Printed magnetic ink creates predictable B0 field perturbations based on printed shape and magnetic susceptibility. This can be exploited for contrast in MR imaging techniques that are sensitized to off-resonance, such as fat-suppressed imaging with spectral presaturation. Magnetic ink therefore has the potential to be used in temporary tattoos for creating MR-visible skin markings of arbitrary shape and size, with applications in surgical planning, radiation therapy, tracking of joint movement, or other image registration scenarios. Here we characterize the susceptibility variations of magnetic ink and demonstrate application for MR-visible skin markings.
A 1”-diameter circle was printed with MICR toner on standard printer paper, which was trimmed, laminated, and submerged in water (Figure 1). Coronal scans of the phantom were acquired on a GE Discovery MR750 3T scanner with an 8-channel cardiac coil. Two 3D gradient-echo scans (ΔTE = 0.5 ms) with 1 mm isotropic resolution were acquired for B0 field mapping4,5. Gradient-echo scans with a spectral presaturation pulse (590 Hz bandwidth) were also acquired, with the saturation frequency adjusted over the range -500 to 500 Hz.
Simulated B0 field maps (1 mm isotropic resolution) were generated by convolving the volume-average magnetic susceptibility distribution (χ) of the ink with a dipole kernel2,6, where χ was chosen to minimize the mean squared error between the acquired and simulated field maps in high-signal areas near the center of the phantom.
Coronal 3D breath-held gradient-echo scans with saturation pulses tuned to suppress fat, both fat and water, or neither were acquired in a healthy male volunteer with a magnetic ink pattern printed on paper pressed against the skin of his chest. Higher resolution, sagittal 3D gradient-echo scans with saturation pulses tuned to suppress fat, water, or both were acquired in a healthy volunteer with temporary tattoos printed in magnetic ink applied to the skin of the calf (Figure 5).
The saturation frequency can be tuned to highlight desired regions of the geometry-dependent B0 distortions due to the ink pattern by specifying the resonance frequencies that are suppressed, producing images with different contrasts (Figure 1).
In this phantom, a volume-average estimate of χ=130 ppm minimized the mean squared error. Assuming a 10 μm thickness of ink, this implies χ=13,000 ppm in the ink. Signal line plots comparing the sagittal acquired vs. simulated field maps corresponded well (Figure 2). The signal line plots in the coronal plane corresponded well at the circle’s center, but not at its edges (Figure 3). This discrepancy may be due to the resolution of the simulation. The correspondence also worsened towards the edges of the phantom due to the interface with air.
The field distortions of the magnetic ink pattern pressed to the skin of a human subject were visible with different contrasts as the saturation frequency was adjusted, similar to phantom results (Figure 4). Due to the off-resonance, the pattern was visible in the images where both fat and water were suppressed, and produced signal voids in images where neither was suppressed. The vertical lines in the pattern were not visible because they were parallel to the B0 field. The signal in the patterns was brighter than the other tissues because it came from subcutaneous fat.
The temporary tattoos applied to the calf were visualized with a 3D maximum intensity projection (MIP) of the images acquired with fat suppression (Figure 5). Colored arrows indicate which patterns in the photos correspond to which patterns in the MIP. The patterns appeared brighter than the other tissues because the signal came from subcutaneous fat. However, the visibility of the tattoos varied from one side of the leg to the other, perhaps due to B0 variations across the leg that could be improved with shimming.
Although there have been several reports of tattoos causing burning or heating in MRI7, neither of the scanned subjects reported any heating or burning sensation due to the ink during scanning. Loop-shaped patterns were avoided until a more careful characterization of heating can be performed.
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