On the arterial spin label (ASL) imaging, the bright intravascular signal contributes to the overestimation of perfusion signal. ASL with Motion-sensitized Driven-equilibrium (MSDE) (1) has been used to suppress the vascular signal, however, it is sensitive to B1 and B0 field uniformity as well as eddy current. DANTE (Delays Alternating with Nutation for Tailored Excitation) preparation pulse (2) is an effective flow signal suppression method that does not affect static tissue signal. In this study, DANTE-enabled ASL sequence was prepared and evaluated using phantom and healthy human subjects to see whether DANTE preparation would bring a sufficient vascular suppression into effect comparing to MSDE method.DANTE pulse train was inserted between background suppression pulses as shown in Figure1. The parameters for DANTE module were as follows: RF pulse duration=100us, Tp: 1ms, Number of DANTE pulses = 200. For ASL, the following parameters were used: FOV = 240 x 240 mm, slice thickness = 4mm, # of slices = 32, TE= 10msec, Post labeling time Delay= 700msec, labeling durations= 3sec, acquisition = 7 arms x 512 sampling points. After the ASL sequence, a reference image without ASL module was acquired to provide M0. All scans were performed on a 3.0 Tesla Discovery750 (GE Healthcare) with 32-channel Receive only head coil. Phantom experiment: A flow phantom consisting of static water chamber and flow tubes which run through the chamber was used. 2.5 cm/s water flow was generated in the tubes. T1 value measured inside the static chamber was 2442msec, and the flowing part was 2054msec. The flip angle (FA) of DANTE RF pulse was changed to 0, 2, 4, 6, 8, 10, 12.5, and 15 [degree], and the gradient area of 28.6 [msec·mT/m] were used. To compare DANTE against MSDE, ASL scan using MSDE (1) was performed with the b value of MSDE varying from 0.005, 2.5, 5.0, 7.5, up to 10.0 [sec/mm$$$^2$$$]. Static water signal was measured inside the static chamber area on the reference image. Flow signal was measured inside the flow tubes on the ASL image. Volunteer study: A healthy volunteer was scanned with DANTE-ASL and MSDE-ASL. The study was approved by the local IRB and written informed consent was obtained from all subjects. NEX of two was used for human scan and the rest of ASL parameters were kept the same as phantom scan. The b value of 10 [sec/mm$$$^2$$$] was used for MSDE, and FA of 10 [degree] and the gradient area of 28.6 [msec·mT/m] were used for DANTE. The signal uniformity and intensity of DANTE-ASL and MSDE-ASL images were compared and evaluated against each other.The changes in signal intensity measured in the phantom images with MSDE or DANTE are shown in Figure 2. The signal decay patterns of flow component were similar in both DANTE and MSDE. As for the signal decay in the static area, rapid signal reduction was observed around b=7.5 [sec/mm$$$^2$$$] with MSDE preparation while gradual signal decay along FA change was seen with DANTE. The signal intensity ratio of DANTE with FA of 5 [degree] was similar to MSDE with b value of 5 [sec/mm$$$^2$$$] in the case of 2.5 cm/s flow. Similarly, DANTE with FA7.5 [degree] was close to MSDE with b value 10 [sec/mm$$$^2$$$]. Figure.3 shows the comparison of human ASL images without vascular suppression preparation, with MSDE, and with DANTE preparation pulse. The signal intensity ratio of the bilateral gray matter was 1.23 with MSDE, and 1.07 with DANTE. The signal intensity ratio of the bilateral gray matter was not as uniform in MSDE as DANTE. It was also observed that vascular signal was decreased with both methods.It was demonstrated that the proposed DANTE- ASL imaging worked for both phantom and human imaging. DANTE-ASL reduced the intravascular signal while keeping the signal uniformity whereas conventional MSDE preparation generated less uniform ASL images. Local B1+ inhomogeneity caused signal loss (3) and nonuniformity with MSDE sequence. DANTE uses a small FA pulses resulting in smaller B1+ inhomogeneity. The results suggest that DANTE preparation works for reducing the intravascular signal for ASL. In conclusion, we have demonstrated that the DANTE preparation can be used as a vessel signal suppression method for ASL.