correction5).
Experimental validation: All imaging was performed using a 1.5T Magnetom Aera scanner (Siemens Healthcare, Erlangen, Germany). All MOLLI T1 mapping scans used a bSSFP readout (TR/TE=2.5ms/1.2ms, flip angle=35°, FOV=360×306mm2, voxel size=2.2×2.5mm2, slice thickness=8mm, slice number=3, bandwidth=1085Hz/Px, GRAPPA acceleration factor=2, partial Fourier=0.87, MOLLI scheme: 5-(3)-3). The MR-navigator was positioned on the dome of the diaphragm of each subject.
Study #1: Assessment of T1 mapping reproducibility. N=6 volunteers were recruited for a cardiac MRI study. Each volunteer was imaged twice using the conventional breath-hold (BH) MOLLI sequence and twice using the proposed free breathing (FB) MOLLI sequence. Both BH and FB sequences used the same imaging parameters described above. Native myocardial T1 measurements and reproducibility (absolute T1 time difference between the two scan repetitions) were evaluated. Both metrics were calculated for each of the 16 AHA myocardial segments as well as over all myocardial segments.
Study #2: Clinical feasibility. Three patients referred for assessment of cardiomyopathies were recruited. The feasibility of FB MOLLI was demonstrated in 3 patients referred to CMR for assessment of cardiomyopathy. Each patient was imaged using both BH and FB MOLLI sequences.
Study #1: Figure 1 shows representative native T1 maps acquired in one healthy volunteer using both the conventional (BH) and proposed (FB) MOLLI techniques. Excellent and similar image quality was obtained with both techniques. Figure 2 shows native myocardial T1 measurements and reproducibility obtained over all subjects. In average over all myocardial segments, native myocardial T1 times were 995±24ms using FB MOLLI and 974±16ms using BH MOLLI (p=0.01). There was no statistical significant differences between both techniques in terms of myocardial T1 reproducibility when measured in averaged over all myocardial segments (8±8ms using FB MOLLI vs. 8±7ms using BH MOLLI, p=0.46). There was no statistical differences between both techniques in terms of myocardial T1 reproducibility per myocardial segment (23±8ms using FB MOLLI vs. 18±6ms using BH MOLLI, p=0.22).
Study #2: Figure 3 shows representative native T1 maps obtained in one patient. Both conventional BH and proposed FB MOLLI sequences provided excellent and similar T1 map quality.
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