Whole-brain volume alteration and its correlation with anxiety severity in patients with obsessive compulsive disorder and generalized anxiety disorder
Gwang-Won Kim1, Chung-Man Moon1, Tae-Hoon Kim1, and Gwang-Woo Jeong1,2

1Research Institute of Medical Imaging, Chonnam National University Medical School, Gwang-ju, Korea, Republic of, 2Department of Radiology, Chonnam National University Medical School, Gwang-ju, Korea, Republic of

Synopsis

Obsessive compulsive disorder (OCD) and generalized anxiety disorder (GAD) are associated with abnormalities in the processing and regulation of anxiety. The purpose of this study was to evaluate gray matter (GM) and white matter (WM) volume alterations over whole-brain structures in healthy controls vs. patients with OCD vs. GAD using voxel-based morphometry (VBM), and further to assess the correlations of the GM and WM volume variations with the scores for anxiety severity in OCD and GAD.

Subjects and methods

Sixteen patients with OCD (mean age = 31.3±10.2 years), 16 patients with GAD (33.0±8.6 years), and 16 healthy controls (32.5±7.3) participated in this study (Table 1). The patients with OCD were assessed using the Clinical Global Impression-Severity Scale (CGI-S), Hamilton Rating Scale for Anxiety (HAM-A), Hamilton Rating Scale for Depression (HAM-D), and Yale-Brown Obsessive Compulsive Scale (Y-BOCS) (Table 1). The patients with GAD were assessed using the CGI-S, HAM-A, HAM-D, Anxiety Sensitivity Index-Revised (ASI-R), and Generalized Anxiety Disorder Scale 7 (GAD-7) (Table 1). Magnetic resonance examination was performed on a 3.0-T Magnetom Verio MR Scanner (Siemens Medical Solutions, Erlangen, Germany) with a 12-channel birdcage head coil. The T1-weighted sagittal images were acquired using a three-dimensional magnetization prepared rapid acquisition gradient echo (3D-MPRAGE) pulse sequence with the following parameters: repetition time/echo time = 1900 ms/2.35 ms, field of view = 220×220 mm2, matrix = 256×256, slice thickness = 1 mm, and slices = 176. MRI data were post-processed using Statistical Parametric Mapping software (SPM8) with the diffeomorphic anatomical registration through an exponentiated Lie algebra (DARTEL) algorithm.

Results and discussion

To our knowledge, this is the first study to evaluate the GM and WM volume abnormalities between patients with OCD and GAD using DARTEL-based VBM. The main findings were as follows: (1) patients with OCD showed GM atrophy in the anterior cingulate cortex (ACC), orbitofrontal gyrus (OFG), superior temporral gyrus (STG), and angular gyrus (AG) compared with healthy controls (Table 2, Fig. 1); (2) patients with GAD showed GM atrophy in the ACC, superior occipital gyrus (SOG), precuneus, OFG, dorsolateral prefrontal cortex (DLPFC), medial prefrontal cortex (mPFC), superior temporal gyrus (STG), insula, and AG, and WM atrophy in the DLPFC, mPFC, and OFG compared with healthy controls (Table 2, Fig. 1); (3) compared with patients with OCD, patients with GAD showed GM atrophy in the SOG and mPFC, and showed WM atrophy in the mPFC and AG (Table 2, Fig. 2); and (4) GM and WM volumes of the mPFC and DLPFC in patients with GAD were negatively correlated with HAM-A scores (Fig. 3).

Conclusion

Our findings indicate that morphometric deviation of the mPFC in patients with GAD may be associated with anxiety disorder. This study will be useful to understand the neuropathology of OCD and GAD in terms of GM and WM abnormalities.

Acknowledgements

This research was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIP) (2015R1A2A2A01007827) and the Ministry of Education (2014R1A1A2006730).

References

1. Moon et al, Neuroreport. 2014;25:184-189

2. Park and Jeong, Psychiatry Clin Neurosci 2015;69:717-723

Figures

Table 1.

Demographic and clinical characteristics of patients with OCD and GAD, and healthy controls


Table. 2.

Brain areas with significant alterations in GM volume between 3 groups using ANOVA (p<0.001) and post-hoc t-test (p<0.001)


Fig. 1.

Differential GM (a) and WM (b) volume alterations in patients with OCD and GAD: post–hoc t-test (p<0.001). Green color, controls > OCD; red, controls > GAD; yellow, the intersection set of {controls > OCD} and {controls > GAD}.


Fig. 2.

Decreased GM (a) and WM (b) volumes in patients with GAD compared to patients with OCD: post–hoc t-test (p<0.001). The color-coded pixels were scaled to the range more than cut-off threshold (p<0.001).


Fig. 3.

The GM volumes (a) of the mPFC (Spearman’s rho= -0.69, p= 0.003) and DLPFC (rho= -0.64, p= 0.007) and the WM volumes (b) of the mPFC (rho= -0.61, p= 0.013) and DLPFC (rho= -0.66, p= 0.005) in patients with GAD were negatively correlated with HAM-A scores.




Proc. Intl. Soc. Mag. Reson. Med. 24 (2016)
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