Clinical translation of cardiac diffusion tensor MRI has been challenging because of the sensitivity to bulk motion and thus, low success rates of scans. Current techniques require either high gradient systems (>40 mT/m) or excess breath holding ( >10 breath holds / slice) to acquire motion free cardiac DT-MRI. We propose a cardiac DT-MRI technique optimized for clinical translation and aimed at achieving high success rates in subjects with high and variable heart rate and high bold-to-mass index under free breathing conditions. Results in subjects with high BMI and variable HR yielded success rates > 90%.
PULSE SEQUENCE DESIGN: The proposed DT-MRI technique uses an optimized M2 spin echo1 reduced field-of-view (FOV) single shot echo-planar-imaging (EPI) (Figure 1). M2 spin echo diffusion encoding was used to address both cardiac and respiratory bulk motion with regard to diffusion motion sensitivity leading to possible signal drop out. ECG gating at end systole was prescribed to address variable HR and retrospective non-rigid motion correction (MIRT4) to address respiratory motion shifts between diffusion weighted directions and averages during free breathing acquisition (Figure 2). Reduced FOV EPI via cross-pair spin echo addresses high BMI with only the heart being selectively imaged instead of the entire FOV. Additionally, EPI at high field (≥3T) is prone to the increased main field inhomogeneity and requires a short readout to avoid severe susceptibility artifacts and geometric distortion5. Both partial (5/8) Fourier in the phase encode and reduced field-of-view (FOV; 33%) was used to reduce the EPI echo train by a factor ~5.
IN VIVO IMAGING: Eight (n = 8) volunteers without any history of cardiovascular disease but exhibited variable HR during DT-MRI scan (SD: 12 +/- 3 beats-per-minute (BPM); Range: [58 95] BPM) and high BMI (30 +/- 4.5, [25 – 36]) were scanned using the proposed free breathing M2 DT-MRI sequence (TR=4RR, TE=85ms, EPI temporal footprint = 15ms, 3 short axis, 2.3x2.3x10mm, 1b0 + 6 directions, 8 averages, scan time: 2.5 min) on a clinical wide bore (70cm) 3T scanner (Skyra, Siemens Healthcare, Erlangen, Germany). A scout mode that continuously acquired a single DWI at varying trigger delays preceded the DT-MRI scan (Figure 2a). Once a trigger was quickly found yielding motion free images, then that trigger was used for the entire DT-MRI scans for all averages and directions.
IMAGE ANALYSIS: Diffusion tensors were fitted voxelwise after non-rigid co-registration was applied to correct respiratory motion assuming a mono-exponential fit using custom software developed in Matlab. Mean diffusivity (MD), fractional anisotropy (FA), and helix angle (HA) were calculated from the reconstructed tensor. Success rates were determined by the number of DWI that did not exhibit greater > 10% signal loss (apparent diffusion coefficient > 3.0 um^2/ms; labeled as “motion corrupted” in figure 2c) out of all DWI acquired (6 directions x 8 averages = 48). Success rates were plotted against HR, HR variability (standard deviation of HR during scan), BMI, and age.
1. Nguyen C, Fan Z, Xie Y, Pang J, Speier P, Bi X, Kobashigawa J, Li D. In vivo diffusion-tensor MRI of the human heart on a 3 tesla clinical scanner: An optimized second order (M2) motion compensated diffusion-preparation approach. Magn. Reson. Med. 2016 Aug 1.
2. Mekkaoui C, Reese TG, Jackowski MP, Bhat H, Sosnovik DE. Diffusion MRI in the heart. NMR Biomed. 2015 Oct 20.
3. Stoeck CT, Deuster von C, Genet M, Atkinson D, Kozerke S. Second-order motion-compensated spin echo diffusion tensor imaging of the human heart. Magn. Reson. Med. 2015 May 28.
4. Myronenko A, Song X. Intensity-based image registration by minimizing residual complexity. IEEE Trans. Med. Imaging. 2010 Nov;29(11):1882–91.