Introduction to Resting-State fMRI & Functional Connectivity
Thomas Yeo1

1National University of SIngapore

Synopsis

In this education workshop, I will motivate the use of resting-state fMRI (rs-fMRI) and functional connectivity to study the human brain. I will also present example studies that use rs-fMRI as a tool to investigate brain organization, disorder and behavior. I will conclude with some existing challenges about rs-fMRI.

Highlights

1. We can understand the brain in terms of specialization and integration

2. Functional integration can be studied with resting-state fMRI (rs-fMRI), which allows us to answer questions about brain organization, disorder and behavior

3. While there are unresolved caveats, rs-fMRI promises to be important for data-driven discovery science

Target Audience

Faculty and trainees who are interested in using resting-state fMRI for their studies.

Objectives

To understand how resting-state fMRI can be utilized to study healthy brain organization, diseases and behavior notwithstanding certain caveats that have arisen recently.

Synopsis

We can try to understand the brain in terms of functional specialization and integration [1]. While task-based fMRI allows us to study functional specialization, connectivity allows us to study integration [1]. The “resting” brain dynamically fluctuates during rest. Bharat Biswal was the first to demonstrate that resting-state fMRI (rs-fMRI) fluctuations within the motor system are correlated [2]. The correlations between fMRI time courses are called functional connectivity and are thought to reflect multisynaptic connectivity [3, 4]. Intriguing, there are strong correspondences between networks recruited during tasks and networks of regions functionally connected during rest [5, 6, 7]. The correspondences suggest that we can use rs-fMRI to extract functional brain networks [5, 8, 9] instead of having to run multiple task-based fMRI sessions with different tasks. These networks can be used to study healthy brain organization, diseases and behavior [10, 11, 12]. However, it is worth noting that rs-fMRI is not the panacea that people originally thought. Even though rs-fMRI is “task-free”, it is affected by motion [14], fatigue [15] and internal thought processes during rest [16]. This can introduce biases for example when studying aging because older people move more, are more easily fatigued and have different kinds of thoughts when compared with young people. Nevertheless, the enthusiasm over rs-fMRI has resulted in large-scale rs-fMRI datasets that are collected in roughly the same way (e.g., [16, 17, 18]), providing great opportunity for data-driven discovery science [19, 20].

Acknowledgements

No acknowledgement found.

References

1. Friston KJ (1994) Functional and effective connectivity in neuroimaging: a synthesis. Human brain mapping 2: 56-78

2. Biswal B et al. (1995) Functional connectivity in the motor cortex of resting human brain using echo-planar mri. Magnetic resonance in medicine 34: 537-541

3. Vincent JL et al. (2007) Intrinsic functional architecture in the anaesthetized monkey brain. Nature 447: 83-86

4. Lu J et al. (2011) Focal pontine lesions provide evidence that intrinsic functional connectivity reflects polysynaptic anatomical pathways. The Journal of Neuroscience 31: 15065-15071

5. Smith SM et al. (2009) Correspondence of the brain's functional architecture during activation and rest. Proceedings of the National Academy of Sciences 106: 13040-13045

6. Krienen FM et al. (2014) Reconfigurable task-dependent functional coupling modes cluster around a core functional architecture. Phil. Trans. R. Soc. B 369: 20130526

7. Bertolero MA et al. (2015) The modular and integrative functional architecture of the human brain. Proceedings of the National Academy of Sciences 112: E6798-E6807

8. Yeo et al. (2011) The organization of the human cerebral cortex estimated by intrinsic functional connectivity. Journal of neurophysiology 106: 1125-1165

9. Power et al. (2011) Functional network organization of the human brain. Neuron, 72: 665-678

10. Buckner RL et al. (2011) The organization of the human cerebellum revealed by intrinsic functional connectivity. J Neurophysiology 106:2322-2345

11. Zhang D & Raichle ME (2010) Disease and the brain's dark energy.Nature Reviews Neurology 6:15-28

12. Fox MD & Greicius M (2010) Clinical applications of resting state functional connectivity. Frontiers in systems neuroscience 4:19

13. Smith et al. (2015) A positive-negative mode of population covariation links brain connectivity, demographics and behavior. Nature neuroscience 18:1565-1567

14. Power JD et al. (2012) Spurious but systematic correlations in functional connectivity MRI networks arise from subject motion. Neuroimage 59:2142-2154.

15. Yeo BTT et al. (2015) Functional connectivity during rested wakefulness predicts vulnerability to sleep deprivation. NeuroImage 111:147-158

16. https://en.wikipedia.org/wiki/Human_Connectome_Project

17. http://neuroinformatics.harvard.edu/gsp/

18. http://fcon_1000.projects.nitrc.org/

19. Biswal et al. (2010). Toward discovery science of human brain function. Proceedings of the National Academy of Sciences 107: 4734-4739.

20. Gray J (2009) The fourth paradigm: data-intensive scientific discovery



Proc. Intl. Soc. Mag. Reson. Med. 24 (2016)