High angular-resolution diffusion imaging (HARDI) has advantages over Diffusion Tensor Imaging in its ability to resolve crossing fibers and distinguish neuronal tracks with higher turning angles [1, 2]. These features of HARDI are crucial for identifying and characterizing the microstructural changes in neuro pathology and traumatic brain injury [3]. HARDI has already been shown to improve fiber resolution in adults [4]. However, the main short-coming of HARDI is the extended scan time, which can be more than four times of standard DTI of similar spatial resolution. The longer scan time is already problematic in adults leading to higher rates of acquisition failure – such as increased movement and patient fatigue – and made more challenging in children. Multiband MRI techniques have been used to reduce scan time. In this study we present the preliminary fiber tractography of healthy neonatal and pediatric subjects acquired using multiband multi-shell HARDI sequence.A total of 10 heathy pediatric subjects (6-17 years 3 females, 7 males) and 12 healthy neonates (1-6 weeks, 4 males, 8 females) were scanned on a 3T Skyra (Siemens AG, Erlangen, Germany) using 32-channel head coil. The multiband multi-shell HARDI acquisition consisted of four modular complementary sequences, each acquiring the volumes as three simultaneous bands in tandem. The sequences are divided into two B-value (B=5000 and B=3000), and two sets of 128-unique directions (total of 256 unique directions at each B value). The sequence is EPI with following parameters: FOV=240mm, voxel size=2.4mm (isotropic), matrix size=100x100x60, TE/TR=130ms/3600ms. Each sequence is around 240 ± 15 seconds, and the entire acquisition can be accomplished in under 16 minutes. Image reconstruction, motion correction, and pre-processing were conducted using a custom image processing pipeline internally developed (Scheider Laboratory, Pittsburgh PA, U.S.A) [5]. Fiber tracking and preliminary comparison analysis in post-processed images were done on DSI studio [6, 7]. All pediatric subjects and 75% of neonates tolerated and completed the scan. For each subject, the following 16 tracts were analyzed: genu, body, and splenium of corpus callosum (CC); fornix; corona radiata; thalamic radiations; corticospinal tract; external capsule; frontal aslant; arcuate; superior and inferior longitudinal fasciculus; optic radiations; inferior fronto-occipital fasciculus; cingulum; and uncinated. Most tracts showed no observable differences between youngest and oldest subjects. Compared to Healthy Adults, sparse frontal connectivity across all pediatric scans was observed (Figure1). Increased visualization of fornix in younger age group (Figure2A), and increased lateralization of all segments of CC in older age group (Figure2B). With a few exception, no graph abnormalities were observed compared to healthy adult controls.This study showed that a multiband multi-shell sequence could acquire HARDI images at a scan time practicable for pediatric patients. The visualization of tracts in younger subjects were comparable to the adults. The quality of fiber tracking is comparable to healthy adult controls, and superior in some cases such as the fornix. An in depth study and quantitative analysis with more healthy subjects are required to characterize and standardize HARDI based fiber tracking in younger subjects.