The free water elimination (FWE) model fitting problem is inherently ill-conditioned, leading to the need for solutions that can avoid or deal with these kinds of fitting problems. In this work, we evaluate a model extension to the FWE model that exploits the T2-relaxation properties and subsequently leads to a well-posed fitting problem that can be easily solved using standard estimation techniques.
The free water elimination (FWE) diffusion model accounts for partial volume effects that occur when voxels in diffusion tensor imaging (DTI) volumes contain both a tissue and a free water compartment1-2. A major downside of FWE is that the model fitting problem is ill-conditioned, or, for isotropic diffusion, even ill-posed3-4. Therefore, FWE has previously been tackled with advanced parameter estimation techniques that incorporate, e.g., regularization2 or statistical priors5-6. These methods usually succeed in stabilizing the model fit but, as a trade-off, impose model assumptions that are likely to bias the results.
In this work, we exploit that the T2 relaxation times of white matter and cerebrospinal fluid are very different, i.e. approximately 70ms7 and 500ms8, respectively. By accounting for the associated TE dependency of the signal decay, the model parameters can be estimated more precisely, accurately, and robustly.
FWE models the diffusion weighted signal $$$S_i$$$ as:
$$S_i=S_0\left(\left(1-f\right)e^{-b_ig^T_iDg_i}+fe^{-b_id}\right)\hspace{1.5cm}[1]$$
with $$$S_0$$$ the non-diffusion weighted signal, $$$b_i$$$ and $$$g_i$$$ the diffusion weighting strength and direction respectively, $$$f$$$ the free water signal fraction, $$$D$$$ the diffusion tensor and $$$d=3\hspace{2mm}\mu m^2/ms$$$ the diffusivity of free water at body temperature. To solve this ill-conditioned problem, a relationship between the signal fraction $$$f$$$ and the actual volume fraction $$$F$$$ is introduced by accounting for the T2-values of the tissue $$$T2_{tissue}$$$ and free water $$$T2_{fw}$$$:
$$f=\frac{F\cdot e^{-TE/T2_{fw}}}{\left(1-F\right)\cdot e^{-TE/T2_{tissue}}+F\cdot e^{-TE/T2_{fw}}}.\hspace{1.5cm}[2]$$
$$$T2_{tissue}$$$ and $$$T2_{fw}$$$ can either be estimated or assumed to be known a priori. Estimation of the T2 relaxation times can be achieved from pure voxels from complementary data7 or even from the non-diffusion weighted images. For equation [2] to be useful, diffusion measurements with at least two different TEs need to be acquired.
In this work, first the Cramér-Rao lower bound (CRLB) was used to evaluate the FWE-T2 model with and without a priori known T2 values. The CRLB, which was derived assuming Gaussian distributed data, gives insight in the maximal attainable precision of the parameter estimates and their correlations. Note that for Gaussian distributed data the NLS estimator equals the maximum likelihood estimator, which is known to attain the CRLB asymptotically9. Next, the optimization space of the NLS estimator of the FWE-T2 model was studied together with corresponding Monte Carlo simulations. For both the FWE-T2 model and the standard FWE model, 1000 data sets contaminated with zero mean Gaussian distributed noise were generated. Finally, the effect of errors in the T2 assumptions on the resulting model parameter estimates was studied. Simulation settings include: SNR=20 on the non-diffusion weighted signal and $$$b=0,0.5,1\hspace{2mm}\mathrm{ms/\mu m^2}$$$ with 30 measurements per shell and 6 non-diffusion weighted images. For the FWE-T2 model, 2 TE values were used: TEmin = 65ms and a variable TEmax.
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