Diffusion-weighted dual-echo steady-state (DW-DESS) imaging allows multiple MR parameters to be quantified without image distortion. In this work, we investigate the effects of B1 correction on the accuracy of T1, T2 and ADC parameters estimated from DW-DESS. We extend the quantification to species with ADC and T2 values that are similar human cancers by using a novel phantom mixture. The accuracy of parameter estimates measured with DW-DESS is improved after B1 correction, with correlation coefficients of 0.912, 0.997 and 0.778 without B1 correction to 0.993, 0.998 and 0.947 after B1 correction (for T1, T2 and ADC, respectively).
Diffusion-weighted echo-planar imaging (DW-EPI) is prone to image distortion caused by field inhomogeneity and local susceptibility variations. An alternative approach is to use diffusion-weighted steady-state free precession (DW-SSFP) sequences to obtain high-resolution, distortion-free DW images1-3. With an additional echo, the dual-echo steady state (DW-DESS)4-7 sequence can allow T1, T2 and ADC maps to be obtained without distortion.
Variation in the transmitted radiofrequency field, B1, was noted in previous studies as a possible source of error in the quantification of parameters in DW-DESS. In recently published work by Gras et al.7, DW-DESS parameter estimates included B1 correction with an actual flip-angle imaging (AFI) sequence8.
Purpose: We further quantify the incremental effects of B1 correction on the accuracy of T1, T2 and ADC values estimated with DW-DESS. In the study by Gras et al, relatively high ADC values (>2000 um2/s) were investigated. In this work, we extend the quantification to lower ADC ranges that are better matched to those measured in human cancers (≈500 to 1200 um2/s) using a novel phantom mixture that allows both the ADC and T2 to be within the range of human lesions.
Phantom mixtures:
Mixtures of manganese chloride (MnCl2), agar and polyvinylpyrrolidone (PVP, Sigma-Aldrich) were used. PVP alone9,10 leads to ADC values in the range of human lesions but with T2 values that are too high. Here, MnCl2 was added to PVP to reduce the T2 relaxation times.
MR Imaging:
Experiments were performed on a 3T scanner (MAGNETOM Prisma, Siemens Healthcare, Erlangen, Germany) at 0°C using an ice-water phantom bath.
DW-DESS: A 3D sequence was implemented and acquired with TR=23.5ms, TE1,2=5,16.3ms, FOV=294×294mm2, matrix=256×256, 16 slices, 3mm thickness, diffusion duration=5ms. Two sets of data were acquired: a) flip angle=15° with Gdiffusion=30mT/m, and b) flip angle=35° with Gdiffusion=5mT/m. The scan duration was 3min for both sets.
B1 map: A 3D AFI sequence was implemented and scanned with nominal flip=60°, TR1,2=30,150ms, spoiling areas A1,2 = 450,2250 mT∙ms/m, phase increment=34°, scan duration 12min.
Comparison scans:
DWI: ADC measurements were performed with a readout-segmented EPI (Siemens, RESOLVE) sequence with parameters: TR/TE = 3930/52ms, FOV=225×225mm2, 110x110 matrix, 3mm thickness, 16 slices, 3 diffusion directions, b-values= {0,100,300,600,1000}, scan duration 4min.
T2 mapping: T2 relaxation times were estimated with the Siemens T2 MapIt turbo spin-echo sequence with TR=3000ms and TE={11, 23, 34, 45, 57, 68, 79, 90, 102, 113, 124, 136} ms.
T1 mapping: T1 relaxation times were measured using the Siemens T1 MapIt sequence with parameters TR/TE=5.4/2.5 ms, flip angle=3° and 15°.
Image Analysis:
T1, T2 and ADC values were simultaneously estimated by fitting the DW-DESS echoes to a signal model based on extended phase graph (EPG) modelling11,12. All simulations and analysis were performed in MATLAB. DW-DESS-parameter estimates for each ROI were estimated with and without B1 correction. For B1 correction, the measured flip angles from the AFI scan was incorporated into the signal model for fitting.
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