Elisabeth Jonckers1, Michael Belloy1, Dany Dsouza2, Marleen Verhoye1, Thomas Mueggler2, and Annemie Van der Linden1
1Bio-Imaging Lab, University of Antwerp, Wilrijk, Belgium, 2Roche Pharma Research and Early Development, Roche Innovation Center Basel, Basel, Switzerland
Synopsis
Our pre-clinical rodent study showed an increased
Functional Connectivity at the level of the striatum and the cingulate cortex induced
by cocaine injection. The effect was detectable 4 hours after administering
cocaine and sustained for 24h.
Introduction
In
this experiment we targeted the dopaminergic system using cocaine hydrochloride
in a “binge” application paradigm that has revealed pronounced increases in
Delta-, Theta-, and Alpha-Band EEG Power Spectra (1). Since Rs-fMRI networks are demonstrated to
correlate with the EEG outcome, we expect to observe modulated rs-fMRI networks
with this paradigm.Methods
A resting
state fMRI experiments was conducted in 8 control and 7 cocaine-treated Wistar
rats anesthetized with medetomidine (Domitor, Pfizer, Germany; bolus 0.05 mg/kg
+ continuous infusion 0.1 mg/kg/h, SC). The data were acquired on a 9.4T
Biospec scanner (Bruker, Ettlingen, Germany) using a GE-EPI sequence with a
repetition time (TR) of 2000ms, and echo time (TE) of 16 ms. Twelve axial
slices with a slice thickness of 1mm and an in plane resolution of (0.23x0.23)
mm2 were scanned 150 times, resulting in
a measuring time of 5 min.
At
Day 1 a baseline scan (BL) and a post (3) injections scan were acquired. Three
consecutive injections of Cocaine hydrochloride (Sigma-Aldrich) i.p. with a dose
of 15 mg/kg or 3 saline injections were given 1 hour apart. Four hours after
the last injection the first post treatment scan was acquired (D1) and at day 2
(24 hours after the last injection) the second (D2). Standard pre-processing
was carried out using the Statistical Parametric Mapping 8 program (SPM 8). Independent
Component Analysis was done using the GIFT toolbox (Group ICA of fMRI toolbox:
http://icatb.sourceforge.net/), working in Matlab2008 (www.mathworks.com) with a
preset of 15 components. Additionally REST time courses were extracted for seed
regions based on the ICA outcome. Functional connectivity (FC) maps were
generated in SPM8. Both motion parameters, as well as global signal time courses
were regressed out during this analysis to improve the specificity of the FC (2). Statistics were performed using repeated
measures ANOVA. The statistical outcome was FWE corrected and only clusters of
more than 8 voxels were taken into account.
Results
A
distinct number of components could be defined co-inciding with anatomical
relevant regions in convergence with our previous reports (3) (fig 2). Statistical significant effects were only
found in the cocaine treated animals in the cingulate cortex and striatum. Both
post treatment time-points showed a significant difference compared to the
baseline scan (p=0.009 and p=0.027 resp; FWE corrected) (shown for the
cingulate cortex in figure 3). Comparing cluster sizes shows an increase after
cocaine injection for both components (fig 4). Additionally, our ICA results
were confirmed with seed based analysis for the right cingulate cortex (P=0.001;
FWE corrected).Discussion and Conclusion
The outcome showed a change in
local FC at the level of the cingulate cortex and striatum (shown in figure 2)
after acute cocaine hydrochloride injection. This effect on FC was still present
24h after the last injection indicating long-lasting neuro-circuitry changes independent of acute
drug exposure. In fact, even at 4h we expect cocaine brain exposure is close to
0, in line with its pharmacokinetic properties (T1/2brain +/-15min) (4). Hence, both the acute and long lasting change in FC
is reflecting plasticity in striatal and cortical networks in response to a cocaine
binge paradigm.
Our results clearly indicate that:
(A) the FC effects of cocaine, detectable 4 and 24h after drug dosing are
localized since only two components show a significant difference; (B) the
differences in FC are persistent beyond the expected brain exposure of cocaine and
normalization of the brain circuits may take longer than the wash-out period
chosen in our study; (C) A follow-up study is required to test whether the
effect is transient and at what time point normalization in FC in the two brain
regions occurs.Acknowledgements
No acknowledgement found.References
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