Physicists and clinicians involved in the field of neuromuscular disordersThe pathological process in neuromuscular disorders leads to a variety of alterations within muscle fibers including atrophy, inflammation, fatty infiltration … and nerves. Over the last decade, dedicated MRI tools have been designed in order to quantify more or less specifically those abnormalities with the aim of providing sensitive biomarkers which could be used for diagnostic and therapeutic follow-up purposes. The corresponding tools and their corresponding limitations will be presented in the first part of the talk. Once the MR images are acquired a large amount of work is needed if one aims at tracking these abnormalities at the level of individual muscles or muscle groups. While segmentation tools have been largely developed for central nervous system, the applicability of these tools for skeletal muscle has been poorly investigated. The second part of the talk will be dedicated to the presentation of the multiple segmentation approaches, including those related to atlas-based segmentation pipelines, which have been developed for segmentation of muscle MR images. The approaches will be illustrated regarding their potential for longitudinal follow-up in healthy volunteers and patients with neuromuscular disorders.