Synopsis
We describe the evolution of chronic multiple sclerosis
lesions from a quantitative MR imaging and spectroscopy perspective. Metabolite
concentrations were obtained along with measures of lesion T1-hypointensity and
size. Moderately hypointense lesions were more metabolically active than
severely hypointense lesions, driving an increase in the glial marker
myo-inositol. Correlational analyses revealed that lesion size is a better
predictor of axonal health than T1-hypointensity, with lesions larger than 1.5
cm3 exhibiting terminal axonal injury. A positive correlation between changes
in choline and in lesion size in moderately hypointense lesions implied that changes
in lesion size are mediated by chronic inflammation.Introduction
Lesions
are the radiological hallmark of multiple sclerosis (MS), but T1- and
T2-weighted imaging cannot define their pathophysiology. Proton
MR spectroscopy (
1H-MRS) can assess tissue metabolism, but it suffers from limited spatial resolution. Large voxel size introduces uncertainty in studying all but the largest
lesions, because of contamination by cerebro-spinal-fluid (CSF) and
normal-appearing tissue. Another limitation of conventional
1H-MRS is the use of metabolic ratios that mask changes occurring in both the numerator and denominator. Here we use
absolute quantification with stringent partial volume correction, combined with
0.75 cm
3 spatial resolution
1H-MRS imaging (
1H-MRSI), to study MS lesions over a 3-year, semi-annual follow-up. Concentrations of
N-acetyl-aspartate
(NAA), creatine (Cr), choline (Cho) and
myo-inositol (mI) are obtained along
with quantitative measures of lesion T1-hypointensity and size. We studied the changes of these metrics
over time, as well as their cross-sectional and longitudinal dependence.
The goal was to fully characterize the metabolism associated with chronic lesions' evolution and to define the metabolic correlates of the conventional
measures of lesion status.
Methods
Subjects: 10
relapsing-remitting MS patients within 6 years from diagnosis, scanned semi-annually for 3 years.
Data acquisition: pre- and post-contrast T1-weighted
MRI (MP-RAGE), T2-weighted MRI (FLAIR), B
0 shimming, 10×8×4.5 cm
(AP×LR×IS)=360 cm
3 1H-MRS VOI (PRESS
TE/TR=35/1800 ms), encoded to 480
voxels, each 1.0×1.0×0.75 cm
3 (
Fig. 1A-C).
Segmentation:
Lesion masks were obtained from FLAIR
1 and gray, white matter (GM, WM) and CSF masks
were segmented from MP-RAGE
2.
Co-registration: FLAIR was co-registered to
the MP-RAGE and the transformation matrix was applied to the lesion mask co-registering
it to the MP-RAGE space (
Fig. 2B). Mis-registered pixels were deleted (
Fig.
2C). All masks, now in MP-RAGE space, were co-registered with the
1H-MRSI,
yielding their volume in every
1H-MRSI voxel.
Lesion
characterization: Based on their MP-RAGE contrast, lesions were quantitatively
defined as isointense or hypointense, and within the latter group: as
severely
hypointense (SH) and
moderately hypointense (MH). A T1-contrast
ratio was calculated for each lesion as a continuous measure of T1-hypointensity
3.
Metabolic quantification: Phantom replacement with lesion-specific T
1
and T
2 relaxation times
4,5.
1H-MRSI quality control and partial volume
considerations: To maximize lesion inclusion and control partial volume “voxel
shifting
6” was performed for each lesion (
Fig 1D-E). Only voxels with >40%
lesion, <30% CSF, <30% GM; metabolite Cramer-Rao lower bounds<20% and 4<linewidths<13
Hz were retained. Metabolite concentrations were corrected for partial CSF volume.
Statistics:
ANCOVA, random coefficients regression and Pearson correlations with 2-sided p values. Analyses controlled for variable lesion volume in the
1H-MRS voxels.
Results
Eighteen unique lesions providing 89
measurements were studied. All were T1-hypointense (6 SH, 12 MH) (Fig. 3A-B).
Cross-sectional
findings:
Fig.
3C: Cr was higher in MH than in SH
lesions (6.0 millimolar±1.3 vs. 5.0±1.4, p=0.05). There was a statistical trend of lower NAA in SH
versus MH lesions (p=0.06).
Fig.
4: NAA concentration was inversely related to lesion size (r=-0.3,
p=0.04). A trend for such relationship was also found for Cr (r=-0.3, p=0.06).
Longitudinal findings:
Fig.
5A: An increase in mI (0.03 mM/month, p=0.02), driven by its change in MH
lesions (statistical trend: 0.03 mM/month, p=0.06). 5B: A decrease in T1-contrast ratio (-0.001/month, p=0.03), also driven by its change in MH lesions (-0.001/month, p=0.04).There were
trends for increasing NAA in MH lesions (0.03
mM/month, p=0.07) and for size increase in the SH lesions (0.01
cm3/month, p=0.06). Rates of change in lesion size were strongly correlated with changes in Cho, for MH lesions (r=0.8, p=0.01).
Discussion
Cross-sectional findings:
The findings among SH and MH lesions (Fig. 3) are in line with previous 1H-MRS3,7,8
and histopathology9,10, indicating that very hypointense lesions suffer more
neuronal damage than less hypointense ones. Here we show, however, that NAA and Cr decrease as a function
of T1-hypointensity only within SH lesions (Fig. 4), and that this relationship is mediated by lesion size,
which is a better predictor of low NAA than T1-hypointensity. Additionally, lesions
larger than 1.5 cm3 exhibited very narrow (low) NAA concentration
range, indicating terminal axonal injury.
Longitudinal findings:
The increase in mI (Fig. 5A) indicates astrogliosis, a histopathological
hallmark of the MS lesion11. The correlation between changes in Cho and in
lesion size implies lesion size is mediated by chronic inflammation. The trend
for NAA increase may indicate medication effects, as hypothesized previously12-14.
The observed changes in T1-hypointensity (Fig. 5B) and size indicate that decrease in
lesion T1-hypointensity precedes enlargement.
Conclusion
The
results shed light on the metabolism of lesion evolution, and
inform about the metabolic correlates of lesion appearance on conventional MRI,
as encountered in clinical practice.
Acknowledgements
This work was supported by NIH grants NS050520,
NS29029, EB01015 and the Center for Advanced Imaging Innovation and Research
(CAI2R, www.cai2r.net), a NIBIB Biomedical Technology Resource Center (NIH P41
EB017183).
Assaf Tal acknowledges the support of the Monroy-Marks
Career Development Fund, the Carolito Stiftung Fund, the Leona M. and Harry B.
Helmsley Charitable Trust and the historic generosity of the Harold Perlman
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